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The map label for this gene is ynfL [H]

Identifier: 13475820

GI number: 13475820

Start: 5750531

End: 5751430

Strand: Direct

Name: ynfL [H]

Synonym: mlr6990

Alternate gene names: 13475820

Gene position: 5750531-5751430 (Clockwise)

Preceding gene: 13475815

Following gene: 13475821

Centisome position: 81.73

GC content: 58.67

Gene sequence:

>900_bases
ATGAAGATATCGGGGTCCGATCTCCATCTGTTTCGCGTCTTTGAAAGCGTGGTCCGCAATGGCGGCATGACCGCAGCTCA
GATGGAGCTGTCGCTTAGCCAACCTACGGTCTCCAACCATCTGACCGCGCTTGAACAGCGGCTCGGCGTGAAGTTGTGCG
AACGGGGGCGTCGCGGGTTTTCGCTGACGGAGGAAGGGCGGCGGGTTTACGAAATAAGTATGGAAATCACCGGGATGCTG
GACGCCGGTTCGGCCCGGCTTTCGCGTGTCAGAAGCGCTTTGGCGGGAAAGGTCGGCGTCGGCATTGTCGACTGCCTGGC
GACCGACCCAAGTTTCCGGCTTGCCGACGCAATTGCCGAGCTTTCCAAAGCTGCACCGGAAATCGAGCTAGATCTCAAGA
TCATGCGCCCCAACGACATAACGAAGGCGGTGGCGGAAAACGAAATAGACATTGGGATAGGTGGCTCCGACATCAAGGTC
GCAAGCGTGCAATACCAGATCCTCTACCACGAGGAGCACGCGGTATTTTGCGGTTCGTCGCATCCCTTGTTTCGCCGAAA
TGACGAGACGATTACGAGTGCGGAATGCTACGGCCATCCATGGGTAGAGAGAGGCTATTGGTATGGTATTCGGCGTCACC
GGTTCGACACCGCAGCCTCGAACCGGGTAACCTTCGATATTGAGGCGCAGCTTCTGATGGTCCTGTCGGGCGCCTACCTC
GGGGTCCTGCCGATACACTTTGCCCGCGTGTTCGAACAGCAGGGGCGTCTTCGCCGACTTCCCCTAGCCGACGAGGCTTA
TTCCGCTGACATCGATGTCGCCACGCGAACAGGCGAGCAGGCCGCCAACGTCGCCTATGTCCGCGACGCGATCATCAGGG
CGCATCGGATGCATTTGTAA

Upstream 100 bases:

>100_bases
CGGTCGCTTCCGGCTTCCGGAATCAAGGCCGTCCTCAATACGGGAAGCCACAGTATGTCTGCCGAATTGCCTTCCCACTC
GTTTGGTGCAATCTGCCTTC

Downstream 100 bases:

>100_bases
AAACCAATATATCGGTTCTCTGAATGATATTCTTGCTACGCCGATGGAGGTCTAGCCTCTGGCACGGCTGGCGAACCGCT
GCGCAAAAATGCGCACCTGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKISGSDLHLFRVFESVVRNGGMTAAQMELSLSQPTVSNHLTALEQRLGVKLCERGRRGFSLTEEGRRVYEISMEITGML
DAGSARLSRVRSALAGKVGVGIVDCLATDPSFRLADAIAELSKAAPEIELDLKIMRPNDITKAVAENEIDIGIGGSDIKV
ASVQYQILYHEEHAVFCGSSHPLFRRNDETITSAECYGHPWVERGYWYGIRRHRFDTAASNRVTFDIEAQLLMVLSGAYL
GVLPIHFARVFEQQGRLRRLPLADEAYSADIDVATRTGEQAANVAYVRDAIIRAHRMHL

Sequences:

>Translated_299_residues
MKISGSDLHLFRVFESVVRNGGMTAAQMELSLSQPTVSNHLTALEQRLGVKLCERGRRGFSLTEEGRRVYEISMEITGML
DAGSARLSRVRSALAGKVGVGIVDCLATDPSFRLADAIAELSKAAPEIELDLKIMRPNDITKAVAENEIDIGIGGSDIKV
ASVQYQILYHEEHAVFCGSSHPLFRRNDETITSAECYGHPWVERGYWYGIRRHRFDTAASNRVTFDIEAQLLMVLSGAYL
GVLPIHFARVFEQQGRLRRLPLADEAYSADIDVATRTGEQAANVAYVRDAIIRAHRMHL
>Mature_299_residues
MKISGSDLHLFRVFESVVRNGGMTAAQMELSLSQPTVSNHLTALEQRLGVKLCERGRRGFSLTEEGRRVYEISMEITGML
DAGSARLSRVRSALAGKVGVGIVDCLATDPSFRLADAIAELSKAAPEIELDLKIMRPNDITKAVAENEIDIGIGGSDIKV
ASVQYQILYHEEHAVFCGSSHPLFRRNDETITSAECYGHPWVERGYWYGIRRHRFDTAASNRVTFDIEAQLLMVLSGAYL
GVLPIHFARVFEQQGRLRRLPLADEAYSADIDVATRTGEQAANVAYVRDAIIRAHRMHL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=272, Percent_Identity=26.8382352941176, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693105, Length=180, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081904, Length=179, Percent_Identity=29.608938547486, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=213, Percent_Identity=26.2910798122066, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33188; Mature: 33188

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISGSDLHLFRVFESVVRNGGMTAAQMELSLSQPTVSNHLTALEQRLGVKLCERGRRGF
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCC
SLTEEGRRVYEISMEITGMLDAGSARLSRVRSALAGKVGVGIVDCLATDPSFRLADAIAE
CCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
LSKAAPEIELDLKIMRPNDITKAVAENEIDIGIGGSDIKVASVQYQILYHEEHAVFCGSS
HHHCCCCEEEEEEEECCCHHHHHHHCCCEEEEECCCCEEEEEEEEEEEEEECCEEEECCC
HPLFRRNDETITSAECYGHPWVERGYWYGIRRHRFDTAASNRVTFDIEAQLLMVLSGAYL
CCCEECCCCCEEEHHHCCCCCHHCCEEECHHHHCCCCCCCCEEEEEHHHHHEEHHHCCHH
GVLPIHFARVFEQQGRLRRLPLADEAYSADIDVATRTGEQAANVAYVRDAIIRAHRMHL
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKISGSDLHLFRVFESVVRNGGMTAAQMELSLSQPTVSNHLTALEQRLGVKLCERGRRGF
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHCCCCCC
SLTEEGRRVYEISMEITGMLDAGSARLSRVRSALAGKVGVGIVDCLATDPSFRLADAIAE
CCCCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHH
LSKAAPEIELDLKIMRPNDITKAVAENEIDIGIGGSDIKVASVQYQILYHEEHAVFCGSS
HHHCCCCEEEEEEEECCCHHHHHHHCCCEEEEECCCCEEEEEEEEEEEEEECCEEEECCC
HPLFRRNDETITSAECYGHPWVERGYWYGIRRHRFDTAASNRVTFDIEAQLLMVLSGAYL
CCCEECCCCCEEEHHHCCCCCHHCCEEECHHHHCCCCCCCCEEEEEHHHHHEEHHHCCHH
GVLPIHFARVFEQQGRLRRLPLADEAYSADIDVATRTGEQAANVAYVRDAIIRAHRMHL
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]