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The map label for this gene is smpB

Identifier: 13476429

GI number: 13476429

Start: 6418041

End: 6418520

Strand: Direct

Name: smpB

Synonym: mlr7747

Alternate gene names: 13476429

Gene position: 6418041-6418520 (Clockwise)

Preceding gene: 13476428

Following gene: 13476430

Centisome position: 91.22

GC content: 59.58

Gene sequence:

>480_bases
ATGAATCAAGTCAGAAAAGCCGATCCCAACAACAAGACCGTTGCGGAAAATCGCAAGGCGCGGTTTTCCTATGAGGTGCT
CGACACGATCGAGGCCGGCTTGGTGCTGACCGGCACCGAGGTCAAGTCGCTGCGCCAGGGCCAGGCCAATATCCAGGACA
GCTATGCCTCGGTCGAAGGCGGCGAAATTTGGCTGATCAATTCCTATCTGCCCGAATATCTGCAGGCCAACCGCTTCAAC
CATGAGCCCAGGCGGCGGCGCAAGCTCCTGCTCAACAAGCGCGAGATGGCCAAGCTGTCGCAGAGCGTCGACCGTGAAGG
CATGACGCTGGTGCCGCTAAAAATCTATTTCAACGACCAGGGCCGCGCCAAGCTGCTGCTCGCCGTCGGCCGCGGCAAGA
AATTGCACGACAAGCGCGAAACCGAAAAGCAGCGCGACTGGTCGCGTGAGAAGGGCCGGCTCTTGAAGGAGCGTGGGTGA

Upstream 100 bases:

>100_bases
GATTTCTAGAGCGTCCTTTGCGCGTTTGATAACGCGCGGCGCTCTAGTGGCACCATCATCTGACTCGCAGGGGCGAGTTT
CGAAACGGACTCTAGACGTT

Downstream 100 bases:

>100_bases
GAGACGTCAGGCGGTAGCCCTCGAAAAGCCAGGTTGGCTTCCAAGTTTCGGGCAAGCGCAACCGGCAGATACCAGAGGGG
ATAGCCTTTGAAGAACCGGA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNQVRKADPNNKTVAENRKARFSYEVLDTIEAGLVLTGTEVKSLRQGQANIQDSYASVEGGEIWLINSYLPEYLQANRFN
HEPRRRRKLLLNKREMAKLSQSVDREGMTLVPLKIYFNDQGRAKLLLAVGRGKKLHDKRETEKQRDWSREKGRLLKERG

Sequences:

>Translated_159_residues
MNQVRKADPNNKTVAENRKARFSYEVLDTIEAGLVLTGTEVKSLRQGQANIQDSYASVEGGEIWLINSYLPEYLQANRFN
HEPRRRRKLLLNKREMAKLSQSVDREGMTLVPLKIYFNDQGRAKLLLAVGRGKKLHDKRETEKQRDWSREKGRLLKERG
>Mature_159_residues
MNQVRKADPNNKTVAENRKARFSYEVLDTIEAGLVLTGTEVKSLRQGQANIQDSYASVEGGEIWLINSYLPEYLQANRFN
HEPRRRRKLLLNKREMAKLSQSVDREGMTLVPLKIYFNDQGRAKLLLAVGRGKKLHDKRETEKQRDWSREKGRLLKERG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=41.4012738853503, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_RHILO (Q985B9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107999.1
- ProteinModelPortal:   Q985B9
- SMR:   Q985B9
- GeneID:   1230654
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7747
- NMPDR:   fig|266835.1.peg.6100
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18467; Mature: 18467

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVRKADPNNKTVAENRKARFSYEVLDTIEAGLVLTGTEVKSLRQGQANIQDSYASVEG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCHHHHHHCCCC
GEIWLINSYLPEYLQANRFNHEPRRRRKLLLNKREMAKLSQSVDREGMTLVPLKIYFNDQ
CEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCC
GRAKLLLAVGRGKKLHDKRETEKQRDWSREKGRLLKERG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MNQVRKADPNNKTVAENRKARFSYEVLDTIEAGLVLTGTEVKSLRQGQANIQDSYASVEG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECHHHHHHHHCCCCHHHHHHCCCC
GEIWLINSYLPEYLQANRFNHEPRRRRKLLLNKREMAKLSQSVDREGMTLVPLKIYFNDQ
CEEEEEHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCC
GRAKLLLAVGRGKKLHDKRETEKQRDWSREKGRLLKERG
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968