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The map label for this gene is galE [C]

Identifier: 13488052

GI number: 13488052

Start: 6884

End: 7933

Strand: Reverse

Name: galE [C]

Synonym: mll9007

Alternate gene names: 13488052

Gene position: 7933-6884 (Counterclockwise)

Preceding gene: 13488053

Following gene: 13488179

Centisome position: 2.25

GC content: 50.19

Gene sequence:

>1050_bases
ATGGTGAATACAGCTCTAGTTACCGGCGGTTCGGGATATTTTGGGGAATTGTTGACCAAACAGTTACTTGAGCAAGGAAC
CCACGTTCGCGTGTTCGACCTAAATTTCCCTGGCTTCAGTCATCCTAATCTGGAATTTTTCAAAGGCACGATTCTGGACC
GTAACGCGGTCAAACAAGCAGTCTCCGGCGTCAAAAAGGTGTTCCACAACGTTGCCCAAGTGCCTTTAGCCAAGGATGTG
GACCTATTCTGGTCCATCAACAAGGACGGTACTCAGATCCTTGCCGACGAATCGGTTGCGGCAGGAGTCGTGAAGCTGAT
CTATACTTCTTCCAGCGCTGTTTTTGGCGTCCCCAAAGCGAATCCCGTCACAGAAAATACAGTGACCAATCCTGCTGAAG
ACTACGGACGCGCAAAATTGGCAGGAGAAATGATTTGCAAGGAGGCAATGCAACGCCATGGTCTGGATGTGGCGATTGTG
CGCCCGCGGACCATTCTCGGACACGGCCGCCTTGGCATCGTCCAAATTCTCTTTGACTGGATAGAACGGGGCCTCGACAT
TCCAGTTTTTGCTGGCGGGAATAACAGATATCAGTTCGTTCACTCGGATGATTTGGCCTGGGCATGCATTGCAGCATCGG
ATATGCGGGGATTTGCGGAATACAATATTGGGGCGGCCGAATTTGGCACAATGCGTGAACTTCTGCAGACGGTAATTGAC
CATACTGGAACCGAAAGCCGTATCAAGTCTATTCCAATGAGGCCGACTGCCCTTGCAGCGAATTTAGCAAGTACGCTCGG
GCTTTCTCCCTTGGGTCCCTACCATTCGCTTATGTACGGGCGCTCGATGTATTTCGATATTTCGAAAGCACAGAGAGAAC
TTGATTTTATGCCGAAGTATTCTAATTCTCAGATGATCATCGAAACTTATGATTGGTATCAGGCCAATAAGGCCTCTCTG
AGCAAAGGCAGAGCGTCACGCCATAAGTCGCCGGTCAAGCAGCAAATTCTGGCACTGGTTCCTTACGTATTGCGGATGGC
CCCGGGATGA

Upstream 100 bases:

>100_bases
TTTGCACTGAACCGCATGATAGCGGTCCGCACGAACGAAAGGCTGACAAGTGGTCTCAGCAGTTTCTTGAAATTGGGTTT
CAGCTTTTTGTAGGTCAGAG

Downstream 100 bases:

>100_bases
ATACGCCACCTGTGATCATGGTTCTTGCTGCGGGTTTGAATTCCTGTATCGGCAATATGTTGCTGAAATGGAGCAGGATC
TCCCTGCCTACCGACGCTGG

Product: hypothetical protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MVNTALVTGGSGYFGELLTKQLLEQGTHVRVFDLNFPGFSHPNLEFFKGTILDRNAVKQAVSGVKKVFHNVAQVPLAKDV
DLFWSINKDGTQILADESVAAGVVKLIYTSSSAVFGVPKANPVTENTVTNPAEDYGRAKLAGEMICKEAMQRHGLDVAIV
RPRTILGHGRLGIVQILFDWIERGLDIPVFAGGNNRYQFVHSDDLAWACIAASDMRGFAEYNIGAAEFGTMRELLQTVID
HTGTESRIKSIPMRPTALAANLASTLGLSPLGPYHSLMYGRSMYFDISKAQRELDFMPKYSNSQMIIETYDWYQANKASL
SKGRASRHKSPVKQQILALVPYVLRMAPG

Sequences:

>Translated_349_residues
MVNTALVTGGSGYFGELLTKQLLEQGTHVRVFDLNFPGFSHPNLEFFKGTILDRNAVKQAVSGVKKVFHNVAQVPLAKDV
DLFWSINKDGTQILADESVAAGVVKLIYTSSSAVFGVPKANPVTENTVTNPAEDYGRAKLAGEMICKEAMQRHGLDVAIV
RPRTILGHGRLGIVQILFDWIERGLDIPVFAGGNNRYQFVHSDDLAWACIAASDMRGFAEYNIGAAEFGTMRELLQTVID
HTGTESRIKSIPMRPTALAANLASTLGLSPLGPYHSLMYGRSMYFDISKAQRELDFMPKYSNSQMIIETYDWYQANKASL
SKGRASRHKSPVKQQILALVPYVLRMAPG
>Mature_349_residues
MVNTALVTGGSGYFGELLTKQLLEQGTHVRVFDLNFPGFSHPNLEFFKGTILDRNAVKQAVSGVKKVFHNVAQVPLAKDV
DLFWSINKDGTQILADESVAAGVVKLIYTSSSAVFGVPKANPVTENTVTNPAEDYGRAKLAGEMICKEAMQRHGLDVAIV
RPRTILGHGRLGIVQILFDWIERGLDIPVFAGGNNRYQFVHSDDLAWACIAASDMRGFAEYNIGAAEFGTMRELLQTVID
HTGTESRIKSIPMRPTALAANLASTLGLSPLGPYHSLMYGRSMYFDISKAQRELDFMPKYSNSQMIIETYDWYQANKASL
SKGRASRHKSPVKQQILALVPYVLRMAPG

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI193211614, Length=214, Percent_Identity=32.2429906542056, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI8393516, Length=214, Percent_Identity=32.2429906542056, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI116268111, Length=356, Percent_Identity=26.123595505618, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38415; Mature: 38415

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNTALVTGGSGYFGELLTKQLLEQGTHVRVFDLNFPGFSHPNLEFFKGTILDRNAVKQA
CCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHH
VSGVKKVFHNVAQVPLAKDVDLFWSINKDGTQILADESVAAGVVKLIYTSSSAVFGVPKA
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHEEECCCCEEECCCC
NPVTENTVTNPAEDYGRAKLAGEMICKEAMQRHGLDVAIVRPRTILGHGRLGIVQILFDW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHH
IERGLDIPVFAGGNNRYQFVHSDDLAWACIAASDMRGFAEYNIGAAEFGTMRELLQTVID
HHCCCCCEEEECCCCEEEEEECCCCEEEEEEHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
HTGTESRIKSIPMRPTALAANLASTLGLSPLGPYHSLMYGRSMYFDISKAQRELDFMPKY
CCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHEEEHHHHHHHHHCCCCC
SNSQMIIETYDWYQANKASLSKGRASRHKSPVKQQILALVPYVLRMAPG
CCCEEEEEECCCCCCCHHHHHHCHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVNTALVTGGSGYFGELLTKQLLEQGTHVRVFDLNFPGFSHPNLEFFKGTILDRNAVKQA
CCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHH
VSGVKKVFHNVAQVPLAKDVDLFWSINKDGTQILADESVAAGVVKLIYTSSSAVFGVPKA
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHEEECCCCEEECCCC
NPVTENTVTNPAEDYGRAKLAGEMICKEAMQRHGLDVAIVRPRTILGHGRLGIVQILFDW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHH
IERGLDIPVFAGGNNRYQFVHSDDLAWACIAASDMRGFAEYNIGAAEFGTMRELLQTVID
HHCCCCCEEEECCCCEEEEEECCCCEEEEEEHHHHCCHHHCCCCHHHHHHHHHHHHHHHH
HTGTESRIKSIPMRPTALAANLASTLGLSPLGPYHSLMYGRSMYFDISKAQRELDFMPKY
CCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCHHEEEHHHHHHHHHCCCCC
SNSQMIIETYDWYQANKASLSKGRASRHKSPVKQQILALVPYVLRMAPG
CCCEEEEEECCCCCCCHHHHHHCHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]