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The map label for this gene is dctD [H]

Identifier: 13476031

GI number: 13476031

Start: 5978831

End: 5980213

Strand: Direct

Name: dctD [H]

Synonym: mlr7239

Alternate gene names: 13476031

Gene position: 5978831-5980213 (Clockwise)

Preceding gene: 13476030

Following gene: 13476032

Centisome position: 84.97

GC content: 66.31

Gene sequence:

>1383_bases
ATGAACGAGGGATCACGGAGGGTGGCGCTCGTCGAGGACGATGCCGACCTGCTGCACGCCACCACGCAATTGCTCGAGCT
CGCCGGTTTCACCGTGGTTGCGCGCGAGGCCGCCGAGGCCGCATTTGCCGTCGTCGACAGAGACTTCGACGGCGTGGTTG
TCACCGACATCCGCATGCCGGGCATGAACGGCTTGCAGCTGTTCGATCGCATTAAGGCGATCGACCCGGACATACCGGTG
ATCCTGGTCACCGGGCATGGCGATGTCGATCTGGCTGTCGCCGCGCTCAAGGACGGTGTCTACGACTTCATCCCAAAACC
CTATGCCGGAGACCGCCTTGTCGAGGCGCTGAAACGGGCATCGGAGAAGCGGCGGCTGGTGATGGAGAACCGGCGGCTGC
GCGAAGCCGCCGCACTAGCTGCCGACGGCCTGCCGCTGATCGGCGAGGCGCCGGCCATCCGGCGGCTGCGCGAAACCTTG
CGCCAGATCGCCGACATGGATGTCGACGTGCTGGTGGAAGGCGAGACGGGAACCGGCAAGGAAGTGGTGGCCGACCTCCT
GCACCGTTGGGGCCGGCGGCATTCCAAACCCTTCGTGGCGCTGAATTGCGGTGCCTTGCCGGAAACCGTCATTGAAAGCG
AGCTGTTCGGCCACGAGGCCGGCGCGTTTACCGGCGCGCAGCGGCGGCGGATCGGCCGCGTCGAACATTCCAGCGGCGGC
ACGCTGTTCCTCGACGAGATCGAATCCATGCCGCCGGCCCTGCAGGTCAAGCTGCTGCGGGTGCTGGAGACGCGCAGCCT
CACGCCGCTTGGCTCGAACGAGATCCGGCGCCTCGATCTGAGGGTCGTGGCGGCGACTAAGGTCGATCTCGGCCGGCCCG
ATCAGCGCGGCGATTTCCGTGAGGACCTCTATTTCCGCCTCAACGTGGTGACGCTACGGATTCCGCCCCTGCGCGAGCGG
CGCGGCGACATTCCGATGCTGTTCGGCCATTTCCTCGGTAAGGCCGCCGAGCGGTTCGGCCGGCCTGTCGCCAAGGTGAA
TGCCGCGGTGAGCGATCATCTCCAGTCGCATGCCTGGCCGGGCAATGTCCGCGAGCTCGCCCATTTCGCCGATCGCGTCA
CGCTCGGTCTTGGGCCTGACGACGAGACGAAAGCGGTTCCCCCGCAATCGGAGCCTCCCGCCTCGCTGCCGGAACGCGTG
GGCCATTACGAGGCGCAGCTCATTCGTGAGGCGCTGCGCGACCATGGCGGCGATGTGCGCAGCGCCATCGACGCGCTCGG
CGTGCCGCGCAAGACTTTTTACGACAAGCTCAAGCGCTACGGCATTGCCGCATCCGAATTCCGCAACGGCGGCGATCCGC
ACCCTGTTCGAGAGCAGACATAG

Upstream 100 bases:

>100_bases
TGATCTCGCAGGAGATCGCCCAGGAATTGGGCGGCACGCTCCGGCTCGATCCCGACAGCACCAGAGGCGCGTCCTTCACC
ATCGATTTGAGGCGAATCGA

Downstream 100 bases:

>100_bases
AAATTGCGCCAAAGGTGTTGCGCTTCGCAACGCTTCAATCCTACCCAGCCAAGCATTCCAGAAAGAGCCATGAACACGAC
ACTCGAAACGACGGCAAACC

Product: two-component C4-dicarboxylate transport system, regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNEGSRRVALVEDDADLLHATTQLLELAGFTVVAREAAEAAFAVVDRDFDGVVVTDIRMPGMNGLQLFDRIKAIDPDIPV
ILVTGHGDVDLAVAALKDGVYDFIPKPYAGDRLVEALKRASEKRRLVMENRRLREAAALAADGLPLIGEAPAIRRLRETL
RQIADMDVDVLVEGETGTGKEVVADLLHRWGRRHSKPFVALNCGALPETVIESELFGHEAGAFTGAQRRRIGRVEHSSGG
TLFLDEIESMPPALQVKLLRVLETRSLTPLGSNEIRRLDLRVVAATKVDLGRPDQRGDFREDLYFRLNVVTLRIPPLRER
RGDIPMLFGHFLGKAAERFGRPVAKVNAAVSDHLQSHAWPGNVRELAHFADRVTLGLGPDDETKAVPPQSEPPASLPERV
GHYEAQLIREALRDHGGDVRSAIDALGVPRKTFYDKLKRYGIAASEFRNGGDPHPVREQT

