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The map label for this gene is yoaE [H]

Identifier: 13476571

GI number: 13476571

Start: 6548883

End: 6550394

Strand: Direct

Name: yoaE [H]

Synonym: mlr7933

Alternate gene names: 13476571

Gene position: 6548883-6550394 (Clockwise)

Preceding gene: 13476570

Following gene: 13476574

Centisome position: 93.08

GC content: 64.35

Gene sequence:

>1512_bases
ATGAACCAGCACGCCAAGCTTCGCATCGGCCATTCAGCCTGTCCGCATGATTGCCCGTCGACCTGCGCCCTCGAGGTCGA
ACTGCTCGACGGCAACCGCATCGGCCGCGTCCATGGCGCCAAGGCCAACACCTACACATCGGGCGTCGTCTGCGCCAAGG
TCGCCCGCTATGCCGACCGTGTCCATCATCCCGACCGCTTGCTGAAGCCGCTGATCCGCGCCGGCGCCAAGGGCGATGGC
GTCTGGCAGGAAGCGAGTTGGGAGGCGGCGCTCGACCTCGTCGCCGAAAAGTTCATCGCAGCCGAAGAGAAATACGGGTC
GGAGACGGTTTGGCCCTATTTCTATGCCGCCTCCTGCATCGCTGCGGTAACCGGCAGTTGGCAGTATGAGGGTGGCGGCG
CCTTCCATTCGAATTCCGGCATCTTCAAGCTCAACCAGGAGGTGCTGGAAGGCACCAGGATGCGCGATCCCTCGATCCGC
TATCTCGACCATTCGCGCATCGGCCCGGTGCTGACCGGCGCCAGCGACGCGCTCTATGGCGGGCCGCCGGTCACGGCGAT
GCTGATCCAGAACACCAATCCGGTGAATGTCGCTCCCGAGCAGCGGCTGGTGAAGCAGGGCTTTCTGCGCGACGACCTGT
TCGCTTGCGTGCACGAGCAGTTCATGACCGACACGGCCAGGCTTGCCGATGTGGTGCTGCCGGCGACGATGTTTCTGGAA
CATGACGACGTCTACAAGGGCGGCGGCAACCAGCACATCACGCTGGGGCCGAAGCTGATCGAGCCGCCGGAAGGGCCGCG
CACCAACCATTTCGTCATCGAGCAGCTGGCTGAGCGCCTGGGGGTCGCTGACCGCCCGGGTTTCGGCCTCACCGAACAGC
AGCATATCGACATCATTCTCGGCAAGCGCGGGCTGGGCAGCTTCGACAGCTTGAAAGAGCAGAAATGGGTGGATCTGCAG
CCGGATTTCGAGACCGCGCATTTCATCAAGGGGTTTGGCCATTCGGATGGGAAATTCCGCTTCCGCGCCGACTGGACGGG
CCAGGCGGCTCCCAACCGGCCGCCGAAGAGCATGGGCCTGTTCGGACCGGTGGAGCGCCTGCCTGAGTTTCCCGACCATG
TCGACCTGATCGAAGTGGCGGACGATGCGCATCCATTCAGGTTGACGACCTCGCCGGCGCGCAACTTCCTCAACTCGACC
TTTGCCGAGACGCCGGTGTCGAAGGAAAAGGAAGGCAGGCCGGCGTTGCTCCTGCATCCCGACGATGCAGCGGACCTTGG
GCTTGCCGATGGTGACCGCGTCGAGGTCGGCAACCAGCGCGGCGACCTGGTGCTGCATGCGAAATTCTTCGACGGCATCA
AGCGCGGCGTGGTGATCGCCGAAGGCATCTGGCCGAACAGCGCGCATGAGCGCGGCGAGGGCATCAACGTGCTGACCGGC
GCCGATGCGCCCGCGCCCTATGGCGGCGCTGCCTTCCACGACAACAAGGTCTGGCTGCGCAAGGCTGGTTGA

Upstream 100 bases:

>100_bases
GGCGGAGTAGGAGAGCGTTCTTTCCTTCTTTCCCTTGGGGGAGAAGGGAAGGCGCTATCTGGCCCTACACGCCACGCCCG
AAACTCGCTAGACATGCTTC

Downstream 100 bases:

>100_bases
TCAGAGCGAAGCGCTGGAGGCCGACTATACCGGCAGAATCTCAGCCGCCTTCTTCCTGTTCAGCCGGTGCCGGATGTCGT
CGGCGATCGCCTGGGCCTCG

Product: formate dehydrogenase

Products: Biotin [C]

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MNQHAKLRIGHSACPHDCPSTCALEVELLDGNRIGRVHGAKANTYTSGVVCAKVARYADRVHHPDRLLKPLIRAGAKGDG
VWQEASWEAALDLVAEKFIAAEEKYGSETVWPYFYAASCIAAVTGSWQYEGGGAFHSNSGIFKLNQEVLEGTRMRDPSIR
YLDHSRIGPVLTGASDALYGGPPVTAMLIQNTNPVNVAPEQRLVKQGFLRDDLFACVHEQFMTDTARLADVVLPATMFLE
HDDVYKGGGNQHITLGPKLIEPPEGPRTNHFVIEQLAERLGVADRPGFGLTEQQHIDIILGKRGLGSFDSLKEQKWVDLQ
PDFETAHFIKGFGHSDGKFRFRADWTGQAAPNRPPKSMGLFGPVERLPEFPDHVDLIEVADDAHPFRLTTSPARNFLNST
FAETPVSKEKEGRPALLLHPDDAADLGLADGDRVEVGNQRGDLVLHAKFFDGIKRGVVIAEGIWPNSAHERGEGINVLTG
ADAPAPYGGAAFHDNKVWLRKAG

Sequences:

>Translated_503_residues
MNQHAKLRIGHSACPHDCPSTCALEVELLDGNRIGRVHGAKANTYTSGVVCAKVARYADRVHHPDRLLKPLIRAGAKGDG
VWQEASWEAALDLVAEKFIAAEEKYGSETVWPYFYAASCIAAVTGSWQYEGGGAFHSNSGIFKLNQEVLEGTRMRDPSIR
YLDHSRIGPVLTGASDALYGGPPVTAMLIQNTNPVNVAPEQRLVKQGFLRDDLFACVHEQFMTDTARLADVVLPATMFLE
HDDVYKGGGNQHITLGPKLIEPPEGPRTNHFVIEQLAERLGVADRPGFGLTEQQHIDIILGKRGLGSFDSLKEQKWVDLQ
PDFETAHFIKGFGHSDGKFRFRADWTGQAAPNRPPKSMGLFGPVERLPEFPDHVDLIEVADDAHPFRLTTSPARNFLNST
FAETPVSKEKEGRPALLLHPDDAADLGLADGDRVEVGNQRGDLVLHAKFFDGIKRGVVIAEGIWPNSAHERGEGINVLTG
ADAPAPYGGAAFHDNKVWLRKAG
>Mature_503_residues
MNQHAKLRIGHSACPHDCPSTCALEVELLDGNRIGRVHGAKANTYTSGVVCAKVARYADRVHHPDRLLKPLIRAGAKGDG
VWQEASWEAALDLVAEKFIAAEEKYGSETVWPYFYAASCIAAVTGSWQYEGGGAFHSNSGIFKLNQEVLEGTRMRDPSIR
YLDHSRIGPVLTGASDALYGGPPVTAMLIQNTNPVNVAPEQRLVKQGFLRDDLFACVHEQFMTDTARLADVVLPATMFLE
HDDVYKGGGNQHITLGPKLIEPPEGPRTNHFVIEQLAERLGVADRPGFGLTEQQHIDIILGKRGLGSFDSLKEQKWVDLQ
PDFETAHFIKGFGHSDGKFRFRADWTGQAAPNRPPKSMGLFGPVERLPEFPDHVDLIEVADDAHPFRLTTSPARNFLNST
FAETPVSKEKEGRPALLLHPDDAADLGLADGDRVEVGNQRGDLVLHAKFFDGIKRGVVIAEGIWPNSAHERGEGINVLTG
ADAPAPYGGAAFHDNKVWLRKAG

