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The map label for this gene is lutR [H]

Identifier: 13476140

GI number: 13476140

Start: 6104008

End: 6104682

Strand: Reverse

Name: lutR [H]

Synonym: mll7378

Alternate gene names: 13476140

Gene position: 6104682-6104008 (Counterclockwise)

Preceding gene: 13476148

Following gene: 13476139

Centisome position: 86.76

GC content: 61.48

Gene sequence:

>675_bases
GTGAGCGCCGCCATTCAGGCGATCACCCAACACATCCGTGTTGAAGGCCTTGGCCCTGGCGACCTCTTGCCCAGTGAAGC
CGACATGACCCGCAAGCTCAGTGTCTCGCGGACGGTGGTGCGGGAGGCGTTTCGATCGCTGTCGGCGATGCGTCTCATCG
ACATGAGCGCCGGGCGGCGCGCGAGCGTGGCCAAGCTCGACATTGGCGCCATGTCGATGGTGATCGAGCACGGCGTCACG
ACCGAACAGATCAGCGTCCAACAGGTTTATGATGTGCGCCGCACCATCGAAATGCGCACGGTGGCGCTCGCAGCACTACG
CCGGACCGACGCGGAGGCGGCTGAAATACAGAAATGCGCGCAGCTGATGCGCGAGAATTACCGTGCGCCCGAGATCGTCA
TGGAACACGACATCGCATTCCATGAAGCGATCGCGCGGGCCTCTCACAACCCGGTGTTTTCGTTGATCATCAAGGCCTTC
AGCGGTGTCACGCGACGCACCTGGGTGATCGGCTGGCGTACGCGTGCAACCGAGGAAGAGCAGATCAAGATGATCGAGGG
GCACGCAGATATTGCTCGGGCGATCATCGCGGGGAACCCGCAACTTGCCGCCGAACATATGGCGGCGCATTTCGATAAAA
GCGTCAAGGCATTGATCGACGCGGGGATCGCATGA

Upstream 100 bases:

>100_bases
TTCACAAAGCTATAGGCATTTCAATAAAACATGTCATACAGGTTTGGGAAAAATGGTAAGCGATCGCGGCATAGGGCCTG
ACGAGACAAATCCCGGGCTG

Downstream 100 bases:

>100_bases
AAATCCACTCGCTTGAGACCATTCGTATCGAAGAGCGGCCCAATCTGCTCTGGATCGAGGTGCATACCGACGAGGGCATC
ACCGGCCTTGGCGAAACCTT

Product: transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSAAIQAITQHIRVEGLGPGDLLPSEADMTRKLSVSRTVVREAFRSLSAMRLIDMSAGRRASVAKLDIGAMSMVIEHGVT
TEQISVQQVYDVRRTIEMRTVALAALRRTDAEAAEIQKCAQLMRENYRAPEIVMEHDIAFHEAIARASHNPVFSLIIKAF
SGVTRRTWVIGWRTRATEEEQIKMIEGHADIARAIIAGNPQLAAEHMAAHFDKSVKALIDAGIA

Sequences:

>Translated_224_residues
MSAAIQAITQHIRVEGLGPGDLLPSEADMTRKLSVSRTVVREAFRSLSAMRLIDMSAGRRASVAKLDIGAMSMVIEHGVT
TEQISVQQVYDVRRTIEMRTVALAALRRTDAEAAEIQKCAQLMRENYRAPEIVMEHDIAFHEAIARASHNPVFSLIIKAF
SGVTRRTWVIGWRTRATEEEQIKMIEGHADIARAIIAGNPQLAAEHMAAHFDKSVKALIDAGIA
>Mature_223_residues
SAAIQAITQHIRVEGLGPGDLLPSEADMTRKLSVSRTVVREAFRSLSAMRLIDMSAGRRASVAKLDIGAMSMVIEHGVTT
EQISVQQVYDVRRTIEMRTVALAALRRTDAEAAEIQKCAQLMRENYRAPEIVMEHDIAFHEAIARASHNPVFSLIIKAFS
GVTRRTWVIGWRTRATEEEQIKMIEGHADIARAIIAGNPQLAAEHMAAHFDKSVKALIDAGIA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994955, Length=161, Percent_Identity=35.4037267080745, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789353, Length=211, Percent_Identity=28.9099526066351, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24695; Mature: 24564

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIQAITQHIRVEGLGPGDLLPSEADMTRKLSVSRTVVREAFRSLSAMRLIDMSAGRR
CCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASVAKLDIGAMSMVIEHGVTTEQISVQQVYDVRRTIEMRTVALAALRRTDAEAAEIQKCA
CCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLMRENYRAPEIVMEHDIAFHEAIARASHNPVFSLIIKAFSGVTRRTWVIGWRTRATEEE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEEECCCCCCHH
QIKMIEGHADIARAIIAGNPQLAAEHMAAHFDKSVKALIDAGIA
HHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAAIQAITQHIRVEGLGPGDLLPSEADMTRKLSVSRTVVREAFRSLSAMRLIDMSAGRR
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASVAKLDIGAMSMVIEHGVTTEQISVQQVYDVRRTIEMRTVALAALRRTDAEAAEIQKCA
CCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLMRENYRAPEIVMEHDIAFHEAIARASHNPVFSLIIKAFSGVTRRTWVIGWRTRATEEE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEEECCCCCCHH
QIKMIEGHADIARAIIAGNPQLAAEHMAAHFDKSVKALIDAGIA
HHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]