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The map label for this gene is rfbB [C]

Identifier: 13476270

GI number: 13476270

Start: 6230898

End: 6231926

Strand: Direct

Name: rfbB [C]

Synonym: mlr7549

Alternate gene names: 13476270

Gene position: 6230898-6231926 (Clockwise)

Preceding gene: 13476269

Following gene: 13476271

Centisome position: 88.56

GC content: 63.85

Gene sequence:

>1029_bases
ATGAAGGTTCTGGTCACCGGCGCCGCCGGCTTCATAGGCTATCATGTCGCCAGGCGGCTCCTGGAGCGTGGCGACGAGGT
TGTCGGCATCGACAGCGTCAACGACTATTATGACCCGCGGATCAAGCAGGCGCGGCTGCGGCTGCTGGCCGAAGCCAGCC
GCGGCAGCAATGCCGGCTATCATTTCATCCACGGCAATCTTGCCGAGAGAGAAATCGTGGACGGCTGCTTTGCCGATCAC
GACTTCGACCGGGTGATCCATCTCGCCGCCCAGGCCGGGGTCCGCTACAGCCTGGAAAACCCGCGCGCCTATGTCGAAAG
CAACATCGTCGCTTTCACCAACATGCTGGAGGCCTGCCGCAATGCCGGCATGGCGCACCTGACCTATGCCAGCACCTCGA
GTGTTTACGGCGCCAACACCGACATGCCGTTTTCGGAGCACCGCCCGGCCGATCATCCGCTGCAGTTCTACGCCGCGACC
AAGCGCGCCAACGAGCTGATGGCGCACAGCTACAGCCATCTGTTCGGGCTGCCGACCACCGGGCTGCGGTTCTTCACCGT
ATACGGCCCCTGGGGGCGTCCCGACATGGCGCTGTTCCTGTTTACAAGGAGCATCCTGGCCGGCGAGCCGATCAAGCTGT
TCAACAATGGCAACCACACGCGCGACTTCACCTATATCGATGACATCGCCGAAGGTGTGATCCGGGCCAGCGACAGTCCT
GCCGCCGGCAATCCCGCCTGGGATTCAGGCCATCCGGATCCGGCAACGAGCAGCGCGCCCTGGCGCATCTTCAACATCGG
CAACAACAATCCGGTCAAGCTGACCGCCTATGTCGAGGCGCTGGAAAGCGCGCTTGGCCGCAAGGCCGTCATCGAGCTCT
TGCCGCTGCAGGCCGGCGACGTGCCGGACACTTTCGCCGACACCACCGCGCTGCAGGAGGCTGTTGGCTACCGCCCCGGC
ACGTCCGTGTCGGACGGGGTAGGGCGGTTCGTCGAGTGGTACAAGGCGTATTTTGGGTGGGGTGGCTGA

Upstream 100 bases:

>100_bases
GTGCCGGCCGTCTGGTCGCTGCTGAAGCGCTCCTGAGGTCTGTAGGAAGCGTGGCGTTCAATAGGCGTCAGGGGTCCATT
CCTGAGGAAGGGCGCTTCCG

Downstream 100 bases:

>100_bases
GGTCATGTCCCGGCGTGCGAAACAGACCCCTTCTGTGTCGCGTGCCGCATTGACAAGCCATTGAATGAAACCTAAGCACG
GGCGGTCAGGGCCGGGGGGC

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKVLVTGAAGFIGYHVARRLLERGDEVVGIDSVNDYYDPRIKQARLRLLAEASRGSNAGYHFIHGNLAEREIVDGCFADH
DFDRVIHLAAQAGVRYSLENPRAYVESNIVAFTNMLEACRNAGMAHLTYASTSSVYGANTDMPFSEHRPADHPLQFYAAT
KRANELMAHSYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTRSILAGEPIKLFNNGNHTRDFTYIDDIAEGVIRASDSP
AAGNPAWDSGHPDPATSSAPWRIFNIGNNNPVKLTAYVEALESALGRKAVIELLPLQAGDVPDTFADTTALQEAVGYRPG
TSVSDGVGRFVEWYKAYFGWGG

Sequences:

>Translated_342_residues
MKVLVTGAAGFIGYHVARRLLERGDEVVGIDSVNDYYDPRIKQARLRLLAEASRGSNAGYHFIHGNLAEREIVDGCFADH
DFDRVIHLAAQAGVRYSLENPRAYVESNIVAFTNMLEACRNAGMAHLTYASTSSVYGANTDMPFSEHRPADHPLQFYAAT
KRANELMAHSYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTRSILAGEPIKLFNNGNHTRDFTYIDDIAEGVIRASDSP
AAGNPAWDSGHPDPATSSAPWRIFNIGNNNPVKLTAYVEALESALGRKAVIELLPLQAGDVPDTFADTTALQEAVGYRPG
TSVSDGVGRFVEWYKAYFGWGG
>Mature_342_residues
MKVLVTGAAGFIGYHVARRLLERGDEVVGIDSVNDYYDPRIKQARLRLLAEASRGSNAGYHFIHGNLAEREIVDGCFADH
DFDRVIHLAAQAGVRYSLENPRAYVESNIVAFTNMLEACRNAGMAHLTYASTSSVYGANTDMPFSEHRPADHPLQFYAAT
KRANELMAHSYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTRSILAGEPIKLFNNGNHTRDFTYIDDIAEGVIRASDSP
AAGNPAWDSGHPDPATSSAPWRIFNIGNNNPVKLTAYVEALESALGRKAVIELLPLQAGDVPDTFADTTALQEAVGYRPG
TSVSDGVGRFVEWYKAYFGWGG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=26.3768115942029, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI42516563, Length=347, Percent_Identity=23.6311239193084, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56237023, Length=352, Percent_Identity=24.1477272727273, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI56118217, Length=352, Percent_Identity=24.1477272727273, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI189083684, Length=352, Percent_Identity=24.1477272727273, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=27.2980501392758, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994969, Length=363, Percent_Identity=25.6198347107438, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=23.1454005934718, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=347, Percent_Identity=25.9365994236311, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=23.5127478753541, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=24.2937853107345, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=345, Percent_Identity=24.9275362318841, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=238, Percent_Identity=25.6302521008403, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=361, Percent_Identity=25.207756232687, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=334, Percent_Identity=24.5508982035928, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=24.2690058479532, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37527; Mature: 37527

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGAAGFIGYHVARRLLERGDEVVGIDSVNDYYDPRIKQARLRLLAEASRGSNAGY
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCCCCCCE
HFIHGNLAEREIVDGCFADHDFDRVIHLAAQAGVRYSLENPRAYVESNIVAFTNMLEACR
EEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCHHHHHCCHHHHHHHHHHHH
NAGMAHLTYASTSSVYGANTDMPFSEHRPADHPLQFYAATKRANELMAHSYSHLFGLPTT
CCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLRFFTVYGPWGRPDMALFLFTRSILAGEPIKLFNNGNHTRDFTYIDDIAEGVIRASDSP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCC
AAGNPAWDSGHPDPATSSAPWRIFNIGNNNPVKLTAYVEALESALGRKAVIELLPLQAGD
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHCHHHHHHHHCCCCCC
VPDTFADTTALQEAVGYRPGTSVSDGVGRFVEWYKAYFGWGG
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVLVTGAAGFIGYHVARRLLERGDEVVGIDSVNDYYDPRIKQARLRLLAEASRGSNAGY
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCHHCCHHHHHHHHHHHHHHCCCCCCCE
HFIHGNLAEREIVDGCFADHDFDRVIHLAAQAGVRYSLENPRAYVESNIVAFTNMLEACR
EEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCHHHHHCCHHHHHHHHHHHH
NAGMAHLTYASTSSVYGANTDMPFSEHRPADHPLQFYAATKRANELMAHSYSHLFGLPTT
CCCCEEEEECCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLRFFTVYGPWGRPDMALFLFTRSILAGEPIKLFNNGNHTRDFTYIDDIAEGVIRASDSP
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCC
AAGNPAWDSGHPDPATSSAPWRIFNIGNNNPVKLTAYVEALESALGRKAVIELLPLQAGD
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHHCHHHHHHHHCCCCCC
VPDTFADTTALQEAVGYRPGTSVSDGVGRFVEWYKAYFGWGG
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]