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The map label for this gene is rpoA [H]

Identifier: 13507930

GI number: 13507930

Start: 231448

End: 232431

Strand: Direct

Name: rpoA [H]

Synonym: MPN191

Alternate gene names: 13507930

Gene position: 231448-232431 (Clockwise)

Preceding gene: 13507929

Following gene: 13507931

Centisome position: 28.35

GC content: 43.39

Gene sequence:

>984_bases
ATGGAAAAGTTTTTAAAGTACGAAATAAAGGTAAATAACGAACAAGCACGCGCTAACCCCAACTACGGCATCTTTGAAGT
TGGACCACTCGAATCGGGCTTTGTCATTACCATCGGTAATGCTATGCGCCGGGTGCTGTTGAGCTGTATTCCAGGTGCCA
GTGTGTTTGCACTCTCAATTTCGGGTGCCAAGCAAGAGTTTGCTGCTGTGGAAGGGATGAAGGAAGATGTCACTGAAGTG
GTGTTAAACTTCAAGCAATTGGTAGTGAAGATTTCTGACCTTCTGTTTGAAGATGGTGAAATGGTCGAACCACCACTAGA
ACGTTGACCATTGTTAACGGTAACAGCTGAGAAAGCGGGACCGGTGTATGCGAAGGACTTGGAGTGCCCAGCTGGCTTTG
AAGTGGTCAACAAGGACTTGTACCTGTTCTCGTTGCAAACTGACAAAAAGGTAACAGTTAACGTGTATGTGAAACAAGGA
CGGGGGTTTGTTACTTTCTTGGAAAACCGTGAAATGATTAATTCTTTGGGGATTATTGCCACTGACTCGAACTTCTCCCC
GGTATTACACTGTGGTTATGAAGTACAGGAATTGAAAACTTCCAAGCAAAAGATTACTGATCACTTAACTTTCAAAATTG
CTACTAACGGTGCCATATCCGCTGTGGATGCCTTTGCAATGGCTGCAAAGATCTTGATCGAACACCTCAATCCGATTGTT
AATGTCAACGAATCGATTAAGGCGTTGAATATTATCCAAGAGAAAGCCGAAGAACGCCGTGTCCGTTCGTTTGCGAAGCA
AATTGAAGAGTTGGACTTTACCGTGCGTACCTTTAACTGTTTGAAGCGTAGTGGGATCCACACCTTACAAGAGTTGTTGT
CCAAGTCATTAGCTGATATTCGTGAAATCCGTAACCTCGGTAAGAAATCGGAACGCGAAATTATTAAGAAGGTACACGAA
TTAGGACTCAAATTAAGATCTTAG

Upstream 100 bases:

>100_bases
GCAGCTTTGCTAATGCGGGACTTTCTATTACTGAAATTAACGAAAAGACCCCGATTCCCCACAACGGTTGCAAACCACCA
AAACGACCACGCTAAGATTT

Downstream 100 bases:

>100_bases
TTATGTCATACATTAATAAACCAGGTAAAACTAGTGCGTGAAGAGTCATGACCGTGCGCCAACAGGTAAGCGCTGTTTTG
GCTTATGGCAAGATTGAAAC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEV
VLNFKQLVVKISDLLFEDGEMVEPPLERWPLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQG
RGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIV
NVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHE
LGLKLRS

Sequences:

>Translated_327_residues
MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEV
VLNFKQLVVKISDLLFEDGEMVEPPLER*PLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQG
RGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIV
NVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHE
LGLKLRS
>Mature_327_residues
MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSISGAKQEFAAVEGMKEDVTEV
VLNFKQLVVKISDLLFEDGEMVEPPLER*PLLTVTAEKAGPVYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQG
RGFVTFLENREMINSLGIIATDSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIV
NVNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIREIRNLGKKSEREIIKKVHE
LGLKLRS

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=301, Percent_Identity=31.8936877076412, Blast_Score=147, Evalue=6e-37,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36458; Mature: 36458

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSI
CCCCEEEEEEECCCHHCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEE
SGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLERPLLTVTAEKAGP
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
VYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIAT
CEECCCCCCCCHHHHCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCHHHHHHCCEEEE
DSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVN
CCCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHCC
VNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EIRNLGKKSEREIIKKVHELGLKLRS
HHHHCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKFLKYEIKVNNEQARANPNYGIFEVGPLESGFVITIGNAMRRVLLSCIPGASVFALSI
CCCCEEEEEEECCCHHCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHCCCCEEEEEEE
SGAKQEFAAVEGMKEDVTEVVLNFKQLVVKISDLLFEDGEMVEPPLERPLLTVTAEKAGP
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCC
VYAKDLECPAGFEVVNKDLYLFSLQTDKKVTVNVYVKQGRGFVTFLENREMINSLGIIAT
CEECCCCCCCCHHHHCCCEEEEEECCCCEEEEEEEEECCCCEEEEECCHHHHHHCCEEEE
DSNFSPVLHCGYEVQELKTSKQKITDHLTFKIATNGAISAVDAFAMAAKILIEHLNPIVN
CCCCCCCEECCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHCC
VNESIKALNIIQEKAEERRVRSFAKQIEELDFTVRTFNCLKRSGIHTLQELLSKSLADIR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
EIRNLGKKSEREIIKKVHELGLKLRS
HHHHCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8604303; 8948633 [H]