Sequences:

>Translated_460_residues
MNEGSRRVALVEDDADLLHATTQLLELAGFTVVAREAAEAAFAVVDRDFDGVVVTDIRMPGMNGLQLFDRIKAIDPDIPV
ILVTGHGDVDLAVAALKDGVYDFIPKPYAGDRLVEALKRASEKRRLVMENRRLREAAALAADGLPLIGEAPAIRRLRETL
RQIADMDVDVLVEGETGTGKEVVADLLHRWGRRHSKPFVALNCGALPETVIESELFGHEAGAFTGAQRRRIGRVEHSSGG
TLFLDEIESMPPALQVKLLRVLETRSLTPLGSNEIRRLDLRVVAATKVDLGRPDQRGDFREDLYFRLNVVTLRIPPLRER
RGDIPMLFGHFLGKAAERFGRPVAKVNAAVSDHLQSHAWPGNVRELAHFADRVTLGLGPDDETKAVPPQSEPPASLPERV
GHYEAQLIREALRDHGGDVRSAIDALGVPRKTFYDKLKRYGIAASEFRNGGDPHPVREQT
>Mature_460_residues
MNEGSRRVALVEDDADLLHATTQLLELAGFTVVAREAAEAAFAVVDRDFDGVVVTDIRMPGMNGLQLFDRIKAIDPDIPV
ILVTGHGDVDLAVAALKDGVYDFIPKPYAGDRLVEALKRASEKRRLVMENRRLREAAALAADGLPLIGEAPAIRRLRETL
RQIADMDVDVLVEGETGTGKEVVADLLHRWGRRHSKPFVALNCGALPETVIESELFGHEAGAFTGAQRRRIGRVEHSSGG
TLFLDEIESMPPALQVKLLRVLETRSLTPLGSNEIRRLDLRVVAATKVDLGRPDQRGDFREDLYFRLNVVTLRIPPLRER
RGDIPMLFGHFLGKAAERFGRPVAKVNAAVSDHLQSHAWPGNVRELAHFADRVTLGLGPDDETKAVPPQSEPPASLPERV
GHYEAQLIREALRDHGGDVRSAIDALGVPRKTFYDKLKRYGIAASEFRNGGDPHPVREQT

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=460, Percent_Identity=40.2173913043478, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788550, Length=451, Percent_Identity=35.920177383592, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=39.1799544419134, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790299, Length=467, Percent_Identity=38.1156316916488, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789087, Length=333, Percent_Identity=40.5405405405405, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=39.937106918239, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=38.7931034482759, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1786524, Length=313, Percent_Identity=39.9361022364217, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082152, Length=230, Percent_Identity=46.0869565217391, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=36.7924528301887, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787583, Length=333, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=31.1897106109325, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789828, Length=271, Percent_Identity=33.5793357933579, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=30.1724137931034, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50708; Mature: 50708

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEGSRRVALVEDDADLLHATTQLLELAGFTVVAREAAEAAFAVVDRDFDGVVVTDIRMP
CCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
GMNGLQLFDRIKAIDPDIPVILVTGHGDVDLAVAALKDGVYDFIPKPYAGDRLVEALKRA
CCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHH
SEKRRLVMENRRLREAAALAADGLPLIGEAPAIRRLRETLRQIADMDVDVLVEGETGTGK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCH
EVVADLLHRWGRRHSKPFVALNCGALPETVIESELFGHEAGAFTGAQRRRIGRVEHSSGG
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCC
TLFLDEIESMPPALQVKLLRVLETRSLTPLGSNEIRRLDLRVVAATKVDLGRPDQRGDFR
EEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHH
EDLYFRLNVVTLRIPPLRERRGDIPMLFGHFLGKAAERFGRPVAKVNAAVSDHLQSHAWP
HCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GNVRELAHFADRVTLGLGPDDETKAVPPQSEPPASLPERVGHYEAQLIREALRDHGGDVR
CHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
SAIDALGVPRKTFYDKLKRYGIAASEFRNGGDPHPVREQT
HHHHHHCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNEGSRRVALVEDDADLLHATTQLLELAGFTVVAREAAEAAFAVVDRDFDGVVVTDIRMP
CCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
GMNGLQLFDRIKAIDPDIPVILVTGHGDVDLAVAALKDGVYDFIPKPYAGDRLVEALKRA
CCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHH
SEKRRLVMENRRLREAAALAADGLPLIGEAPAIRRLRETLRQIADMDVDVLVEGETGTGK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCH
EVVADLLHRWGRRHSKPFVALNCGALPETVIESELFGHEAGAFTGAQRRRIGRVEHSSGG
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCC
TLFLDEIESMPPALQVKLLRVLETRSLTPLGSNEIRRLDLRVVAATKVDLGRPDQRGDFR
EEEHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHH
EDLYFRLNVVTLRIPPLRERRGDIPMLFGHFLGKAAERFGRPVAKVNAAVSDHLQSHAWP
HCCEEEEEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
GNVRELAHFADRVTLGLGPDDETKAVPPQSEPPASLPERVGHYEAQLIREALRDHGGDVR
CHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
SAIDALGVPRKTFYDKLKRYGIAASEFRNGGDPHPVREQT
HHHHHHCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]