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The map label for this gene is hyi [C]

Identifier: 13476339

GI number: 13476339

Start: 6320816

End: 6321598

Strand: Direct

Name: hyi [C]

Synonym: mlr7629

Alternate gene names: 13476339

Gene position: 6320816-6321598 (Clockwise)

Preceding gene: 13476338

Following gene: 13476340

Centisome position: 89.83

GC content: 64.62

Gene sequence:

>783_bases
ATGTCCGATACCTTTACGCTTGCGGCTTGCGCCGAGATGCTGTGGCGCGACAAGCCGATGGAATGGCGCGTCAAGCGCCT
CACCGAGTTGGGCTTCCAGGTCGGCCTCTGGAACTGGCCGGACCATGACCTTGCGATGCTGGAAAGATCCGGCGCGACCT
TCTCGATCATGAACGGCTATCTCAGCGGCAGGCTTTCTGATGACGAGGGCGCTGCCGAACTGTTGAAGACGGCGAAGGAG
ACGGTGGCGGTAGGCAAGCGTCTCGGCGTCGCGCGGCTCAATCTGCACGGCACGGGGCTCGGCGAAGGCGGCCTGCCGGT
CACTCCTTGCGAGACCGTCACCGGCGCTATGTGGCTCAAGGCCCGCGACACGCTGAACCGCATCGCCGACCTCGCCGAGG
CGGAAGGCGTGACCTTCACGCTGGAGAATTTGAACCTGCCGGTCGACCATCCCGGCGTGCCGTTTGGGCGCGCGGAAGAC
ACGCTAGCGCTAGTGTCTGATGTCAACCGGCCTGGTCTGCGTCTCAACCTCGACCTCTACCATGCGCAGATTGGCGAAGG
GAATTTGATCGAACTGTGCCGCACCTGCTTGCCCTGGATCGGCGAGGTCCAGGTGGCCGACGTGCCCGGACGCATGGAGC
CCGGCAGCGGCGAGATCAATTACCGGGGTATCGCCCATGCGCTGAAGGACATGGGCTACCGCGGGCCGGTGGGCATGGAG
GCGTTCGCCTCCGGCGATCCTGAGGCCGCCCTGCAAGCCTTCCGCGAAGCTTTCACCATCTGA

Upstream 100 bases:

>100_bases
GATTACCGCACCGGCGAAGCACTACGCGCACCGGTCTGCGTGAACCTGAAAACCTACGCCACGAAGGTCGAGGACGGCCG
CGTGTACATCGAGGTCTGAC

Downstream 100 bases:

>100_bases
GGGAACAAAATGGTCAGCAAAAATACCCGGCCCACCGTCGCCGACATCCGCGCCATGAAGGGCCGCGGACAGAAGTTCTC
CATGCTCTACGTCACTTCGC

Product: hypothetical protein

Products: 2-Hydroxy-3-oxopropanoate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSDTFTLAACAEMLWRDKPMEWRVKRLTELGFQVGLWNWPDHDLAMLERSGATFSIMNGYLSGRLSDDEGAAELLKTAKE
TVAVGKRLGVARLNLHGTGLGEGGLPVTPCETVTGAMWLKARDTLNRIADLAEAEGVTFTLENLNLPVDHPGVPFGRAED
TLALVSDVNRPGLRLNLDLYHAQIGEGNLIELCRTCLPWIGEVQVADVPGRMEPGSGEINYRGIAHALKDMGYRGPVGME
AFASGDPEAALQAFREAFTI

Sequences:

>Translated_260_residues
MSDTFTLAACAEMLWRDKPMEWRVKRLTELGFQVGLWNWPDHDLAMLERSGATFSIMNGYLSGRLSDDEGAAELLKTAKE
TVAVGKRLGVARLNLHGTGLGEGGLPVTPCETVTGAMWLKARDTLNRIADLAEAEGVTFTLENLNLPVDHPGVPFGRAED
TLALVSDVNRPGLRLNLDLYHAQIGEGNLIELCRTCLPWIGEVQVADVPGRMEPGSGEINYRGIAHALKDMGYRGPVGME
AFASGDPEAALQAFREAFTI
>Mature_259_residues
SDTFTLAACAEMLWRDKPMEWRVKRLTELGFQVGLWNWPDHDLAMLERSGATFSIMNGYLSGRLSDDEGAAELLKTAKET
VAVGKRLGVARLNLHGTGLGEGGLPVTPCETVTGAMWLKARDTLNRIADLAEAEGVTFTLENLNLPVDHPGVPFGRAEDT
LALVSDVNRPGLRLNLDLYHAQIGEGNLIELCRTCLPWIGEVQVADVPGRMEPGSGEINYRGIAHALKDMGYRGPVGMEA
FASGDPEAALQAFREAFTI

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=169, Percent_Identity=33.7278106508876, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI300068964, Length=161, Percent_Identity=33.5403726708075, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1786718, Length=133, Percent_Identity=34.5864661654135, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789095, Length=132, Percent_Identity=34.0909090909091, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22 [C]

Molecular weight: Translated: 28265; Mature: 28133

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTFTLAACAEMLWRDKPMEWRVKRLTELGFQVGLWNWPDHDLAMLERSGATFSIMNGY
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCEEEEEHHH
LSGRLSDDEGAAELLKTAKETVAVGKRLGVARLNLHGTGLGEGGLPVTPCETVTGAMWLK
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ARDTLNRIADLAEAEGVTFTLENLNLPVDHPGVPFGRAEDTLALVSDVNRPGLRLNLDLY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEE
HAQIGEGNLIELCRTCLPWIGEVQVADVPGRMEPGSGEINYRGIAHALKDMGYRGPVGME
EEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
AFASGDPEAALQAFREAFTI
HHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTFTLAACAEMLWRDKPMEWRVKRLTELGFQVGLWNWPDHDLAMLERSGATFSIMNGY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHCCCCEEEEEHHH
LSGRLSDDEGAAELLKTAKETVAVGKRLGVARLNLHGTGLGEGGLPVTPCETVTGAMWLK
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
ARDTLNRIADLAEAEGVTFTLENLNLPVDHPGVPFGRAEDTLALVSDVNRPGLRLNLDLY
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEEE
HAQIGEGNLIELCRTCLPWIGEVQVADVPGRMEPGSGEINYRGIAHALKDMGYRGPVGME
EEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCH
AFASGDPEAALQAFREAFTI
HHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: Hydroxypyruvate [C]

Specific reaction: Hydroxypyruvate <==> 2-Hydroxy-3-oxopropanoate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]