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The map label for this gene is iolE [H]

Identifier: 13476022

GI number: 13476022

Start: 5969768

End: 5970589

Strand: Direct

Name: iolE [H]

Synonym: mlr7228

Alternate gene names: 13476022

Gene position: 5969768-5970589 (Clockwise)

Preceding gene: 13476021

Following gene: 13476023

Centisome position: 84.85

GC content: 64.72

Gene sequence:

>822_bases
ATGAACGTGAAGGACCTTAAAGTCGGCTGCCAGACCTTTACCTGGGAAATGCTCGGCGACCGTTTTGCCGGCGGCCCCGA
CGATCTGTTGAAGGCGATTGCCGACGGCGGCTATTCCGGCATCGAGATCACCGACACCATGATCGGCCGCTACGCCGACC
GGCCGTCGGAATTCGCCGCCGCGCTGAAGTCGTCCGGCCTGACGCTGGTCTCCTTTGCCTTCGGCTCGAACAGCGGCTTT
ACGCTGAAAGAGGAGATCGGCGCCGACCTCGAGGCGGCACAGCGCTGGATCGATTTCGCCGCCGCCTTTCCCGGCGCGCT
GGTGTCCATGGGTTCGGCAACCGTGGTGTCCGACGGGCCGCGCGAAGGCAAGTTCGCCATCGCCGCCGAGGTCTACAACA
GGGCCGGCGAACTCGGCCGCAAGGCCGGTGTCCAGGTGGCGGTGCATCCGAGTTCGCACCACAACACGCTGCTGTTCGAC
CGCGCCGACTACGACAGGATCTTCGCCTTGCTCGACCGCGATCTGATCGGCTGGGTGCCGGATACTGGCCATATTTTGCG
CGGCCACAAGGACATGGCCGACACACTGCGCACCTATCGCGACCGAATCCGCTACATCCATCTGAAGGACGTCGACGCCA
ACGGCACCTGGGCGATGCTGGGCAAGGGGGTCTGCGATACCCATGCAGTTATCGAGATTGCCGGCACCGCGCCCCGCTTC
AATGGCTGGCTGGTGCTGGAAGAGGAATCCGAAACGGCTGCCGCCGATCCCGCCGGCGCGGTCAAGACCAACCGCCAGAC
GATGCGCAGCTACGGCGCCTGA

Upstream 100 bases:

>100_bases
CTTCCTCGCCGGGATGACCGCAGGCTCGGTCAAGTAGGATGTCATTCCCACGGCGACGCAGCGTGGCATCAAGTAATAAG
CGGTATCAAGGATTGAAAGC

Downstream 100 bases:

>100_bases
AGAAACGAGACGATGATCAAGAAACAAGACAGACGCCTCAGGGTGGGTGTGCTCGGCTGCGGCCCGATCGCGCAGTTCGC
GCATCTGGAATCCTGCGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: 2-keto-myo-inositol dehydratase; 2KMI dehydratase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNVKDLKVGCQTFTWEMLGDRFAGGPDDLLKAIADGGYSGIEITDTMIGRYADRPSEFAAALKSSGLTLVSFAFGSNSGF
TLKEEIGADLEAAQRWIDFAAAFPGALVSMGSATVVSDGPREGKFAIAAEVYNRAGELGRKAGVQVAVHPSSHHNTLLFD
RADYDRIFALLDRDLIGWVPDTGHILRGHKDMADTLRTYRDRIRYIHLKDVDANGTWAMLGKGVCDTHAVIEIAGTAPRF
NGWLVLEEESETAAADPAGAVKTNRQTMRSYGA

Sequences:

>Translated_273_residues
MNVKDLKVGCQTFTWEMLGDRFAGGPDDLLKAIADGGYSGIEITDTMIGRYADRPSEFAAALKSSGLTLVSFAFGSNSGF
TLKEEIGADLEAAQRWIDFAAAFPGALVSMGSATVVSDGPREGKFAIAAEVYNRAGELGRKAGVQVAVHPSSHHNTLLFD
RADYDRIFALLDRDLIGWVPDTGHILRGHKDMADTLRTYRDRIRYIHLKDVDANGTWAMLGKGVCDTHAVIEIAGTAPRF
NGWLVLEEESETAAADPAGAVKTNRQTMRSYGA
>Mature_273_residues
MNVKDLKVGCQTFTWEMLGDRFAGGPDDLLKAIADGGYSGIEITDTMIGRYADRPSEFAAALKSSGLTLVSFAFGSNSGF
TLKEEIGADLEAAQRWIDFAAAFPGALVSMGSATVVSDGPREGKFAIAAEVYNRAGELGRKAGVQVAVHPSSHHNTLLFD
RADYDRIFALLDRDLIGWVPDTGHILRGHKDMADTLRTYRDRIRYIHLKDVDANGTWAMLGKGVCDTHAVIEIAGTAPRF
NGWLVLEEESETAAADPAGAVKTNRQTMRSYGA

Specific function: Catalyzes the dehydration of inosose (2-keto-myo- inositol, 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi- inositol) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iolE/mocC family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =4.2.1.44 [H]

Molecular weight: Translated: 29518; Mature: 29518

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKDLKVGCQTFTWEMLGDRFAGGPDDLLKAIADGGYSGIEITDTMIGRYADRPSEFAA
CCCCHHEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCHHHHHH
ALKSSGLTLVSFAFGSNSGFTLKEEIGADLEAAQRWIDFAAAFPGALVSMGSATVVSDGP
HHHHCCCEEEEEEECCCCCCEEHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCEEEECCCC
REGKFAIAAEVYNRAGELGRKAGVQVAVHPSSHHNTLLFDRADYDRIFALLDRDLIGWVP
CCCCEEEHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
DTGHILRGHKDMADTLRTYRDRIRYIHLKDVDANGTWAMLGKGVCDTHAVIEIAGTAPRF
CCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCC
NGWLVLEEESETAAADPAGAVKTNRQTMRSYGA
CCEEEEECCCCCCCCCCCCCHHCCHHHHHHCCC
>Mature Secondary Structure
MNVKDLKVGCQTFTWEMLGDRFAGGPDDLLKAIADGGYSGIEITDTMIGRYADRPSEFAA
CCCCHHEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHCCCHHHHHH
ALKSSGLTLVSFAFGSNSGFTLKEEIGADLEAAQRWIDFAAAFPGALVSMGSATVVSDGP
HHHHCCCEEEEEEECCCCCCEEHHHHCCCHHHHHHHHHHHHHCCHHHHHCCCEEEECCCC
REGKFAIAAEVYNRAGELGRKAGVQVAVHPSSHHNTLLFDRADYDRIFALLDRDLIGWVP
CCCCEEEHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
DTGHILRGHKDMADTLRTYRDRIRYIHLKDVDANGTWAMLGKGVCDTHAVIEIAGTAPRF
CCCCEECCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCCCCEEEEEECCCCCC
NGWLVLEEESETAAADPAGAVKTNRQTMRSYGA
CCEEEEECCCCCCCCCCCCCHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]