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The map label for this gene is gcvA [H]

Identifier: 13476373

GI number: 13476373

Start: 6353185

End: 6354072

Strand: Direct

Name: gcvA [H]

Synonym: mlr7672

Alternate gene names: 13476373

Gene position: 6353185-6354072 (Clockwise)

Preceding gene: 13476358

Following gene: 13476374

Centisome position: 90.29

GC content: 65.77

Gene sequence:

>888_bases
TTGAGAATTCCATCCACACAGGCGCTGCGCGCCCTCGACAGTTTCGCCCGCCACGGCAGCGTCTGGCGTGCTGCCGACGA
ACTGCACCTCACCCGCAGCGCGGTGAGCCACCAGTTGCGGCTTTTGGAACGCGATCTCGGCTTCGACCTGCTGCAGCGCA
TCGGCAAGGGGGTGGCGCTGACGCCGCGCGGCCAGCGCTACGCAGCCGATGTCAGGAAGGCGCTGACCGTGCTCGGCGAT
GCGGGGGCGCAAAACAGCGGCGCCGGCGTCGGCGGTTCTTTCGCCATTTCCTGCCCGCCCGGCTTCGCCTCGATGTTCCT
GTGCAGCCATATTGGCGAATTCCAGCAGATGTACCCGGACGTGGCGCTCTCGGTGCTGACGCCGCGTCGTCTCGACGACG
TCAGCAATCCCGAGGCCGACGCCTTCATCGCCTTCGGCGTCGGCAACTGGCCGAACCGGGTGGTGGAACTGCTCTGCGAA
GTTCACTTCACGCCGCTCTGCAGCCCGACATTGCTCAACAAGGTCGGCGGCTTTTCCAAGCCGGCGGACGTGCTGCGCGC
CAACCTGCTGCATCTCGGCGATACCGAGGACTGGGCGCGCTGGCTGGCTCTGTCCAAGGTGGAAAACCCGGATACCGAAG
GCGGTATCTTCTTCTCCGACATGAACCTGGTCTTCTCGGCGGCGGTCGCCGGCCAGGGAATTGCCATGGGCGACGAGCTG
ACCGGCCGCCGGGCACTGAGCGAAGGCCGGCTGGTCAAGCCGTTTGAAGCGTCCGTGCCCTCGCCACGCTCCTATTTCCT
GGTCTTCGAACACGCCAAGGCCGGCCATCCCGTGCTCAACGCCTTCAGCGACTGGCTCAGGGCAAAGCTGTCGGAGAACA
GGCTCTAG

Upstream 100 bases:

>100_bases
CCGATGTCGTCACCGGTTTGTCCATGCATCAAAGAAAGCAGTGAAGCGGCTCGCCAAAAAGTGATATGTTTTCCCGGTTC
ATGCAAATTCGGTTCACCTC

Downstream 100 bases:

>100_bases
CCCTATTGAGCACCGCTCCTTTTCGCCAGACGTGAATCAAATTTGCCGATCAGGCGAAAACTATTCGGTTGCGGTGAGCC
GCCGGCCTGCCTACGATTTC

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRIPSTQALRALDSFARHGSVWRAADELHLTRSAVSHQLRLLERDLGFDLLQRIGKGVALTPRGQRYAADVRKALTVLGD
AGAQNSGAGVGGSFAISCPPGFASMFLCSHIGEFQQMYPDVALSVLTPRRLDDVSNPEADAFIAFGVGNWPNRVVELLCE
VHFTPLCSPTLLNKVGGFSKPADVLRANLLHLGDTEDWARWLALSKVENPDTEGGIFFSDMNLVFSAAVAGQGIAMGDEL
TGRRALSEGRLVKPFEASVPSPRSYFLVFEHAKAGHPVLNAFSDWLRAKLSENRL

Sequences:

>Translated_295_residues
MRIPSTQALRALDSFARHGSVWRAADELHLTRSAVSHQLRLLERDLGFDLLQRIGKGVALTPRGQRYAADVRKALTVLGD
AGAQNSGAGVGGSFAISCPPGFASMFLCSHIGEFQQMYPDVALSVLTPRRLDDVSNPEADAFIAFGVGNWPNRVVELLCE
VHFTPLCSPTLLNKVGGFSKPADVLRANLLHLGDTEDWARWLALSKVENPDTEGGIFFSDMNLVFSAAVAGQGIAMGDEL
TGRRALSEGRLVKPFEASVPSPRSYFLVFEHAKAGHPVLNAFSDWLRAKLSENRL
>Mature_295_residues
MRIPSTQALRALDSFARHGSVWRAADELHLTRSAVSHQLRLLERDLGFDLLQRIGKGVALTPRGQRYAADVRKALTVLGD
AGAQNSGAGVGGSFAISCPPGFASMFLCSHIGEFQQMYPDVALSVLTPRRLDDVSNPEADAFIAFGVGNWPNRVVELLCE
VHFTPLCSPTLLNKVGGFSKPADVLRANLLHLGDTEDWARWLALSKVENPDTEGGIFFSDMNLVFSAAVAGQGIAMGDEL
TGRRALSEGRLVKPFEASVPSPRSYFLVFEHAKAGHPVLNAFSDWLRAKLSENRL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.6388888888889, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=27.5862068965517, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=27.5261324041812, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=26.3698630136986, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI157672245, Length=153, Percent_Identity=31.3725490196078, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=23.0769230769231, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32055; Mature: 32055

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPSTQALRALDSFARHGSVWRAADELHLTRSAVSHQLRLLERDLGFDLLQRIGKGVAL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEE
TPRGQRYAADVRKALTVLGDAGAQNSGAGVGGSFAISCPPGFASMFLCSHIGEFQQMYPD
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
VALSVLTPRRLDDVSNPEADAFIAFGVGNWPNRVVELLCEVHFTPLCSPTLLNKVGGFSK
HHHHHHCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCC
PADVLRANLLHLGDTEDWARWLALSKVENPDTEGGIFFSDMNLVFSAAVAGQGIAMGDEL
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHH
TGRRALSEGRLVKPFEASVPSPRSYFLVFEHAKAGHPVLNAFSDWLRAKLSENRL
HHHHHHCCCCEECCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIPSTQALRALDSFARHGSVWRAADELHLTRSAVSHQLRLLERDLGFDLLQRIGKGVAL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEE
TPRGQRYAADVRKALTVLGDAGAQNSGAGVGGSFAISCPPGFASMFLCSHIGEFQQMYPD
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
VALSVLTPRRLDDVSNPEADAFIAFGVGNWPNRVVELLCEVHFTPLCSPTLLNKVGGFSK
HHHHHHCCHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCC
PADVLRANLLHLGDTEDWARWLALSKVENPDTEGGIFFSDMNLVFSAAVAGQGIAMGDEL
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHH
TGRRALSEGRLVKPFEASVPSPRSYFLVFEHAKAGHPVLNAFSDWLRAKLSENRL
HHHHHHCCCCEECCHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]