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The map label for this gene is gatA [H]

Identifier: 13488186

GI number: 13488186

Start: 17495

End: 18895

Strand: Direct

Name: gatA [H]

Synonym: mlr9017

Alternate gene names: 13488186

Gene position: 17495-18895 (Clockwise)

Preceding gene: 13488185

Following gene: 13488187

Centisome position: 4.97

GC content: 61.38

Gene sequence:

>1401_bases
ATGATATTTGACCCGTTCTGGTCAATCGAGCAACTATCGGAACGCTTCGCTCGGCGGGAACTGTCGTCCGTGGAAGCTGT
AACGGAGCTCGTCAGGCGCATAGCGGCATTGGATGGCCGGCTGCACGCCTTTTGTCAGGTCGATGAGGACGGCGCGCTGG
CACGCGCCCGAGCCGCGGACGAAGCACGAGCTAACGGAAATTCCACGTCCCCTCTGCTCGGCGTGCCGTTAGCCGTGAAG
GACATCTTCGATCAGGCCGGGCATGGCACGCATGCTGGCTCCAACGCTCTGAATGACAGGCTGCCAATCCGATCGGCAAA
TGCCGTCGAAAAGCTCGAATCCGCAGGCATGATCGCCATCGGCCGCACCAACATGGTTGAGTTCGCCTATGGCGGATGGG
GAACAAACCAAGTTCAGGGCGCGCCGTGCAATCCCTGGGGTCTGGACACTCATGTCGTTGCAGGCGGCTCAAGCAGCGGC
TCGGCAGTTGCGGTCGCTGGTGGATTCGCCCCAGCCGCTTTGGGTACCGATACTGGCGGCTCGATCCGCACGCCTGCCGC
CTGGTGCGGAATCGTCGGCCTCAAGACTTCAGTCGGGCTAGTCGGGCGCGGCGGTGTCGTGCCGCTATGCCCGACACATG
ACAGCGTCGGACCGATGACCCGTACCGTCCGTGACGCCGCGCTTCTGTTGGAAGCTATGGTGGGGTCGGATCCTCGCGAT
GTCGCCACGTGGCACGTTCCCTCCGTGCGTTCCCTTACCACGATCGAGGACGGCGTGAAGGGCCTGCGGATCGGAATGCT
CCACGAGCGGGATTTGGCAGGCGTCGAGCCCGACATCCGGATGCTGCATGATCGCGCAATCGCCGATCTGACAGCTCTTG
GCGCCCAATTCCACAAAATCGCTCTGCCATTGTCCATCAGCGATTATCTCGGCCGTGGCGGCGACATTATGAGCGTCGAG
AGCTATGCCTTTCTCAGCGCTTATGTCGAACATCCGGACAGCCCGGTGGATCCGGTCATTGCAGAACGCATCAGCCGCGG
CCGCGACATCAGCGGACCGGCTTACTACCGCCTTCTCGAAACCCGCCGGCTGGCGCAGATCGGCTTTCAGGAAGCGGCAG
CAGGATTTGACGCGTTCGTCGCGCCTGGCTCGCATCGAAGTCCAGTACCGCTTGGCGAGGTAGACGAAAGCCAGCCTCCC
AATCATTTTGGAAGACTGGTGAACTATCTCGATCTCGCCAGCCTAGTAGTCCCGGTCGGATTGACATCAAATGGCCTGCC
AGCTGGCCTGCAGATTGTTGTCAGAAAATTCGACGATGCCTTGGCACTTCGCATCGGACGGACGTTGGAACGTGAGCGTG
GTGGCCTGTTTGTGCCTGCGCCGGGGTTGCTGGCCCAATGA

Upstream 100 bases:

>100_bases
CGCTCATCGTCGTCGTCAACGTAGCGGCAGTCTTGGTCGTTGACGCGGTGATCGGAAGTCGCGGATCGGCCGCCTAGGAG
CACACCAGAGGATTTGTTCA

Downstream 100 bases:

>100_bases
AGTCCATGGCTGATTTCCACAACCTGGCGAGCTCTCTCATTTACCCATGCCAATACCGGGCCCAACCGGATTGCGAGGGC
TTTTGAAAACACTGCAGGTG

Product: Glu-tRNA(Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MIFDPFWSIEQLSERFARRELSSVEAVTELVRRIAALDGRLHAFCQVDEDGALARARAADEARANGNSTSPLLGVPLAVK
DIFDQAGHGTHAGSNALNDRLPIRSANAVEKLESAGMIAIGRTNMVEFAYGGWGTNQVQGAPCNPWGLDTHVVAGGSSSG
SAVAVAGGFAPAALGTDTGGSIRTPAAWCGIVGLKTSVGLVGRGGVVPLCPTHDSVGPMTRTVRDAALLLEAMVGSDPRD
VATWHVPSVRSLTTIEDGVKGLRIGMLHERDLAGVEPDIRMLHDRAIADLTALGAQFHKIALPLSISDYLGRGGDIMSVE
SYAFLSAYVEHPDSPVDPVIAERISRGRDISGPAYYRLLETRRLAQIGFQEAAAGFDAFVAPGSHRSPVPLGEVDESQPP
NHFGRLVNYLDLASLVVPVGLTSNGLPAGLQIVVRKFDDALALRIGRTLERERGGLFVPAPGLLAQ

Sequences:

>Translated_466_residues
MIFDPFWSIEQLSERFARRELSSVEAVTELVRRIAALDGRLHAFCQVDEDGALARARAADEARANGNSTSPLLGVPLAVK
DIFDQAGHGTHAGSNALNDRLPIRSANAVEKLESAGMIAIGRTNMVEFAYGGWGTNQVQGAPCNPWGLDTHVVAGGSSSG
SAVAVAGGFAPAALGTDTGGSIRTPAAWCGIVGLKTSVGLVGRGGVVPLCPTHDSVGPMTRTVRDAALLLEAMVGSDPRD
VATWHVPSVRSLTTIEDGVKGLRIGMLHERDLAGVEPDIRMLHDRAIADLTALGAQFHKIALPLSISDYLGRGGDIMSVE
SYAFLSAYVEHPDSPVDPVIAERISRGRDISGPAYYRLLETRRLAQIGFQEAAAGFDAFVAPGSHRSPVPLGEVDESQPP
NHFGRLVNYLDLASLVVPVGLTSNGLPAGLQIVVRKFDDALALRIGRTLERERGGLFVPAPGLLAQ
>Mature_466_residues
MIFDPFWSIEQLSERFARRELSSVEAVTELVRRIAALDGRLHAFCQVDEDGALARARAADEARANGNSTSPLLGVPLAVK
DIFDQAGHGTHAGSNALNDRLPIRSANAVEKLESAGMIAIGRTNMVEFAYGGWGTNQVQGAPCNPWGLDTHVVAGGSSSG
SAVAVAGGFAPAALGTDTGGSIRTPAAWCGIVGLKTSVGLVGRGGVVPLCPTHDSVGPMTRTVRDAALLLEAMVGSDPRD
VATWHVPSVRSLTTIEDGVKGLRIGMLHERDLAGVEPDIRMLHDRAIADLTALGAQFHKIALPLSISDYLGRGGDIMSVE
SYAFLSAYVEHPDSPVDPVIAERISRGRDISGPAYYRLLETRRLAQIGFQEAAAGFDAFVAPGSHRSPVPLGEVDESQPP
NHFGRLVNYLDLASLVVPVGLTSNGLPAGLQIVVRKFDDALALRIGRTLERERGGLFVPAPGLLAQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=478, Percent_Identity=32.2175732217573, Blast_Score=176, Evalue=5e-44,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=27.0491803278689, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=27.9176201372998, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=301, Percent_Identity=26.2458471760797, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=486, Percent_Identity=23.8683127572016, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=262, Percent_Identity=26.3358778625954, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=305, Percent_Identity=26.5573770491803, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=336, Percent_Identity=26.4880952380952, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556276, Length=328, Percent_Identity=26.8292682926829, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=394, Percent_Identity=30.2030456852792, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323950, Length=281, Percent_Identity=25.2669039145907, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648113, Length=491, Percent_Identity=28.5132382892057, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=27.6209677419355, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=27.6209677419355, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=27.6209677419355, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=27.6209677419355, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24644968, Length=491, Percent_Identity=24.847250509165, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=27.4, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356731, Length=498, Percent_Identity=25.7028112449799, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648435, Length=483, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648437, Length=483, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45550774, Length=483, Percent_Identity=26.0869565217391, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648441, Length=418, Percent_Identity=26.0765550239234, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648439, Length=418, Percent_Identity=26.0765550239234, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49203; Mature: 49203

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDPFWSIEQLSERFARRELSSVEAVTELVRRIAALDGRLHAFCQVDEDGALARARAAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
EARANGNSTSPLLGVPLAVKDIFDQAGHGTHAGSNALNDRLPIRSANAVEKLESAGMIAI
HHHCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEE
GRTNMVEFAYGGWGTNQVQGAPCNPWGLDTHVVAGGSSSGSAVAVAGGFAPAALGTDTGG
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHCCCCCCC
SIRTPAAWCGIVGLKTSVGLVGRGGVVPLCPTHDSVGPMTRTVRDAALLLEAMVGSDPRD
CCCCCHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VATWHVPSVRSLTTIEDGVKGLRIGMLHERDLAGVEPDIRMLHDRAIADLTALGAQFHKI
CEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
ALPLSISDYLGRGGDIMSVESYAFLSAYVEHPDSPVDPVIAERISRGRDISGPAYYRLLE
EECEEHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHH
TRRLAQIGFQEAAAGFDAFVAPGSHRSPVPLGEVDESQPPNHFGRLVNYLDLASLVVPVG
HHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
LTSNGLPAGLQIVVRKFDDALALRIGRTLERERGGLFVPAPGLLAQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHCCC
>Mature Secondary Structure
MIFDPFWSIEQLSERFARRELSSVEAVTELVRRIAALDGRLHAFCQVDEDGALARARAAD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHH
EARANGNSTSPLLGVPLAVKDIFDQAGHGTHAGSNALNDRLPIRSANAVEKLESAGMIAI
HHHCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEE
GRTNMVEFAYGGWGTNQVQGAPCNPWGLDTHVVAGGSSSGSAVAVAGGFAPAALGTDTGG
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCHHCCCCCCC
SIRTPAAWCGIVGLKTSVGLVGRGGVVPLCPTHDSVGPMTRTVRDAALLLEAMVGSDPRD
CCCCCHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VATWHVPSVRSLTTIEDGVKGLRIGMLHERDLAGVEPDIRMLHDRAIADLTALGAQFHKI
CEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHE
ALPLSISDYLGRGGDIMSVESYAFLSAYVEHPDSPVDPVIAERISRGRDISGPAYYRLLE
EECEEHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHH
TRRLAQIGFQEAAAGFDAFVAPGSHRSPVPLGEVDESQPPNHFGRLVNYLDLASLVVPVG
HHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
LTSNGLPAGLQIVVRKFDDALALRIGRTLERERGGLFVPAPGLLAQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA