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The map label for this gene is livJ [C]

Identifier: 13476408

GI number: 13476408

Start: 6392981

End: 6394180

Strand: Direct

Name: livJ [C]

Synonym: mlr7721

Alternate gene names: 13476408

Gene position: 6392981-6394180 (Clockwise)

Preceding gene: 13476407

Following gene: 13476420

Centisome position: 90.86

GC content: 62.17

Gene sequence:

>1200_bases
ATGAAGGATGAAATTGTCCCGCCTCTCTGGAAGAAGCGCGGCCGGGCGGCCGCGCGTGAGTGGAGGAGTTCTATTGCCAT
GAAAAAAATGAGTATTTTGAGCGCGGCCATTTTCGGCCTGATGATGAGTGCGCCGGTCGCATTTGCCGACGACATCACCG
TTTCGGTGGTCGGTCCGATGACCGGACAGCTCGCCACTATCGGTGACCAGTTCAAGCAGGGCGCACAGGCCGCGGCAGAC
GCCATCAACGCCGCGGGCGGCGTCAACGGCTCGATGATCAAGCTCGACATCGAGGACGACCAGTGCGATCCCAAGCAGGC
CGTGTCGGTCGCCAACCGCATCATCGCCAACGGCGTCAAGTTCATCGACGGCCATGCCTGCTCGGGCTCGAGCATTCCCG
CCTCGGCCGTCTATGCCGAAGCCGGCGCGCTGATGATGAGCCCGGCTTCCTCGAACCCGGTGCTGACCGATGCGGCCGCC
AAGGCCGGCTGGCCGACCATCATGCGCCTCTATACGCGTGACGATGCACAGGGCGCCTTCATCGGCCCGTGGATCGCCAA
GAAATATGCCGGCAAGAACGTCGTCATCCTGCACGACAAGAGCGCTTACGGGCAGGGCGTGGCTGATGCCGTCAGGGCAA
CCATGAACACCGGCGGCCTCAAGGAAGTCTATTATGACGCCATCAATCCCGGCGAGAAGGACTATTCGGCGCTCGTCACC
AAGCTGAAGGATCTCAAGGCCGACGTCGTCTATTTCGGCGGCTACCATCCTGAAGCCGGCCTGATTCTGCGTCAGTCGGC
CGAACAGAACCTGAAGTTCCAGCTGATCATGCCGGACTCCATCGCCTCGCCGGAATTCTGGCAGGTTGCCGGCCCGGCTG
GCGAAGGCACCATGTTCGTCTTCCCGTCGGACCCGCAGGCAAAGCCGGAAGCCAAGGCCGCCGTCGAGAAGATCAAGGCC
GGCGGCTTCGTGCCGGAAGGCTTCACGCTGTTCTCTTACGCCGTGGTCCAGGCTTTCGCCGAAGGCATCAAGCGTGCCGG
CAGCGACGATCCGGCCAAGGTTGCCGAAGCGTTGAAGAACGGCACGCCGATCAGCACCGTTGTCGGCGACGTCACCTTCG
ACGAAAAGGGCGACCTCAAAAACGCCAGCTACGACATCAACCAGTGGCATGACGGCAAATACGCGCCGATCGCGCAGTAG

Upstream 100 bases:

>100_bases
AAAAAGCCACGACCAGCCGGAACAAGGCTGGCGTGGCTTTTTGCGTAGATGACGGCTCGCAGGCCCAATCGAGTGGCGCC
AATGCGCCTAGACAGGCCCA

Downstream 100 bases:

>100_bases
TCGCTGACATCACCTAGTGGCATTGCAGCGCCGCGCGTTCTTCTGGACGCGCGGCGCTCTGATGCAGCGGCCTAATAGGT
CACGGTAACGACAACGGTAT

Product: ABC transporter, binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKDEIVPPLWKKRGRAAAREWRSSIAMKKMSILSAAIFGLMMSAPVAFADDITVSVVGPMTGQLATIGDQFKQGAQAAAD
AINAAGGVNGSMIKLDIEDDQCDPKQAVSVANRIIANGVKFIDGHACSGSSIPASAVYAEAGALMMSPASSNPVLTDAAA
KAGWPTIMRLYTRDDAQGAFIGPWIAKKYAGKNVVILHDKSAYGQGVADAVRATMNTGGLKEVYYDAINPGEKDYSALVT
KLKDLKADVVYFGGYHPEAGLILRQSAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFVFPSDPQAKPEAKAAVEKIKA
GGFVPEGFTLFSYAVVQAFAEGIKRAGSDDPAKVAEALKNGTPISTVVGDVTFDEKGDLKNASYDINQWHDGKYAPIAQ

Sequences:

>Translated_399_residues
MKDEIVPPLWKKRGRAAAREWRSSIAMKKMSILSAAIFGLMMSAPVAFADDITVSVVGPMTGQLATIGDQFKQGAQAAAD
AINAAGGVNGSMIKLDIEDDQCDPKQAVSVANRIIANGVKFIDGHACSGSSIPASAVYAEAGALMMSPASSNPVLTDAAA
KAGWPTIMRLYTRDDAQGAFIGPWIAKKYAGKNVVILHDKSAYGQGVADAVRATMNTGGLKEVYYDAINPGEKDYSALVT
KLKDLKADVVYFGGYHPEAGLILRQSAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFVFPSDPQAKPEAKAAVEKIKA
GGFVPEGFTLFSYAVVQAFAEGIKRAGSDDPAKVAEALKNGTPISTVVGDVTFDEKGDLKNASYDINQWHDGKYAPIAQ
>Mature_399_residues
MKDEIVPPLWKKRGRAAAREWRSSIAMKKMSILSAAIFGLMMSAPVAFADDITVSVVGPMTGQLATIGDQFKQGAQAAAD
AINAAGGVNGSMIKLDIEDDQCDPKQAVSVANRIIANGVKFIDGHACSGSSIPASAVYAEAGALMMSPASSNPVLTDAAA
KAGWPTIMRLYTRDDAQGAFIGPWIAKKYAGKNVVILHDKSAYGQGVADAVRATMNTGGLKEVYYDAINPGEKDYSALVT
KLKDLKADVVYFGGYHPEAGLILRQSAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFVFPSDPQAKPEAKAAVEKIKA
GGFVPEGFTLFSYAVVQAFAEGIKRAGSDDPAKVAEALKNGTPISTVVGDVTFDEKGDLKNASYDINQWHDGKYAPIAQ

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=346, Percent_Identity=41.3294797687861, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI1789867, Length=347, Percent_Identity=40.3458213256484, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42203; Mature: 42203

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEIVPPLWKKRGRAAAREWRSSIAMKKMSILSAAIFGLMMSAPVAFADDITVSVVGPM
CCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCC
TGQLATIGDQFKQGAQAAADAINAAGGVNGSMIKLDIEDDQCDPKQAVSVANRIIANGVK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC
FIDGHACSGSSIPASAVYAEAGALMMSPASSNPVLTDAAAKAGWPTIMRLYTRDDAQGAF
EECCCCCCCCCCCHHHHHHHCCEEEECCCCCCCEEECHHHHCCCHHHEEEEECCCCCCCE
IGPWIAKKYAGKNVVILHDKSAYGQGVADAVRATMNTGGLKEVYYDAINPGEKDYSALVT
ECHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
KLKDLKADVVYFGGYHPEAGLILRQSAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFV
HHHHCCCCEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEECCCCCCEEEE
FPSDPQAKPEAKAAVEKIKAGGFVPEGFTLFSYAVVQAFAEGIKRAGSDDPAKVAEALKN
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GTPISTVVGDVTFDEKGDLKNASYDINQWHDGKYAPIAQ
CCCHHHHEECCEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKDEIVPPLWKKRGRAAAREWRSSIAMKKMSILSAAIFGLMMSAPVAFADDITVSVVGPM
CCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEEECCC
TGQLATIGDQFKQGAQAAADAINAAGGVNGSMIKLDIEDDQCDPKQAVSVANRIIANGVK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC
FIDGHACSGSSIPASAVYAEAGALMMSPASSNPVLTDAAAKAGWPTIMRLYTRDDAQGAF
EECCCCCCCCCCCHHHHHHHCCEEEECCCCCCCEEECHHHHCCCHHHEEEEECCCCCCCE
IGPWIAKKYAGKNVVILHDKSAYGQGVADAVRATMNTGGLKEVYYDAINPGEKDYSALVT
ECHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHH
KLKDLKADVVYFGGYHPEAGLILRQSAEQNLKFQLIMPDSIASPEFWQVAGPAGEGTMFV
HHHHCCCCEEEECCCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEECCCCCCEEEE
FPSDPQAKPEAKAAVEKIKAGGFVPEGFTLFSYAVVQAFAEGIKRAGSDDPAKVAEALKN
ECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
GTPISTVVGDVTFDEKGDLKNASYDINQWHDGKYAPIAQ
CCCHHHHEECCEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA