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The map label for this gene is ppdK [H]

Identifier: 13476256

GI number: 13476256

Start: 6215703

End: 6218381

Strand: Direct

Name: ppdK [H]

Synonym: mlr7532

Alternate gene names: 13476256

Gene position: 6215703-6218381 (Clockwise)

Preceding gene: 13476253

Following gene: 13476257

Centisome position: 88.34

GC content: 64.84

Gene sequence:

>2679_bases
ATGACCAAATGGGTTTTCACCTTTGGCGATGGTGCGGCGGAAGGCCGTGCCGGCGACAAGAATCTGCTGGGCGGCAAGGG
CGCCAACCTGGCCGAAATGTGCAGCCTGGGCCTGCCGGTGCCGCCAGGCTTCACCATCACCACCGAGGTCTGCAATGCCT
ACTATGCCAATGGCCGCGCCTATCCGGCGGGATTGGAAGCCGACGTCGCGGTCGCGCTCGATCACATCGGCCGGCTGACT
GACCGCCGGTTCGGCGATCCGTCGAAGCTGCTGCTGGTGTCGGTGCGCTCCGGAGCTCGCGCCTCGATGCCGGGCATGAT
GGACACCGTGCTCAATCTTGGCCTCAACGACGAGACGGTCGAGGCGCTGGCCACCGATTCCGGTGATGCGCGCTTTGCCT
ATGACAGCTACCGCCGCTTCATCCAGATGTATTCCGATGTCGTCATGGGCCTCGATCACGAGGTGTTCGAGGAAATCCTC
GAAGACCAGAAGGCCAGCCTCGGCCATGAGCTCGACACCGAACTGACGGCCATCGAATGGCAGGGCGTGATCGCGCTCTA
CAAGGCCAAGGTCGAGGAAGAGCTCGGCAGGCCGTTCCCGCAGGATCCGCAGGAGCAGCTCTGGGGCGCTATCGGCGCGG
TGTTCTCGAGCTGGATGAACAACCGCGCCATCACCTACCGGCGCCTGCACGACATCCCCGAAAGCTGGGGCACGGCGGTC
AACGTCCAGGCCATGGTGTTCGGCAATATGGGCGAGACCTCGGCCACCGGCGTTGCCTTCACCCGCAACCCATCGACCGG
CGAAAAGCAGCTCTATGGCGAGTTCCTGGTGAACGCACAGGGCGAGGATGTCGTCGCCGGCATCCGCACGCCGCAGAACA
TCACCGAGGCCGCCCGCATCGCCGCCGGCTCCGACAAGCCGTCGCTGCAGAAGCTGATGCCCGATGCCTTCCAATCCTTC
GTCAGCATCTCCGACAGCCTGGAAAAGCACTATCGCGACATGCAGGACCTCGAATTCACCATCGAGCGCGGCAAGTTGTG
GATGTTGCAGACCCGCTCGGGCAAGCGCACCGCCAAGGCGGCGCTCAAGATTGCCGTGGAAATGGCAAGGGACGGCCTGA
TCACGAAGGAGGAAGCCGTCGCTCGCATCGACCCGGCCTCGCTCGACCAATTGCTGCACCCGACCATCGACCCGAAGGCC
GCGCGCGACGTCATCGGCGTCGGCCTGCCGGCATCGCCTGGCGCTGCCACTGGCGAGATCGTCTTTTCCTCCGGCGATGC
CGAGGATCTCAAGGCCCAGGGCCGCAAGGCGATCCTGGTGCGCATCGAGACCAGCCCGGAGGATATCCACGGCATGCATG
CGGCCGAGGGCATCCTGACCACGCGCGGCGGGATGACCAGCCATGCCGCCGTCGTCGCGCGCGGCATGGGCAAGCCTTGC
GTGTCCGGCGCCGGCTCACTGCGCGTCGACTACAAGGCCGGCACGCTGCTCTCGATGGGGCAGACCTTCCGCAAGGGCGA
CATCATCACCATCGACGGCGGCAACGGCCAGGTGCTGAAGGGCGCCGTGGCGATGCTGCAGCCTGAACTTTCCGGCGATT
TTGCCGCTATCATGGAATGGGCGGACGCGGTGCGGCGCATGAAGGTGCGCACAAACGCCGAAACCCCGCTCGATGCCCGC
ATGGCACGCTCTTTCGGCGCCGAGGGCATCGGTCTTTGCCGCACCGAGCACATGTTCTTCGACGGCGCCCGCATCGTCGC
CATGCGCGAGATGATCCTGGCCGATACCGAAAAGGATCGGCGCATCGCCCTTGCCAAGCTTCTGCCCATGCAGCGCTCGG
ATTTCCTGGAATTGTTCGAGATCATGGCAGGCCTGCCGGTGACGATCCGCCTGCTCGATCCGCCGTTGCATGAATTCCTG
CCCAAGACCGAGGCCGAAATCGCCGAGGTCGCGGCCGTCATGGATGTATCGCCCGACAAGCTCAGGCAGCGCACCGAGGC
CTTGCACGAATTCAATCCGATGCTTGGCCATCGCGGCTGCCGGCTGGCCGTCTCCTATCCCGAAATCGCCGAGATGCAGG
CACGCGCCATTTTCGAGGCCGCCGTCGAGGCAGGCCAGAAGGCTGGCGCGCTGGTCGTCCCCGAAATCATGGTGCCGCTG
GTCGGCCTGGTGAAGGAGCTCGACTATGTGAAGGCGCGCATCGATGCGGTCGCCAAGAGCGTCATGGAAGAGAGCGGCGT
CAAGATCGATTATCTCACCGGTACGATGATCGAACTGCCGCGCGCGGCGATCCGTGCCCATGTCATCGCCGCAACAGCCG
AGTTCTTCTCCTTCGGTACCAACGACCTCACCCAGACCACGTTCGGCATCTCGCGCGACGATGCGGCCTCGTTCCTGGAG
ACCTACCGCCAGAAAGGCATCATCGAGCAGGATCCATTCGTCTCGCTCGACGTCGATGGCGTCGGCGAACTGGTGCGCAT
GGCCGCGCAAAAGGGCAGGGCGACACGCCCCGACATCAAGCTCGGCATCTGCGGCGAACATGGCGGCGACCCGGCATCGA
TCCGCTTCTGCGAGGAGGTCGGGCTCGACTATGTCTCGTGCTCACCCTATCGCGTGCCGATCGCCAGGCTGGCGGCCGCG
CAGGCCGCGGTACAAGTGTCAAAAGCGGGGCGCGGGTAG

Upstream 100 bases:

>100_bases
TGCCTTTCGCACTGCAGCATCATATCGCTCCAAAGTCGGACCAGGGTGGAACTTTCCGTTTCGCCAATCTTTCCAATCTC
CTGCAACCGGAGAGCAAAGC

Downstream 100 bases:

>100_bases
GGAGATCAAGGCATTGCGCAATCGCACCGCGGTGAAACGGGAACGCGCTGTCTGAAGGGCGACTGATCGTCAGGCTGGCG
AATGGTGCGTGCGCCAGGGC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 892; Mature: 891

Protein sequence:

>892_residues
MTKWVFTFGDGAAEGRAGDKNLLGGKGANLAEMCSLGLPVPPGFTITTEVCNAYYANGRAYPAGLEADVAVALDHIGRLT
DRRFGDPSKLLLVSVRSGARASMPGMMDTVLNLGLNDETVEALATDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEIL
EDQKASLGHELDTELTAIEWQGVIALYKAKVEEELGRPFPQDPQEQLWGAIGAVFSSWMNNRAITYRRLHDIPESWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFLVNAQGEDVVAGIRTPQNITEAARIAAGSDKPSLQKLMPDAFQSF
VSISDSLEKHYRDMQDLEFTIERGKLWMLQTRSGKRTAKAALKIAVEMARDGLITKEEAVARIDPASLDQLLHPTIDPKA
ARDVIGVGLPASPGAATGEIVFSSGDAEDLKAQGRKAILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSLRVDYKAGTLLSMGQTFRKGDIITIDGGNGQVLKGAVAMLQPELSGDFAAIMEWADAVRRMKVRTNAETPLDAR
MARSFGAEGIGLCRTEHMFFDGARIVAMREMILADTEKDRRIALAKLLPMQRSDFLELFEIMAGLPVTIRLLDPPLHEFL
PKTEAEIAEVAAVMDVSPDKLRQRTEALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGQKAGALVVPEIMVPL
VGLVKELDYVKARIDAVAKSVMEESGVKIDYLTGTMIELPRAAIRAHVIAATAEFFSFGTNDLTQTTFGISRDDAASFLE
TYRQKGIIEQDPFVSLDVDGVGELVRMAAQKGRATRPDIKLGICGEHGGDPASIRFCEEVGLDYVSCSPYRVPIARLAAA
QAAVQVSKAGRG

