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The map label for this gene is tpiA

Identifier: 13476061

GI number: 13476061

Start: 6013696

End: 6014460

Strand: Direct

Name: tpiA

Synonym: mlr7275

Alternate gene names: 13476061

Gene position: 6013696-6014460 (Clockwise)

Preceding gene: 13476060

Following gene: 13476062

Centisome position: 85.47

GC content: 65.62

Gene sequence:

>765_bases
GTGCGCGTGGTCTACTGGGTCGGTACAAGCTGGAAGATGAACAAGACGCTCGCCGAGGCACTGGATTTCGCGGAGCGCCT
GGCAGGCTTCATTCCCGGTTTCGATGACCGCATCCAGCCCTTCGTCATCCCACCTTTCACGGCGGTGCGCGAGGTCAAGC
GGGCGCTGTCGTCGACGCGCATCAAGGTCGGTGCGCAGAACATGCATTGGGCCGATGCCGGCGCCTGGACCGGCGAGATT
TCGCCACCGATGCTGAGGGATTGCGGGCTCGATCTGGTCGAACTCGGCCACAGCGAACGGCGCGAGCATTTTGGCGAGAC
CGATCGCACCGTCGGCCTGAAAACGGCTGCCGCCGTGAAGCACGGGATGATACCGCTGATCTGTGTCGGCGAGACGCTGG
CCGAGCGCGAAAGTGGGCAGGCCGATGCCGTGCTGAGCGCCCAGGTCGAGGGCGCGCTGCAGTTCCTCGAAGGCGAGGCG
AAGACCGCGAAAATCCTGTTCGCCTACGAGCCGGTCTGGGCGATCGGCGACAAGGGCATTCCGGCCAGCGCCGACTATGC
CGACAAGCAGCAGGCATTGATCAAGACAGTGGCCGGCGCCCTGCTGCCGTCGGTGCCGCCGGTGCTTTATGGCGGCAGCG
TCAATCCCGGCAATGCCGCCGAGCTGGTCGGCCAGCCCAATATAGACGGCCTCTTCATCGGCCGTTCCGCCTGGCAGGCG
GACGGCTACATCGACATTCTCGGCCGCGCCTCGGCGGCGATCTGA

Upstream 100 bases:

>100_bases
ACGCGCGGCGCTGGAGGCCGCCGGCGTTTCGCTGACGGTGATGGACAAGGGATCATGACCCCTGAAATCGGCAGATCGTG
ATCAACAAAGTCAAGGAGCA

Downstream 100 bases:

>100_bases
AACCATCCGGACACGAAAAGGGAAAAACATGAAAATAGCCATTGGAGCCGACAGCGCCGGCAAGCCGCTGCTCGATGTCA
TCGCCGCGCACCTTGCCACC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRVVYWVGTSWKMNKTLAEALDFAERLAGFIPGFDDRIQPFVIPPFTAVREVKRALSSTRIKVGAQNMHWADAGAWTGEI
SPPMLRDCGLDLVELGHSERREHFGETDRTVGLKTAAAVKHGMIPLICVGETLAERESGQADAVLSAQVEGALQFLEGEA
KTAKILFAYEPVWAIGDKGIPASADYADKQQALIKTVAGALLPSVPPVLYGGSVNPGNAAELVGQPNIDGLFIGRSAWQA
DGYIDILGRASAAI

Sequences:

>Translated_254_residues
MRVVYWVGTSWKMNKTLAEALDFAERLAGFIPGFDDRIQPFVIPPFTAVREVKRALSSTRIKVGAQNMHWADAGAWTGEI
SPPMLRDCGLDLVELGHSERREHFGETDRTVGLKTAAAVKHGMIPLICVGETLAERESGQADAVLSAQVEGALQFLEGEA
KTAKILFAYEPVWAIGDKGIPASADYADKQQALIKTVAGALLPSVPPVLYGGSVNPGNAAELVGQPNIDGLFIGRSAWQA
DGYIDILGRASAAI
>Mature_254_residues
MRVVYWVGTSWKMNKTLAEALDFAERLAGFIPGFDDRIQPFVIPPFTAVREVKRALSSTRIKVGAQNMHWADAGAWTGEI
SPPMLRDCGLDLVELGHSERREHFGETDRTVGLKTAAAVKHGMIPLICVGETLAERESGQADAVLSAQVEGALQFLEGEA
KTAKILFAYEPVWAIGDKGIPASADYADKQQALIKTVAGALLPSVPPVLYGGSVNPGNAAELVGQPNIDGLFIGRSAWQA
DGYIDILGRASAAI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=34.9397590361446, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=35.3413654618474, Blast_Score=136, Evalue=2e-32,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=35.6275303643725, Blast_Score=139, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17536593, Length=257, Percent_Identity=35.408560311284, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320255, Length=254, Percent_Identity=32.2834645669291, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572008, Length=252, Percent_Identity=34.5238095238095, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28572006, Length=252, Percent_Identity=34.5238095238095, Blast_Score=131, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28572004, Length=252, Percent_Identity=34.5238095238095, Blast_Score=130, Evalue=6e-31,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS2_RHILO (Q986N6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_107631.1
- ProteinModelPortal:   Q986N6
- SMR:   Q986N6
- GeneID:   1230286
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr7275
- NMPDR:   fig|266835.1.peg.5732
- HOGENOM:   HBG708281
- OMA:   AWTGEVS
- ProtClustDB:   PRK00042
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27199; Mature: 27199

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 97-97 ACT_SITE 170-170 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVYWVGTSWKMNKTLAEALDFAERLAGFIPGFDDRIQPFVIPPFTAVREVKRALSSTR
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHCE
IKVGAQNMHWADAGAWTGEISPPMLRDCGLDLVELGHSERREHFGETDRTVGLKTAAAVK
EEECCCCCEECCCCCCCCCCCCHHHHHCCCHHHHCCCHHHHHHCCCCCCEECHHHHHHHH
HGMIPLICVGETLAERESGQADAVLSAQVEGALQFLEGEAKTAKILFAYEPVWAIGDKGI
HCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCCC
PASADYADKQQALIKTVAGALLPSVPPVLYGGSVNPGNAAELVGQPNIDGLFIGRSAWQA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCC
DGYIDILGRASAAI
CCEEEEECCCCCCC
>Mature Secondary Structure
MRVVYWVGTSWKMNKTLAEALDFAERLAGFIPGFDDRIQPFVIPPFTAVREVKRALSSTR
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHCE
IKVGAQNMHWADAGAWTGEISPPMLRDCGLDLVELGHSERREHFGETDRTVGLKTAAAVK
EEECCCCCEECCCCCCCCCCCCHHHHHCCCHHHHCCCHHHHHHCCCCCCEECHHHHHHHH
HGMIPLICVGETLAERESGQADAVLSAQVEGALQFLEGEAKTAKILFAYEPVWAIGDKGI
HCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEECCCCC
PASADYADKQQALIKTVAGALLPSVPPVLYGGSVNPGNAAELVGQPNIDGLFIGRSAWQA
CCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHCCCCCCCEEEEECCCCCC
DGYIDILGRASAAI
CCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968