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The map label for this gene is puuB [H]

Identifier: 13475973

GI number: 13475973

Start: 5922692

End: 5923996

Strand: Reverse

Name: puuB [H]

Synonym: mll7172

Alternate gene names: 13475973

Gene position: 5923996-5922692 (Counterclockwise)

Preceding gene: 13475974

Following gene: 13475968

Centisome position: 84.19

GC content: 66.9

Gene sequence:

>1305_bases
ATGACCGCTGCGATCGGGCAGGAAGCGCATGGCGCTTCGCTCTGGCACGCCGTCAGCCGCAACCGCCGGGAACGGCCTGC
GCTGCAAGGCGGGCTGGATGTCGATCTGGCCATCGTCGGCGGCGGGTTTGCCGGCCTGTCGACCGCGCTGCACGCCGCTG
AAAAAGGCATCTCCGTGGCTGTTCTCGAAGCCGAAATCATCGCCTGGGGCGCGACCGGCCGGAATGCCGGTTTCGTCGTG
CCGAACTTCGCCAAGATGGACCCGGACAGCATCCTTGCGCATCTCGGCCCCGAGCGCGGCGAACGCCTGATCGACTTTGC
CGCCGGCAGTGCAGACCTGGTCTTCGGCCTGATCAAACGGCACGGCATCGACTGCGACGCCGTGCAGAACGGATGGATCC
AACCGGCACATTCGCCTGCCGCCCTCGAAAAGGTCAAATCGCGCGCCGCGCAATGGGCACGGCGTGGCCGGCCTGCGGTC
ACATTGGATCGGCAAGGCGTTGAGGCGCTGACCGGTGCACGCGGCTATGTCGGCGGGTGGATGGACCGCTCGGGTGGCGT
ACTCAATCCGGTCGCCTACGCGAACGGACTGGCCGATGCGGCGCAGAAGGCTGGCGCCAAAATCTTCGAGCGCACGCCGG
TCACTTCGGTCGATCGCGTGGTCGATGGCTGGTCGCTGAAAACGCCATCGGGCTCGGTGCGTGCCGGCAAGGTGCTGATC
GCCACCAACGCCTATGGCGGATCGCTCAATCCGCTGCTGCAACGAAGCTATTTTCCGCTGAAGATTTTCCAGATCGCGAC
AGAGCCTTTGCCGCGCGACGTGCGGACGCGTTTGCTTCCCGGCGGGCAAGGCGTGGGCGACACCAGGCGCAATCTCTTCA
CCTTCCGCTTCGATGCCGACAATCGGCTGATCAGCGGCGGCATGCATATTCTGAGCGCCGGCGCCGACACACGCGTGCCG
CACACGATCTGGCGGCGGCTGGCCAGGCATCTCGACCTGCCGGACCTGCCGCCGCCTGCCTATGGCTGGTCAGGAATGGC
CGCGGTCGAACCGGATTTCCTGCCGCACCTCCTGGATCTTGGACCGGGGCTGATCGCCGGCCGCGCCTGCAACGGGCGCG
GCATCGCCATGACGACCGCGATGGGCAAGGTGCTGGCCGACTGGGCCACCGGAACCGAAGCCCGAGATTTGCCGCTGCCG
TTCGCGCCGCCGGCTCCGGTCCCGTTCCATGCCTTGTTGCGGCACGCGCCCAACATGCTGCTTGGCTGGAGCATGCTGCG
CGACCGGTTGGACGAGACGGCATAG

Upstream 100 bases:

>100_bases
TCCTGCTGATCCGGCCGAACGGGCTGTTCGGCGACAGGCCGCGTCGCCCACGCTTCGGCAAACGAGCAAAAGCGGCAGCG
ATCAAGAGCAGTTCCGGCTC

Downstream 100 bases:

>100_bases
GCCCCGACCCAGCCTGCCGTCGATCAGGTCGTCGTGCGAAGATTTTCTGAAAGAACCGCGGCCGCCACGGCGATGTCCTG
CTCGAGGGAGACCCGGACAG

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTAAIGQEAHGASLWHAVSRNRRERPALQGGLDVDLAIVGGGFAGLSTALHAAEKGISVAVLEAEIIAWGATGRNAGFVV
PNFAKMDPDSILAHLGPERGERLIDFAAGSADLVFGLIKRHGIDCDAVQNGWIQPAHSPAALEKVKSRAAQWARRGRPAV
TLDRQGVEALTGARGYVGGWMDRSGGVLNPVAYANGLADAAQKAGAKIFERTPVTSVDRVVDGWSLKTPSGSVRAGKVLI
ATNAYGGSLNPLLQRSYFPLKIFQIATEPLPRDVRTRLLPGGQGVGDTRRNLFTFRFDADNRLISGGMHILSAGADTRVP
HTIWRRLARHLDLPDLPPPAYGWSGMAAVEPDFLPHLLDLGPGLIAGRACNGRGIAMTTAMGKVLADWATGTEARDLPLP
FAPPAPVPFHALLRHAPNMLLGWSMLRDRLDETA

