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The map label for this gene is lysS [H]

Identifier: 13508016

GI number: 13508016

Start: 326856

End: 328325

Strand: Direct

Name: lysS [H]

Synonym: MPN277

Alternate gene names: 13508016

Gene position: 326856-328325 (Clockwise)

Preceding gene: 13508014

Following gene: 13508017

Centisome position: 40.04

GC content: 42.18

Gene sequence:

>1470_bases
ATGAGCGATCGTTTAAATGATCAAGCACAAAATCGCTTGCAAAAACTCCTAAACCTCAAACAAACTGGCAACGATCCCTA
CCTCATTACTAAGATAGAAAACACCCACTCAGCGGCTAGTTTTCAAAAAGCATTTGCCAACCAATCTGACGCTGAGTTAA
AGCAGTACCAAGTGATCTTAACCGGTCGGATTATTGCGCTCCGGCAGACCTTTATTATTATCCAAGACTTTAGTGGGAAA
ATGCAGTTGTACATTAATAAAAAAACTGCCCCCGAACTGTTTGAATATTTCAATAATTACATTGATTTGGGTGATCAAAT
AGTAGCCACTGGCCATCCAATGATGACCAAAACAGGTGTTTTAACGCTGAACGTAGAGCGATTACAAATTGTCGCTAAAT
GCTTACAAACCCCACCTGAAAAATGACATGGGTTGACCGATCCAGAAGCGCGAGCACGAAAGCGTTACTTGGATTTGACC
TACAACCGTGCGCAGGTCGAAGTCTTTTTGAAACGCACCCAGATTATTACAGCTATCCGTACCTTTTTAAATGAAGCGGG
CTTTCTAGAAGTGGAAACACCCATTTTACAAGCCGTTTTAGGGGGTGCTAACGCCAAGCCCTTTAAAACACACTATAACG
CCTTAAAGGGTGATTTTTACTTGCGGATTGCAAATGAAATAGCACTCAAAAAGCTCATTGTGGGCGGTTTGCCGAAAGTG
TATGAAATGGGGCGGATGTTTCGTAACGAAGGGGTGGATACTACCCATAATCCGGAGTTTACGAGCATTGAAATGTATCA
GGCTAACGCTGACTATGCGGTCATGATGGATTTAACCGAAAACCTCATTAAGTTTGTTTGCAAAACACTCAATCAGTGAA
GTTTTAACTGGAACGGGCAAACACTTGATTTAGCCAAACCGTTCAAGAAAGTCAAAATGGTAGATTTAATCTGCCAAGTT
ACTGGGGTTAATTTTGATGATGTTAAGGATGATCAAACAGCCATTGCGCTTGCAAAGCAGCACAAAGTAGAGTTAAAAAA
GCACGAGCAGAACAAACAACACATTATTAACCGTTTCTTTGAACAGTTCTGTGAGCATACCTTAATACAACCCACCTTTG
TCACACACTATCCCAAGGCCGTTTCCCCGCTAGCTAAACAAGATCCCCACAACCCCGAGTTTACTGAGCGCTTTGAACTT
TTTATTAATACAAAAGAGTTAGCGAACGCTTACAGTGAGTTGAATGATCCCCTAGAACAACGTGCGCGCTTTGAACAACA
GTTGCAGGAAAAACGCATGGGTAATGATGAAGCAAGTGAATTAGATGAATCCTTTTTGGATGCTTTGAGTTTTGGTCTCC
CGCCAACAGGCGGCCTTGGGATTGGGGTTGATCGGTTAGTGATGTTATTGTGCGAGTGCAGTTCCATCCGTGACGTTGTT
TTCTTTCCCCAACTACGTGAATTGAAATAA

Upstream 100 bases:

>100_bases
ATAACTAGCACCCCGGCTACTGTTAGGATGACTACAAGATAGTTAAGCGACTGCATTAATTGCTTAAAATTAAGATAATA
ATTTTTTAAATTTGTCTTCG

Downstream 100 bases:

