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The map label for this gene is mdlA [H]

Identifier: 13475953

GI number: 13475953

Start: 5901625

End: 5902737

Strand: Reverse

Name: mdlA [H]

Synonym: mll7149

Alternate gene names: 13475953

Gene position: 5902737-5901625 (Counterclockwise)

Preceding gene: 13475954

Following gene: 13475952

Centisome position: 83.89

GC content: 62.8

Gene sequence:

>1113_bases
ATGGCTAGAATCGAGAAAGTCGAACTGCGGATGGTGGACCTTGTGCCCAAGGTCAGGCGCACGGATGCCATCCAGAGTTT
CGTCAGCCAGGAAACACCCATCGTCACGATCACCGATTCCGACGGCGCGATCGGCACCGGTTACAGCTACACGATCGGCA
CCGGCGGTTCGTCGGTGATGCGGCTCTTGTCCGACCATCTGGCGCCACGCCTGATCGGCCGCGACCCCGACATGATCGAG
GCGATCTGGCACGATCTCGAATTCGCCACCCATGCGACGACCATCGGTGCGATCACCTCCATCGCCATTGCGGCGATCGA
TACGGCGCTCTGGGATCTGAGGGCGAAGAAGCAGAACCTGCCGCTGTGGAAACTCGCCGGCGGCGCCAAGGACCGCTGCC
CGCTATACACCACCGAGGGCGGCTGGCTGCACATCGAGACCGCAGCACTTGTCGACGACGCACTGGCTGCCAAGGCGAAG
GGATTTCGTGGCTCGAAAGTGAAGATCGGCAGGCCGCACGGGTCGGAGGATCTCGCCCGCCTGTCGGCGGTGCGCAAGGC
TGTGGGCGACGGCTACGAGATCATGACCGACGCCAATCAGGGGTTTTCGGTCGACGAGGCGATCCGGCGCGCGGCGCGGC
TGCGCGAACTCGACCTCGCCTGGATCGAGGAGCCGCTGCCGGCCGACGACATTGACGGCCATATCAGGTTGTCCAACTCG
ACGCCGACGCCAATTGCCATTGGCGAATCGCTCTATTCGATCCGCCATTTTCGCGAATACATGCAGAAAGGCGCCTGCTC
GATCGTGCAGGTCGATGTCGGCCGCATCGGCGGCATCACGCCATGGCTCAAGATCGCCCATGCGGCGGAGGCCTTCGACA
TTCCGGTCTGCCCGCATTTCCTGATGGAGCTGCATGTCAGCTTGACCTGCGCGGTTCAGAACGGCAGGTATGTCGAGTAC
ATTCCGCAACTCGACCAGTTGACCGGCAAGCATATGCGCATCGAGGATGGGCATGCGCTGGCGCCCGACGAACCCGGCAT
CGGCATCGACTGGGATTGGGACGCGGTCAAGGCCATGAGCATCGCCGAATTCACCACGGCGATCACGAAATAG

Upstream 100 bases:

>100_bases
GCGCCCGTTGCGCTCCGGCGAGCGGGTGGCGATGAGCCTCGACCTGTCGCAGGCGCATCTGTTTGCCGCCGAGACCGGAA
AGACATTGCGGAGCTGACCG

Downstream 100 bases:

>100_bases
GAGTCGGATATGCAGCGAATGGGCATGGTGCTGGGGTTGAAGCCGGAAAAGGTCGAGGAGTATGTCCGCCTGCATGCCGC
CGTCTGGCCCGACGTGCTGA

Product: mandelate racemase

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MARIEKVELRMVDLVPKVRRTDAIQSFVSQETPIVTITDSDGAIGTGYSYTIGTGGSSVMRLLSDHLAPRLIGRDPDMIE
AIWHDLEFATHATTIGAITSIAIAAIDTALWDLRAKKQNLPLWKLAGGAKDRCPLYTTEGGWLHIETAALVDDALAAKAK
GFRGSKVKIGRPHGSEDLARLSAVRKAVGDGYEIMTDANQGFSVDEAIRRAARLRELDLAWIEEPLPADDIDGHIRLSNS
TPTPIAIGESLYSIRHFREYMQKGACSIVQVDVGRIGGITPWLKIAHAAEAFDIPVCPHFLMELHVSLTCAVQNGRYVEY
IPQLDQLTGKHMRIEDGHALAPDEPGIGIDWDWDAVKAMSIAEFTTAITK

Sequences:

>Translated_370_residues
MARIEKVELRMVDLVPKVRRTDAIQSFVSQETPIVTITDSDGAIGTGYSYTIGTGGSSVMRLLSDHLAPRLIGRDPDMIE
AIWHDLEFATHATTIGAITSIAIAAIDTALWDLRAKKQNLPLWKLAGGAKDRCPLYTTEGGWLHIETAALVDDALAAKAK
GFRGSKVKIGRPHGSEDLARLSAVRKAVGDGYEIMTDANQGFSVDEAIRRAARLRELDLAWIEEPLPADDIDGHIRLSNS
TPTPIAIGESLYSIRHFREYMQKGACSIVQVDVGRIGGITPWLKIAHAAEAFDIPVCPHFLMELHVSLTCAVQNGRYVEY
IPQLDQLTGKHMRIEDGHALAPDEPGIGIDWDWDAVKAMSIAEFTTAITK
>Mature_369_residues
ARIEKVELRMVDLVPKVRRTDAIQSFVSQETPIVTITDSDGAIGTGYSYTIGTGGSSVMRLLSDHLAPRLIGRDPDMIEA
IWHDLEFATHATTIGAITSIAIAAIDTALWDLRAKKQNLPLWKLAGGAKDRCPLYTTEGGWLHIETAALVDDALAAKAKG
FRGSKVKIGRPHGSEDLARLSAVRKAVGDGYEIMTDANQGFSVDEAIRRAARLRELDLAWIEEPLPADDIDGHIRLSNST
PTPIAIGESLYSIRHFREYMQKGACSIVQVDVGRIGGITPWLKIAHAAEAFDIPVCPHFLMELHVSLTCAVQNGRYVEYI
PQLDQLTGKHMRIEDGHALAPDEPGIGIDWDWDAVKAMSIAEFTTAITK

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=389, Percent_Identity=27.5064267352185, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI42544117, Length=389, Percent_Identity=26.7352185089974, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI186972148, Length=317, Percent_Identity=28.0757097791798, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1787864, Length=389, Percent_Identity=26.7352185089974, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI48994953, Length=275, Percent_Identity=30.1818181818182, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI226510960, Length=346, Percent_Identity=26.3005780346821, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 40483; Mature: 40352

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIEKVELRMVDLVPKVRRTDAIQSFVSQETPIVTITDSDGAIGTGYSYTIGTGGSSVM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCEEEEECCCHHHHH
RLLSDHLAPRLIGRDPDMIEAIWHDLEFATHATTIGAITSIAIAAIDTALWDLRAKKQNL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLWKLAGGAKDRCPLYTTEGGWLHIETAALVDDALAAKAKGFRGSKVKIGRPHGSEDLAR
CEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LSAVRKAVGDGYEIMTDANQGFSVDEAIRRAARLRELDLAWIEEPLPADDIDGHIRLSNS
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
TPTPIAIGESLYSIRHFREYMQKGACSIVQVDVGRIGGITPWLKIAHAAEAFDIPVCPHF
CCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHH
LMELHVSLTCAVQNGRYVEYIPQLDQLTGKHMRIEDGHALAPDEPGIGIDWDWDAVKAMS
HHHHHEEEEEEEECCCEEEECCCHHHHCCCCEEEECCCEECCCCCCCCCCCCHHHHHHHH
IAEFTTAITK
HHHHHHHHCC
>Mature Secondary Structure 
ARIEKVELRMVDLVPKVRRTDAIQSFVSQETPIVTITDSDGAIGTGYSYTIGTGGSSVM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEECCEEEEECCCHHHHH
RLLSDHLAPRLIGRDPDMIEAIWHDLEFATHATTIGAITSIAIAAIDTALWDLRAKKQNL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLWKLAGGAKDRCPLYTTEGGWLHIETAALVDDALAAKAKGFRGSKVKIGRPHGSEDLAR
CEEEECCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
LSAVRKAVGDGYEIMTDANQGFSVDEAIRRAARLRELDLAWIEEPLPADDIDGHIRLSNS
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCC
TPTPIAIGESLYSIRHFREYMQKGACSIVQVDVGRIGGITPWLKIAHAAEAFDIPVCPHF
CCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHH
LMELHVSLTCAVQNGRYVEYIPQLDQLTGKHMRIEDGHALAPDEPGIGIDWDWDAVKAMS
HHHHHEEEEEEEECCCEEEECCCHHHHCCCCEEEECCCEECCCCCCCCCCCCHHHHHHHH
IAEFTTAITK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]