Sequences:

>Translated_434_residues
MTAAIGQEAHGASLWHAVSRNRRERPALQGGLDVDLAIVGGGFAGLSTALHAAEKGISVAVLEAEIIAWGATGRNAGFVV
PNFAKMDPDSILAHLGPERGERLIDFAAGSADLVFGLIKRHGIDCDAVQNGWIQPAHSPAALEKVKSRAAQWARRGRPAV
TLDRQGVEALTGARGYVGGWMDRSGGVLNPVAYANGLADAAQKAGAKIFERTPVTSVDRVVDGWSLKTPSGSVRAGKVLI
ATNAYGGSLNPLLQRSYFPLKIFQIATEPLPRDVRTRLLPGGQGVGDTRRNLFTFRFDADNRLISGGMHILSAGADTRVP
HTIWRRLARHLDLPDLPPPAYGWSGMAAVEPDFLPHLLDLGPGLIAGRACNGRGIAMTTAMGKVLADWATGTEARDLPLP
FAPPAPVPFHALLRHAPNMLLGWSMLRDRLDETA
>Mature_433_residues
TAAIGQEAHGASLWHAVSRNRRERPALQGGLDVDLAIVGGGFAGLSTALHAAEKGISVAVLEAEIIAWGATGRNAGFVVP
NFAKMDPDSILAHLGPERGERLIDFAAGSADLVFGLIKRHGIDCDAVQNGWIQPAHSPAALEKVKSRAAQWARRGRPAVT
LDRQGVEALTGARGYVGGWMDRSGGVLNPVAYANGLADAAQKAGAKIFERTPVTSVDRVVDGWSLKTPSGSVRAGKVLIA
TNAYGGSLNPLLQRSYFPLKIFQIATEPLPRDVRTRLLPGGQGVGDTRRNLFTFRFDADNRLISGGMHILSAGADTRVPH
TIWRRLARHLDLPDLPPPAYGWSGMAAVEPDFLPHLLDLGPGLIAGRACNGRGIAMTTAMGKVLADWATGTEARDLPLPF
APPAPVPFHALLRHAPNMLLGWSMLRDRLDETA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=429, Percent_Identity=30.0699300699301, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46196; Mature: 46065

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAIGQEAHGASLWHAVSRNRRERPALQGGLDVDLAIVGGGFAGLSTALHAAEKGISVA
CCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
VLEAEIIAWGATGRNAGFVVPNFAKMDPDSILAHLGPERGERLIDFAAGSADLVFGLIKR
EEEEEEEEECCCCCCCCEECCCHHCCCHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHH
HGIDCDAVQNGWIQPAHSPAALEKVKSRAAQWARRGRPAVTLDRQGVEALTGARGYVGGW
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCC
MDRSGGVLNPVAYANGLADAAQKAGAKIFERTPVTSVDRVVDGWSLKTPSGSVRAGKVLI
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHCCCEECCCCCCCCCCEEEE
ATNAYGGSLNPLLQRSYFPLKIFQIATEPLPRDVRTRLLPGGQGVGDTRRNLFTFRFDAD
EECCCCCCCCHHHHCCCCCEEEEEHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCC
NRLISGGMHILSAGADTRVPHTIWRRLARHLDLPDLPPPAYGWSGMAAVEPDFLPHLLDL
CCEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GPGLIAGRACNGRGIAMTTAMGKVLADWATGTEARDLPLPFAPPAPVPFHALLRHAPNML
CCCEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHH
LGWSMLRDRLDETA
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TAAIGQEAHGASLWHAVSRNRRERPALQGGLDVDLAIVGGGFAGLSTALHAAEKGISVA
CCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEE
VLEAEIIAWGATGRNAGFVVPNFAKMDPDSILAHLGPERGERLIDFAAGSADLVFGLIKR
EEEEEEEEECCCCCCCCEECCCHHCCCHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHH
HGIDCDAVQNGWIQPAHSPAALEKVKSRAAQWARRGRPAVTLDRQGVEALTGARGYVGGW
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCC
MDRSGGVLNPVAYANGLADAAQKAGAKIFERTPVTSVDRVVDGWSLKTPSGSVRAGKVLI
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHCCCEECCCCCCCCCCEEEE
ATNAYGGSLNPLLQRSYFPLKIFQIATEPLPRDVRTRLLPGGQGVGDTRRNLFTFRFDAD
EECCCCCCCCHHHHCCCCCEEEEEHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEECCC
NRLISGGMHILSAGADTRVPHTIWRRLARHLDLPDLPPPAYGWSGMAAVEPDFLPHLLDL
CCEECCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
GPGLIAGRACNGRGIAMTTAMGKVLADWATGTEARDLPLPFAPPAPVPFHALLRHAPNML
CCCEEECCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHH
LGWSMLRDRLDETA
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]