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The map label for this gene is tig [H]

Identifier: 13508070

GI number: 13508070

Start: 388923

End: 390257

Strand: Direct

Name: tig [H]

Synonym: MPN331

Alternate gene names: 13508070

Gene position: 388923-390257 (Clockwise)

Preceding gene: 13508069

Following gene: 13508071

Centisome position: 47.64

GC content: 39.25

Gene sequence:

>1335_bases
ATGAAACAATACAAGCTTGTCAATACAACCCAAAAAGAAAAGACGCTTTGTCTTGAAATAGCAATTGACACGAAGCTGTG
AAAGGAGACACAGCAAAAGCAAACCCAAGATTTAACCAAAAACATGAAAATCAAAGGTTTTCGTAAAGGTAAAGTGCCTC
CAACTTTAGCTAAGGATTACTTAGACCGTGCCGAGTTGTTGCAACGTAGCGCCCAAGCGGTAATTGATGCCATCTTCCAG
CCGTTACAACAAGAAGCAGTAATTGCTGACAACGAGAATGTAATTGAGGACTTTCCTACCATTGACTTTAAAACAATCAA
TGAGAACGATTGTGTTATTCTGTTTGACTTTGATCTCGTACCCCAGTTTGAACCACCTGATTACAAGCACATCAAGGATT
TAAGCCCGATTGTACCCCTAAAGGATGAGGAGTTCAACAAGGAGTTGCACAACATCGAAAAGAACAAAGGGAAACTAGTT
GATGTTAGTGACAAAGCATTGGCCAACAATGATATTGCCGTCATTGACTTTGTTGGCAAGGTAGATGGTAAGGTGCTTGA
AAGCGCTACTGCCAAACAATACGAATTAACGATTGGCTCCAACAGCTTTATTGATGGCTTTGAAAGTGGTTTAATTGGCA
TGAAAGTAGGGGACAAGCGTCAGTTAAAGTTAAAGTTCCCCAAGGACTACCATGCTGAAGAATTGAAGGGTAAACCAGTA
GAGTTTGACATAGAGCTCAAAGCAATTAAGCAGTTAGAGATCACCCCCATGGATGAAACTAACTTTAAGGAGTATTTACC
CGCACAATACCAGGGTTTCAACTCACTCAAGGAATTTAAAACTTACTTTCATAAGTTAGTTTCCGCTAAGAAACTGGAAA
TTACCCTACAAGAAAACAGTGTTAAAATTCGCCAGTTCTTTTTAGCTAACACCACCTTACCTTACATTCCTGATTCCTTA
ATTAAATTGGAATCCGATCGCTTACTGAGAGCGCAAAAGGATCAAGCGGAGCAGTACAAGATTCCCTTTGAGAGATTGTT
AGCAGCTTCCAAGCTTTCATTGGAGCAGTTGCAACAACGTAACATAAAAGAAGCACGTGATAACGTTACCTTTGCTTTAG
TGATGAAACGAATCGCTGATGTGGAAAAAATCAAGGTTGATAACAAAAAGATTCACAGCGAAATCGAATCGATTATTGAT
GTTGAATACCCCTTTGTTAATGCCGAGTTGAAAAAACAAATGTTCCACAACATGGAACAACAAAAGGACTTTGTGGAATC
GATAATTTTGAACCGTTTAACCACTACCAAGATAATTGAGTACTCCACTCATTAG

Upstream 100 bases:

>100_bases
AAAGGATGAACTGATCAAACTAATCGTTCATACGCTTTACGAACAGTTAGATCGTCCTGTTGGGGATGAAAATTAAAATA
TAGCTCAGATCTAGTTTAGA

Downstream 100 bases:

>100_bases
CACTCAAAGTATTAGAGTGCTAAAAAATGTGTTACAATTTAGCATTCTTCAACCTCTTATCTTAATAACTATGCCAGCTG
TAAAAAAACCACAAATTCTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKQYKLVNTTQKEKTLCLEIAIDTKLWKETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQ
PLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLV
DVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPV
EFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSL
IKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIID
VEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH

Sequences:

>Translated_444_residues
MKQYKLVNTTQKEKTLCLEIAIDTKL*KETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQ
PLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLV
DVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPV
EFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSL
IKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIID
VEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH
>Mature_444_residues
MKQYKLVNTTQKEKTLCLEIAIDTKL*KETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYLDRAELLQRSAQAVIDAIFQ
PLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVPQFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLV
DVSDKALANNDIAVIDFVGKVDGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPV
EFDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSVKIRQFFLANTTLPYIPDSL
IKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRNIKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIID
VEYPFVNAELKKQMFHNMEQQKDFVESIILNRLTTTKIIEYSTH

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=460, Percent_Identity=25, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 51150; Mature: 51150

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYKLVNTTQKEKTLCLEIAIDTKLKETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYL
CCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHH
DRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVP
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHCCCCEEEEECCCCEEEEEECCCCC
QFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKV
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHCCCCEEEEEEECCC
DGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVE
CCHHHHHCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHHCCCCEE
FDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSV
EEEEEEEEEEEECCCCCCCCHHHHCCHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCE
KIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRN
EEHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQ
HHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KDFVESIILNRLTTTKIIEYSTH
HHHHHHHHHHHHHHHHEEEECCC
>Mature Secondary Structure
MKQYKLVNTTQKEKTLCLEIAIDTKLKETQQKQTQDLTKNMKIKGFRKGKVPPTLAKDYL
CCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHH
DRAELLQRSAQAVIDAIFQPLQQEAVIADNENVIEDFPTIDFKTINENDCVILFDFDLVP
HHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCHHHHCCCCEEEEECCCCEEEEEECCCCC
QFEPPDYKHIKDLSPIVPLKDEEFNKELHNIEKNKGKLVDVSDKALANNDIAVIDFVGKV
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCHHHCCCCEEEEEEECCC
DGKVLESATAKQYELTIGSNSFIDGFESGLIGMKVGDKRQLKLKFPKDYHAEELKGKPVE
CCHHHHHCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEEEECCCCCCHHHHCCCCEE
FDIELKAIKQLEITPMDETNFKEYLPAQYQGFNSLKEFKTYFHKLVSAKKLEITLQENSV
EEEEEEEEEEEECCCCCCCCHHHHCCHHHCCHHHHHHHHHHHHHHHCCCEEEEEEECCCE
KIRQFFLANTTLPYIPDSLIKLESDRLLRAQKDQAEQYKIPFERLLAASKLSLEQLQQRN
EEHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IKEARDNVTFALVMKRIADVEKIKVDNKKIHSEIESIIDVEYPFVNAELKKQMFHNMEQQ
HHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
KDFVESIILNRLTTTKIIEYSTH
HHHHHHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 7984111 [H]