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The map label for this gene is pinE [C]

Identifier: 13488190

GI number: 13488190

Start: 25897

End: 26766

Strand: Direct

Name: pinE [C]

Synonym: mlr9024

Alternate gene names: 13488190

Gene position: 25897-26766 (Clockwise)

Preceding gene: 13488189

Following gene: 13488191

Centisome position: 7.36

GC content: 58.85

Gene sequence:

>870_bases
ATGACCCGCGTCGGATATGCCCGCGTCAGCACAACGGACCAGGATCTCGATATCCAAGTCGCCAGGTTGAAGGCCGCTGG
CTGTGAGATCGTTCGTTCGGAAACGGGATCGGGCGCATCACGAACCGGGCGCACTGAGCTTGAGACGATCATGCAGTTCT
TGCGCGACGGCGACGAGCTTGTCGTCTTGCGTCTCGATCGGCTCGGCCGCTCCACACGCGATGTTCTGAACCTGGTGCAT
GAACTCGAAGAAAGAGGAGCCTCGCTGCGGGTGCTTGAGCCGGAGGTCACAACGGCCGGAAGCATGGGAAGGATGGTGAT
CACCATCCTCGGCATGGTCGCCGACATGGAACTGAAGTTCATCAAGGATCGCCAGCGCGCCGGGATCGCAGCGGCACGAG
CCGACGGCGTCTACAAAGGCCGAAAGAAAAACGTCGATGATGACGAGATTCGACGCCGTGTTGCCGCCGGCGCCAGCAAG
GCCAGCGTCGCGCGTGATCTGAAGGTCTCAAGAATGACCGTTTACCGGGCCCTCGACATTGTTCCGCAAAGGACAGCCTC
ACTCCCCGAAAAGCCGCCTGCCGCCAGTATCTCCTTGCACCTGATCATCGAGAACTTCAACAAACACGGTCGCGGCAGGA
AGCCCGCTCGCGAACGGATCGAGGAAATGCTGGAGCGCGAATACGCCATGGTGAAAACCGGCAATTGCGACTACAGGCTG
ACGGTTGTCTACGACCGCGATCCGGAAGGTTCTGATCTCGATAGTGAAATCCAGCATCTTTATTCCGAAATGTTCAATAT
CGCCGAGAGCCACCGTTGCTCAATCGAGGCTGACATCCATGAGATCGGCGGAGAGGGGCGAAGCTGGTAG

Upstream 100 bases:

>100_bases
CGATCGAAAGTGACGTATGCTGCATGTCACAAAAGGGTGGATTCGTCAGCAATGTGACGATACAAGGAAGAAGCCACCCT
AATGTGACGGATTCGCCCGT

Downstream 100 bases:

>100_bases
GGACGTTCGTTTTTTGTTCTTCACTTGGCCAAAATCGCAGCTTCGGGCCGACAGGGCCAAGTAGGCGCTCCAGGTTAGAA
AAGCTGCCGGTATATCCACG

Product: resolvase/integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTRVGYARVSTTDQDLDIQVARLKAAGCEIVRSETGSGASRTGRTELETIMQFLRDGDELVVLRLDRLGRSTRDVLNLVH
ELEERGASLRVLEPEVTTAGSMGRMVITILGMVADMELKFIKDRQRAGIAAARADGVYKGRKKNVDDDEIRRRVAAGASK
ASVARDLKVSRMTVYRALDIVPQRTASLPEKPPAASISLHLIIENFNKHGRGRKPARERIEEMLEREYAMVKTGNCDYRL
TVVYDRDPEGSDLDSEIQHLYSEMFNIAESHRCSIEADIHEIGGEGRSW

Sequences:

>Translated_289_residues
MTRVGYARVSTTDQDLDIQVARLKAAGCEIVRSETGSGASRTGRTELETIMQFLRDGDELVVLRLDRLGRSTRDVLNLVH
ELEERGASLRVLEPEVTTAGSMGRMVITILGMVADMELKFIKDRQRAGIAAARADGVYKGRKKNVDDDEIRRRVAAGASK
ASVARDLKVSRMTVYRALDIVPQRTASLPEKPPAASISLHLIIENFNKHGRGRKPARERIEEMLEREYAMVKTGNCDYRL
TVVYDRDPEGSDLDSEIQHLYSEMFNIAESHRCSIEADIHEIGGEGRSW
>Mature_288_residues
TRVGYARVSTTDQDLDIQVARLKAAGCEIVRSETGSGASRTGRTELETIMQFLRDGDELVVLRLDRLGRSTRDVLNLVHE
LEERGASLRVLEPEVTTAGSMGRMVITILGMVADMELKFIKDRQRAGIAAARADGVYKGRKKNVDDDEIRRRVAAGASKA
SVARDLKVSRMTVYRALDIVPQRTASLPEKPPAASISLHLIIENFNKHGRGRKPARERIEEMLEREYAMVKTGNCDYRLT
VVYDRDPEGSDLDSEIQHLYSEMFNIAESHRCSIEADIHEIGGEGRSW

Specific function: This Protein Catalyzes The Inversion Of An 1800-Bp E.Coli DNA Fragment, The P Region, Which Can Exist In Either Orientation. The Function Of The Inversion Is Not Yet Clear. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=176, Percent_Identity=39.2045454545455, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787638, Length=187, Percent_Identity=28.8770053475936, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 32343; Mature: 32212

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVGYARVSTTDQDLDIQVARLKAAGCEIVRSETGSGASRTGRTELETIMQFLRDGDEL
CCCCCCEEECCCCCCCCCEEEHHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEE
VVLRLDRLGRSTRDVLNLVHELEERGASLRVLEPEVTTAGSMGRMVITILGMVADMELKF
EEEEEHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
IKDRQRAGIAAARADGVYKGRKKNVDDDEIRRRVAAGASKASVARDLKVSRMTVYRALDI
HHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VPQRTASLPEKPPAASISLHLIIENFNKHGRGRKPARERIEEMLEREYAMVKTGNCDYRL
CCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEE
TVVYDRDPEGSDLDSEIQHLYSEMFNIAESHRCSIEADIHEIGGEGRSW
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCC
>Mature Secondary Structure 
TRVGYARVSTTDQDLDIQVARLKAAGCEIVRSETGSGASRTGRTELETIMQFLRDGDEL
CCCCCEEECCCCCCCCCEEEHHHHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEE
VVLRLDRLGRSTRDVLNLVHELEERGASLRVLEPEVTTAGSMGRMVITILGMVADMELKF
EEEEEHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
IKDRQRAGIAAARADGVYKGRKKNVDDDEIRRRVAAGASKASVARDLKVSRMTVYRALDI
HHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
VPQRTASLPEKPPAASISLHLIIENFNKHGRGRKPARERIEEMLEREYAMVKTGNCDYRL
CCHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEE
TVVYDRDPEGSDLDSEIQHLYSEMFNIAESHRCSIEADIHEIGGEGRSW
EEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]