Specific function: Reduces Trimethylamine-N-Oxide (Tmao) Into Trimethylamine; An Anaerobic Reaction Coupled To Energy-Yielding Reactions. Can Also Reduce Other N-And S-Oxide Compounds Such As 4-Methylmorpholine-N-Oxide And Biotin Sulfoxide (Bso), But With A Lower Catalytic

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=113, Percent_Identity=31.858407079646, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.7.2.3 [C]

Molecular weight: Translated: 55018; Mature: 55018

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQHAKLRIGHSACPHDCPSTCALEVELLDGNRIGRVHGAKANTYTSGVVCAKVARYADR
CCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VHHPDRLLKPLIRAGAKGDGVWQEASWEAALDLVAEKFIAAEEKYGSETVWPYFYAASCI
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AAVTGSWQYEGGGAFHSNSGIFKLNQEVLEGTRMRDPSIRYLDHSRIGPVLTGASDALYG
HHHHCCEEECCCCEEECCCCEEEECHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCCC
GPPVTAMLIQNTNPVNVAPEQRLVKQGFLRDDLFACVHEQFMTDTARLADVVLPATMFLE
CCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
HDDVYKGGGNQHITLGPKLIEPPEGPRTNHFVIEQLAERLGVADRPGFGLTEQQHIDIIL
CCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
GKRGLGSFDSLKEQKWVDLQPDFETAHFIKGFGHSDGKFRFRADWTGQAAPNRPPKSMGL
ECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHCCC
FGPVERLPEFPDHVDLIEVADDAHPFRLTTSPARNFLNSTFAETPVSKEKEGRPALLLHP
CCCHHHCCCCCCCCCCEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
DDAADLGLADGDRVEVGNQRGDLVLHAKFFDGIKRGVVIAEGIWPNSAHERGEGINVLTG
CCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHCCEEEEECCCCCCCHHCCCCCEEEEC
ADAPAPYGGAAFHDNKVWLRKAG
CCCCCCCCCCEEECCEEEEEECC
>Mature Secondary Structure
MNQHAKLRIGHSACPHDCPSTCALEVELLDGNRIGRVHGAKANTYTSGVVCAKVARYADR
CCCCCEEEECCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VHHPDRLLKPLIRAGAKGDGVWQEASWEAALDLVAEKFIAAEEKYGSETVWPYFYAASCI
CCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AAVTGSWQYEGGGAFHSNSGIFKLNQEVLEGTRMRDPSIRYLDHSRIGPVLTGASDALYG
HHHHCCEEECCCCEEECCCCEEEECHHHHCCCCCCCCCCEEECCCCCCCEEECCCCCCCC
GPPVTAMLIQNTNPVNVAPEQRLVKQGFLRDDLFACVHEQFMTDTARLADVVLPATMFLE
CCCEEEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
HDDVYKGGGNQHITLGPKLIEPPEGPRTNHFVIEQLAERLGVADRPGFGLTEQQHIDIIL
CCCCCCCCCCCEEEECCEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
GKRGLGSFDSLKEQKWVDLQPDFETAHFIKGFGHSDGKFRFRADWTGQAAPNRPPKSMGL
ECCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHCCC
FGPVERLPEFPDHVDLIEVADDAHPFRLTTSPARNFLNSTFAETPVSKEKEGRPALLLHP
CCCHHHCCCCCCCCCCEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
DDAADLGLADGDRVEVGNQRGDLVLHAKFFDGIKRGVVIAEGIWPNSAHERGEGINVLTG
CCCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHCCEEEEECCCCCCCHHCCCCCEEEEC
ADAPAPYGGAAFHDNKVWLRKAG
CCCCCCCCCCEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds) [C]

Specific reaction: Reduces A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), Back To Biotin. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]