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The map label for this gene is cysD

Identifier: 13476292

GI number: 13476292

Start: 6259568

End: 6260473

Strand: Direct

Name: cysD

Synonym: mlr7575

Alternate gene names: 13476292

Gene position: 6259568-6260473 (Clockwise)

Preceding gene: 13476289

Following gene: 13476293

Centisome position: 88.96

GC content: 59.71

Gene sequence:

>906_bases
ATGACAATCGCGCTTACGCATCTGCAGCGGCTTGAAGCCGAGTCCATCCACATTTTCCGCGAGGTTGCGGCCGCCTTTGC
CAAGCCGGTCATGCTCTATTCGGTCGGCAAGGATTCATCCGTGCTGATGCATCTGGCAATGAAGGCGTTCTATCCCGCCA
AGCCGCCGTTCCCGTTTCTTCATGTCGACACCACCTGGAAGTTCCGCGAGATGATCGCTTTCCGCGATCAGATGGCGCAA
AAACTCGGCTTCGACCTTTTGGTGCATGTCAATGAAGACGGCGTGCGCGACAACATCAATCCCTTCGATCACGGCTCGAA
CACCCATACCCATGTGATGAAGACGGTGGCGCTGCGCCAGGCGCTGGACAAATACGGTTTCGATGCCGCCTTTGGCGGCG
CCCGGCGCGACGAGGAAAAGTCGCGCGCCAAGGAGCGCATATTCTCGTTCCGCAATGCCCAGCATGTCTGGGATCCGAAG
AACCAGCGCCCCGAAATGTGGAAGATCTTCAACACCCGCATCGCGTCGGGCGAATCGATTCGCGTCTTCCCGCTGTCGAA
CTGGACCGAGCTCGATATCTGGCAGTACATCCTGCAGGAAAACATCCCGATCGTGCCGCTCTATTTCGCCAAGGAACGGC
CGGTGGTGGAGCGCGACGGGATGTTGATCCTCAAGGACGACGACCGCATGAAACTGCGCCCCGGCGAGACAGTGGAGAAC
CGCCTGGTGCGGTTCCGCACTTTGGGCTGCTATCCGCTTACCGGCGCCATCGAATCCGATGCCGACACGCTCGAAGCCAT
CGTCGGCGAGATGCTGACCGCGCGCACATCCGAGCGGCAGGGCCGGCTGATCGACCGTGACGAAGCCGGCTCGATGGAAA
AGAAGAAGCGCGAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
GAAACACTTTGTCCAGAAGCGAAGCTTGAACCGGGCACAAAAGCTCGATGATTTGACGCAAGGTCTTCTGTCGCAAGGCA
CGAGAGAACGCTCACAGTCT

Downstream 100 bases:

>100_bases
CCATGCGTCACATCATGGCAAAAAGCCTCGCCCCCACCGACAGCGTCCGCGACTACCTGGCCGCGCAGGAGAAGAAGTCG
CTGCTGCGCTTCCTGACCTG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTIALTHLQRLEAESIHIFREVAAAFAKPVMLYSVGKDSSVLMHLAMKAFYPAKPPFPFLHVDTTWKFREMIAFRDQMAQ
KLGFDLLVHVNEDGVRDNINPFDHGSNTHTHVMKTVALRQALDKYGFDAAFGGARRDEEKSRAKERIFSFRNAQHVWDPK
NQRPEMWKIFNTRIASGESIRVFPLSNWTELDIWQYILQENIPIVPLYFAKERPVVERDGMLILKDDDRMKLRPGETVEN
RLVRFRTLGCYPLTGAIESDADTLEAIVGEMLTARTSERQGRLIDRDEAGSMEKKKREGYF

Sequences:

>Translated_301_residues
MTIALTHLQRLEAESIHIFREVAAAFAKPVMLYSVGKDSSVLMHLAMKAFYPAKPPFPFLHVDTTWKFREMIAFRDQMAQ
KLGFDLLVHVNEDGVRDNINPFDHGSNTHTHVMKTVALRQALDKYGFDAAFGGARRDEEKSRAKERIFSFRNAQHVWDPK
NQRPEMWKIFNTRIASGESIRVFPLSNWTELDIWQYILQENIPIVPLYFAKERPVVERDGMLILKDDDRMKLRPGETVEN
RLVRFRTLGCYPLTGAIESDADTLEAIVGEMLTARTSERQGRLIDRDEAGSMEKKKREGYF
>Mature_300_residues
TIALTHLQRLEAESIHIFREVAAAFAKPVMLYSVGKDSSVLMHLAMKAFYPAKPPFPFLHVDTTWKFREMIAFRDQMAQK
LGFDLLVHVNEDGVRDNINPFDHGSNTHTHVMKTVALRQALDKYGFDAAFGGARRDEEKSRAKERIFSFRNAQHVWDPKN
QRPEMWKIFNTRIASGESIRVFPLSNWTELDIWQYILQENIPIVPLYFAKERPVVERDGMLILKDDDRMKLRPGETVENR
LVRFRTLGCYPLTGAIESDADTLEAIVGEMLTARTSERQGRLIDRDEAGSMEKKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=299, Percent_Identity=70.9030100334448, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_RHILO (Q985Q5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107862.1
- HSSP:   Q87WW0
- ProteinModelPortal:   Q985Q5
- SMR:   Q985Q5
- GeneID:   1230517
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7575
- NMPDR:   fig|266835.1.peg.5963
- HOGENOM:   HBG758022
- OMA:   HYGFDAA
- ProtClustDB:   PRK05253
- BRENDA:   2.7.7.4
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 34821; Mature: 34690

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALTHLQRLEAESIHIFREVAAAFAKPVMLYSVGKDSSVLMHLAMKAFYPAKPPFPFL
CEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEE
HVDTTWKFREMIAFRDQMAQKLGFDLLVHVNEDGVRDNINPFDHGSNTHTHVMKTVALRQ
EECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALDKYGFDAAFGGARRDEEKSRAKERIFSFRNAQHVWDPKNQRPEMWKIFNTRIASGESI
HHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
RVFPLSNWTELDIWQYILQENIPIVPLYFAKERPVVERDGMLILKDDDRMKLRPGETVEN
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCHHHH
RLVRFRTLGCYPLTGAIESDADTLEAIVGEMLTARTSERQGRLIDRDEAGSMEKKKREGY
HHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHCCC
F
C
>Mature Secondary Structure 
TIALTHLQRLEAESIHIFREVAAAFAKPVMLYSVGKDSSVLMHLAMKAFYPAKPPFPFL
EEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCEE
HVDTTWKFREMIAFRDQMAQKLGFDLLVHVNEDGVRDNINPFDHGSNTHTHVMKTVALRQ
EECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALDKYGFDAAFGGARRDEEKSRAKERIFSFRNAQHVWDPKNQRPEMWKIFNTRIASGESI
HHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEE
RVFPLSNWTELDIWQYILQENIPIVPLYFAKERPVVERDGMLILKDDDRMKLRPGETVEN
EEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCHHHH
RLVRFRTLGCYPLTGAIESDADTLEAIVGEMLTARTSERQGRLIDRDEAGSMEKKKREGY
HHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968