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The map label for this gene is mraW

Identifier: 13508054

GI number: 13508054

Start: 373829

End: 374755

Strand: Direct

Name: mraW

Synonym: MPN315

Alternate gene names: 13508054

Gene position: 373829-374755 (Clockwise)

Preceding gene: 13508053

Following gene: 13508055

Centisome position: 45.79

GC content: 40.02

Gene sequence:

>927_bases
ATGTTAAGTAATCAACCCCACAAAAGTGTCTTACTTGATGAAGTCATCCACAACCTCAACATTAAAGGTGATGGTAACTA
TTTAGATCTTACTGTTGGTTTTGGCGGTCATAGTCAGCACATTTTAGAGCAGCTTACCACTGGCACTTTAACTGGCAATG
ACATGGATAAGGACAGCATTACCTTTTGTACCGAGCTGTTCAAAGACAAAAAGAACGTTGTTTTAGTCCATGATAACTTC
GCTAACTTTTTTAACCACTTAAAGCAGCTCAAAGTAACGAAGTTTGACGGCATCTTAATTGATTTAGGGGTTTCGAGCTA
CCAGCTAGATAAACCAGAACGCGGTTTTAGTTTTAAACACGCCGGTCCATTTGACATGCGCATGCACCAAAGCGATCGCG
TACCAACTGCTTTAGATATCTTGGAGAGGTTGTCTGAGGAAGAATTGACACATGTTTTAAAGAAGTTTGGTGAAATAGCC
CACCCCAAACCAATCGCCAAGGCCTTAAAAACACTCATTAATAACACTAAAAATCCAACTACGGTAGAGGTAGCGGAAAC
AGTTAAGGCAGCTGCTAGCAACTTTGAAAAGTACAAATCACGCAATTACTTAGCTAAAGTTTTCCAAGCCATTCGCATTT
ACCTCAACCGTGAACTGGAAAGTTTGGAAATTGTGCTGCAACACATTCCCAAGCTGTTGAACAATAAGGGTCGTTTTCTA
GTGATTGTCTTCCATTCACTCGAGGAAAAATTGGTGCGGAACTACATTTTTAAACTTACTCACTTTGTCCAACCCCCTGA
ACTACCCGTTAAATTAACCCCACCGTTTGAACTCATTACTAAAAAACCCATTTTGCCAACTGAACAAGAAATTAAAACGA
ACCCCCGTGTTCGAAGTGCCAAACTGTTTGTCATTGAAAAGAAATAA

Upstream 100 bases:

>100_bases
ATTGGCCAGTTTGACCACTTAGAGATTTGGGACAAGAAGCTGTATGAGGACTACCTAGCCAACTCTGAGTCGTTGGAAAC
GGTAGCGGAAAGGATGAAAG

Downstream 100 bases:

>100_bases
CTATGTATAACCTCAAAAACATCTACGCCTCCATTACCCTTTACCCCCATGAAATTAACTTGGTGGTATCGGACAACAAC
AATCAGTTTCATGTCTTGTA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNF
ANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIA
HPKPIAKALKTLINNTKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFL
VIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSAKLFVIEKK

Sequences:

>Translated_308_residues
MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNF
ANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIA
HPKPIAKALKTLINNTKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFL
VIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSAKLFVIEKK
>Mature_308_residues
MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSITFCTELFKDKKNVVLVHDNF
ANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKHAGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIA
HPKPIAKALKTLINNTKNPTTVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFL
VIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSAKLFVIEKK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=330, Percent_Identity=32.7272727272727, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI165377202, Length=168, Percent_Identity=36.3095238095238, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1786270, Length=317, Percent_Identity=37.8548895899054, Blast_Score=187, Evalue=7e-49,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=31.306990881459, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=31.306990881459, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_MYCPN (P75466)

Other databases:

- EMBL:   U00089
- PIR:   S73847
- RefSeq:   NP_110003.1
- ProteinModelPortal:   P75466
- SMR:   P75466
- IntAct:   P75466
- GeneID:   876936
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN315
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   MPNE272634:MPN315-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35243; Mature: 35243

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 80-80 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSI
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHH
TFCTELFKDKKNVVLVHDNFANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKH
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEEECCEEEECCCCCEECCCCCCCCCCCC
AGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIAHPKPIAKALKTLINNTKNPT
CCCCCHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
TVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
VIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSA
EEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCCCCHHHHCCCCCEEEE
KLFVIEKK
EEEEEECC
>Mature Secondary Structure
MLSNQPHKSVLLDEVIHNLNIKGDGNYLDLTVGFGGHSQHILEQLTTGTLTGNDMDKDSI
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHH
TFCTELFKDKKNVVLVHDNFANFFNHLKQLKVTKFDGILIDLGVSSYQLDKPERGFSFKH
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEEECCEEEECCCCCEECCCCCCCCCCCC
AGPFDMRMHQSDRVPTALDILERLSEEELTHVLKKFGEIAHPKPIAKALKTLINNTKNPT
CCCCCHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
TVEVAETVKAAASNFEKYKSRNYLAKVFQAIRIYLNRELESLEIVLQHIPKLLNNKGRFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEE
VIVFHSLEEKLVRNYIFKLTHFVQPPELPVKLTPPFELITKKPILPTEQEIKTNPRVRSA
EEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCCCCCHHHHCCCCCEEEE
KLFVIEKK
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633