Sequences:

>Translated_892_residues
MTKWVFTFGDGAAEGRAGDKNLLGGKGANLAEMCSLGLPVPPGFTITTEVCNAYYANGRAYPAGLEADVAVALDHIGRLT
DRRFGDPSKLLLVSVRSGARASMPGMMDTVLNLGLNDETVEALATDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEIL
EDQKASLGHELDTELTAIEWQGVIALYKAKVEEELGRPFPQDPQEQLWGAIGAVFSSWMNNRAITYRRLHDIPESWGTAV
NVQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFLVNAQGEDVVAGIRTPQNITEAARIAAGSDKPSLQKLMPDAFQSF
VSISDSLEKHYRDMQDLEFTIERGKLWMLQTRSGKRTAKAALKIAVEMARDGLITKEEAVARIDPASLDQLLHPTIDPKA
ARDVIGVGLPASPGAATGEIVFSSGDAEDLKAQGRKAILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSLRVDYKAGTLLSMGQTFRKGDIITIDGGNGQVLKGAVAMLQPELSGDFAAIMEWADAVRRMKVRTNAETPLDAR
MARSFGAEGIGLCRTEHMFFDGARIVAMREMILADTEKDRRIALAKLLPMQRSDFLELFEIMAGLPVTIRLLDPPLHEFL
PKTEAEIAEVAAVMDVSPDKLRQRTEALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGQKAGALVVPEIMVPL
VGLVKELDYVKARIDAVAKSVMEESGVKIDYLTGTMIELPRAAIRAHVIAATAEFFSFGTNDLTQTTFGISRDDAASFLE
TYRQKGIIEQDPFVSLDVDGVGELVRMAAQKGRATRPDIKLGICGEHGGDPASIRFCEEVGLDYVSCSPYRVPIARLAAA
QAAVQVSKAGRG
>Mature_891_residues
TKWVFTFGDGAAEGRAGDKNLLGGKGANLAEMCSLGLPVPPGFTITTEVCNAYYANGRAYPAGLEADVAVALDHIGRLTD
RRFGDPSKLLLVSVRSGARASMPGMMDTVLNLGLNDETVEALATDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEILE
DQKASLGHELDTELTAIEWQGVIALYKAKVEEELGRPFPQDPQEQLWGAIGAVFSSWMNNRAITYRRLHDIPESWGTAVN
VQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFLVNAQGEDVVAGIRTPQNITEAARIAAGSDKPSLQKLMPDAFQSFV
SISDSLEKHYRDMQDLEFTIERGKLWMLQTRSGKRTAKAALKIAVEMARDGLITKEEAVARIDPASLDQLLHPTIDPKAA
RDVIGVGLPASPGAATGEIVFSSGDAEDLKAQGRKAILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPCV
SGAGSLRVDYKAGTLLSMGQTFRKGDIITIDGGNGQVLKGAVAMLQPELSGDFAAIMEWADAVRRMKVRTNAETPLDARM
ARSFGAEGIGLCRTEHMFFDGARIVAMREMILADTEKDRRIALAKLLPMQRSDFLELFEIMAGLPVTIRLLDPPLHEFLP
KTEAEIAEVAAVMDVSPDKLRQRTEALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGQKAGALVVPEIMVPLV
GLVKELDYVKARIDAVAKSVMEESGVKIDYLTGTMIELPRAAIRAHVIAATAEFFSFGTNDLTQTTFGISRDDAASFLET
YRQKGIIEQDPFVSLDVDGVGELVRMAAQKGRATRPDIKLGICGEHGGDPASIRFCEEVGLDYVSCSPYRVPIARLAAAQ
AAVQVSKAGRG

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=463, Percent_Identity=25.4859611231102, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI48994992, Length=442, Percent_Identity=25.3393665158371, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789193, Length=432, Percent_Identity=25.6944444444444, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1788726, Length=431, Percent_Identity=24.5939675174014, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96491; Mature: 96359

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVFTFGDGAAEGRAGDKNLLGGKGANLAEMCSLGLPVPPGFTITTEVCNAYYANGRA
CCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPAGLEADVAVALDHIGRLTDRRFGDPSKLLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EALATDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEILEDQKASLGHELDTELTAIEW
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
QGVIALYKAKVEEELGRPFPQDPQEQLWGAIGAVFSSWMNNRAITYRRLHDIPESWGTAV
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCHHHHCCEE
NVQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFLVNAQGEDVVAGIRTPQNITEAARI
EEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
AAGSDKPSLQKLMPDAFQSFVSISDSLEKHYRDMQDLEFTIERGKLWMLQTRSGKRTAKA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCHHHHH
ALKIAVEMARDGLITKEEAVARIDPASLDQLLHPTIDPKAARDVIGVGLPASPGAATGEI
HHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEE
VFSSGDAEDLKAQGRKAILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EEECCCHHHHHHCCCEEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCCC
VSGAGSLRVDYKAGTLLSMGQTFRKGDIITIDGGNGQVLKGAVAMLQPELSGDFAAIMEW
CCCCCCEEEEECCCCHHHHCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHH
ADAVRRMKVRTNAETPLDARMARSFGAEGIGLCRTEHMFFDGARIVAMREMILADTEKDR
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCCCHH
RIALAKLLPMQRSDFLELFEIMAGLPVTIRLLDPPLHEFLPKTEAEIAEVAAVMDVSPDK
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
LRQRTEALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGQKAGALVVPEIMVPL
HHHHHHHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHH
VGLVKELDYVKARIDAVAKSVMEESGVKIDYLTGTMIELPRAAIRAHVIAATAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTTFGISRDDAASFLETYRQKGIIEQDPFVSLDVDGVGELVRMAAQKGRATRPDIK
CCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEE
LGICGEHGGDPASIRFCEEVGLDYVSCSPYRVPIARLAAAQAAVQVSKAGRG
EEECCCCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKWVFTFGDGAAEGRAGDKNLLGGKGANLAEMCSLGLPVPPGFTITTEVCNAYYANGRA
CEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCC
YPAGLEADVAVALDHIGRLTDRRFGDPSKLLLVSVRSGARASMPGMMDTVLNLGLNDETV
CCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
EALATDSGDARFAYDSYRRFIQMYSDVVMGLDHEVFEEILEDQKASLGHELDTELTAIEW
HHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEC
QGVIALYKAKVEEELGRPFPQDPQEQLWGAIGAVFSSWMNNRAITYRRLHDIPESWGTAV
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCHHHHCCEE
NVQAMVFGNMGETSATGVAFTRNPSTGEKQLYGEFLVNAQGEDVVAGIRTPQNITEAARI
EEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHH
AAGSDKPSLQKLMPDAFQSFVSISDSLEKHYRDMQDLEFTIERGKLWMLQTRSGKRTAKA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEECCCCHHHHH
ALKIAVEMARDGLITKEEAVARIDPASLDQLLHPTIDPKAARDVIGVGLPASPGAATGEI
HHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEE
VFSSGDAEDLKAQGRKAILVRIETSPEDIHGMHAAEGILTTRGGMTSHAAVVARGMGKPC
EEECCCHHHHHHCCCEEEEEEEECCCHHHCCHHHHCCCEEECCCCCHHHHHHHHCCCCCC
VSGAGSLRVDYKAGTLLSMGQTFRKGDIITIDGGNGQVLKGAVAMLQPELSGDFAAIMEW
CCCCCCEEEEECCCCHHHHCHHHCCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHH
ADAVRRMKVRTNAETPLDARMARSFGAEGIGLCRTEHMFFDGARIVAMREMILADTEKDR
HHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEECCEEECCHHHHHHHHHHHHCCCCHH
RIALAKLLPMQRSDFLELFEIMAGLPVTIRLLDPPLHEFLPKTEAEIAEVAAVMDVSPDK
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHH
LRQRTEALHEFNPMLGHRGCRLAVSYPEIAEMQARAIFEAAVEAGQKAGALVVPEIMVPL
HHHHHHHHHHHCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHH
VGLVKELDYVKARIDAVAKSVMEESGVKIDYLTGTMIELPRAAIRAHVIAATAEFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCC
NDLTQTTFGISRDDAASFLETYRQKGIIEQDPFVSLDVDGVGELVRMAAQKGRATRPDIK
CCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEE
LGICGEHGGDPASIRFCEEVGLDYVSCSPYRVPIARLAAAQAAVQVSKAGRG
EEECCCCCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]