>100_bases
GTTTGATTTCATTGTTTTAGGTGCTGGCATTAGTGGCATAGTTTTATCACATGTTTTAGCACAGCATGGTAAAAGTGTTT
TACTGTTAGAAAAACGCAAC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGK
MQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGVLTLNVERLQIVAKCLQTPPEKWHGLTDPEARARKRYLDLT
YNRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKV
YEMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQWSFNWNGQTLDLAKPFKKVKMVDLICQV
TGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFEL
FINTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVV
FFPQLRELK

Sequences:

>Translated_489_residues
MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGK
MQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGVLTLNVERLQIVAKCLQTPPEK*HGLTDPEARARKRYLDLT
YNRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKV
YEMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQ*SFNWNGQTLDLAKPFKKVKMVDLICQV
TGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFEL
FINTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVV
FFPQLRELK
>Mature_488_residues
SDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVILTGRIIALRQTFIIIQDFSGKM
QLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGVLTLNVERLQIVAKCLQTPPEK*HGLTDPEARARKRYLDLTY
NRAQVEVFLKRTQIITAIRTFLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVY
EMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQ*SFNWNGQTLDLAKPFKKVKMVDLICQVT
GVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQFCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELF
INTKELANAYSELNDPLEQRARFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVF
FPQLRELK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=38.877755511022, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=38.877755511022, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI45439306, Length=346, Percent_Identity=25.4335260115607, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI40789249, Length=243, Percent_Identity=28.8065843621399, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789256, Length=499, Percent_Identity=41.2825651302605, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=40.4809619238477, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI87082379, Length=329, Percent_Identity=29.1793313069909, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788173, Length=224, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=497, Percent_Identity=38.6317907444668, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=497, Percent_Identity=38.6317907444668, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17535927, Length=497, Percent_Identity=38.6317907444668, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17551876, Length=345, Percent_Identity=23.1884057971014, Blast_Score=82, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=505, Percent_Identity=38.4158415841584, Blast_Score=332, Evalue=6e-92,
Organism=Saccharomyces cerevisiae, GI6324256, Length=474, Percent_Identity=33.3333333333333, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6325153, Length=208, Percent_Identity=25.9615384615385, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640849, Length=493, Percent_Identity=39.7565922920893, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24640851, Length=493, Percent_Identity=39.7565922920893, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24584738, Length=270, Percent_Identity=24.8148148148148, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55674; Mature: 55542

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVIL
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHEEEHH
TGRIIALRQTFIIIQDFSGKMQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGV
HHHHEEHHHEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCE
LTLNVERLQIVAKCLQTPPEKHGLTDPEARARKRYLDLTYNRAQVEVFLKRTQIITAIRT
EEEEHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVY
HHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCHHHH
EMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQSFNWNGQTL
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEE
DLAKPFKKVKMVDLICQVTGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELFINTKELANAYSELNDPLEQRA
HHHHHCCCCHHHHCCCHHHCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
RFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVFF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
PQLRELK
CCHHCCC
>Mature Secondary Structure 
SDRLNDQAQNRLQKLLNLKQTGNDPYLITKIENTHSAASFQKAFANQSDAELKQYQVIL
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHEEEHH
TGRIIALRQTFIIIQDFSGKMQLYINKKTAPELFEYFNNYIDLGDQIVATGHPMMTKTGV
HHHHEEHHHEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCE
LTLNVERLQIVAKCLQTPPEKHGLTDPEARARKRYLDLTYNRAQVEVFLKRTQIITAIRT
EEEEHHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FLNEAGFLEVETPILQAVLGGANAKPFKTHYNALKGDFYLRIANEIALKKLIVGGLPKVY
HHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHCCCHHHH
EMGRMFRNEGVDTTHNPEFTSIEMYQANADYAVMMDLTENLIKFVCKTLNQSFNWNGQTL
HHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCEE
DLAKPFKKVKMVDLICQVTGVNFDDVKDDQTAIALAKQHKVELKKHEQNKQHIINRFFEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FCEHTLIQPTFVTHYPKAVSPLAKQDPHNPEFTERFELFINTKELANAYSELNDPLEQRA
HHHHHCCCCHHHHCCCHHHCCHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
RFEQQLQEKRMGNDEASELDESFLDALSFGLPPTGGLGIGVDRLVMLLCECSSIRDVVFF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEC
PQLRELK
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]