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The map label for this gene is ppnK

Identifier: 13508006

GI number: 13508006

Start: 318581

End: 319360

Strand: Direct

Name: ppnK

Synonym: MPN267

Alternate gene names: 13508006

Gene position: 318581-319360 (Clockwise)

Preceding gene: 13508005

Following gene: 13508007

Centisome position: 39.02

GC content: 40.13

Gene sequence:

>780_bases
ATGAAGTACAAGATTTTTGCATCAACAACACCACAAACCGAGCCAGTTTTGCAAAAGTTAAAGCAGGTGTTGAAAGGTTG
TGAGGCAGTTGAAAAGGGCTTTGATTATCTCTTTGTATTAGGTGGTGATGGTTTCTTTGTTTCTACTGTGGCCAACTATA
ACTGCCACAATTGTAGGGTAGTAGGGATTAACACTGGTCATCTAGGTTTTTACACATCGTTTAATGAAAAGGATTTGGAT
GATAATTTCCTCCAAAAGCTCCAGCAATGTCACTTTCAACGGATTAGTTTGTTGGAAGTTAGTGTCAATGGCCAGCAGCA
CTTGGTTTTGAATGAACTGGCAGTTTACACCAACACAGCTTACCCAATTAACATCTTTATTGATGGTGAAGCGTGGGAGT
TTTACCGCGGTTCAGGTTTATTAATCGGTCCGCGTACTGGTTCGACTGCACTGGCTAAATCTGCTAAAGGGGCGGTGATT
TTCCCTGGCATTGATGTGCTGCAAATTATTGAAATGAATCCACTTTTGCACCCCAATCAAGTTACTATCCAATCACCAAT
TATTTTGCCCAAAGAAACGCAAGTGGAATTTGTGGTCAAAAAAGCCTTTAACCCACAACAGTTTCCCACCTTTTACTGTG
ACGGGAGAAAGTTGGAATTGCCAAACGCTGACACAACTTTAGCGCTTAAACTAGTGCAATCCACACCGATGTTTAACATT
TCACTCAAAACACAGGACTTTATCAATAAACTTAAGTCCACTTTTATTAAACAAAGCTAA

Upstream 100 bases:

>100_bases
CGGGGATTCCCAAGCGGATGCGCATTGGTTATAACGCCGCTGAAATTAAGGTATTTGAACGCAGCTTAATGGAACCTAAA
GTGCGTACAGTAAAGAAGTA

Downstream 100 bases:

>100_bases
TGAAAGTATTGTTGTGAATTGGTTATGTGTTGAGCTTTGGCTTACTTTACCTTTATTTGGTAAAACGTGCTAAGAGGGCC
GCGCTACAATTAAACAACAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLD
DNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVI
FPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNI
SLKTQDFINKLKSTFIKQS

Sequences:

>Translated_259_residues
MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLD
DNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVI
FPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNI
SLKTQDFINKLKSTFIKQS
>Mature_259_residues
MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRVVGINTGHLGFYTSFNEKDLD
DNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTAYPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVI
FPGIDVLQIIEMNPLLHPNQVTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNI
SLKTQDFINKLKSTFIKQS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=172, Percent_Identity=33.1395348837209, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_MYCPN (P75508)

Other databases:

- EMBL:   U00089
- PIR:   S73892
- RefSeq:   NP_109955.1
- ProteinModelPortal:   P75508
- SMR:   P75508
- IntAct:   P75508
- GeneID:   877097
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN267
- HOGENOM:   HBG130030
- OMA:   YINNEFL
- ProtClustDB:   PRK00561
- BioCyc:   MPNE272634:MPN267-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 29034; Mature: 29034

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEE
VGINTGHLGFYTSFNEKDLDDNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTA
EEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEEEEECCCEEEEEEEEEEEECCC
YPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVIFPGIDVLQIIEMNPLLHPNQ
EEEEEEECCCCEEEECCCEEEEECCCCCHHHHHCCCCEEEECCCCEEEEEECCCCCCCCE
VTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNI
EEEECCEECCCCCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCEEEE
SLKTQDFINKLKSTFIKQS
EECHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYKIFASTTPQTEPVLQKLKQVLKGCEAVEKGFDYLFVLGGDGFFVSTVANYNCHNCRV
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCEEE
VGINTGHLGFYTSFNEKDLDDNFLQKLQQCHFQRISLLEVSVNGQQHLVLNELAVYTNTA
EEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEEEEECCCEEEEEEEEEEEECCC
YPINIFIDGEAWEFYRGSGLLIGPRTGSTALAKSAKGAVIFPGIDVLQIIEMNPLLHPNQ
EEEEEEECCCCEEEECCCEEEEECCCCCHHHHHCCCCEEEECCCCEEEEEECCCCCCCCE
VTIQSPIILPKETQVEFVVKKAFNPQQFPTFYCDGRKLELPNADTTLALKLVQSTPMFNI
EEEECCEECCCCCCCEEEEECCCCCCCCCEEEECCEEEECCCCCCEEEEEEECCCCEEEE
SLKTQDFINKLKSTFIKQS
EECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633