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The map label for this gene is glcF [H]

Identifier: 13475760

GI number: 13475760

Start: 5690126

End: 5691451

Strand: Direct

Name: glcF [H]

Synonym: mlr6920

Alternate gene names: 13475760

Gene position: 5690126-5691451 (Clockwise)

Preceding gene: 13475759

Following gene: 13475762

Centisome position: 80.87

GC content: 64.86

Gene sequence:

>1326_bases
ATGCAGACGAATTTCTCATCCGACCAACTCGCCGACCCGCATGTCGCGGAATCGGAAAAGATCCTGCGCAAATGCGTGCA
TTGCGGCTTCTGCACCGCCACTTGCCCGACCTATGTGACGCTCGGCAACGAGCTCGATTCACCGCGCGGGCGCATCTATC
TGATCAAGGACATGCTGGAGAACGGCCGTCCGGCCGACAAGGAGATCGTCACCCATATCGACCGCTGCCTGTCGTGCCTT
GCCTGCATGACCACATGCCCCTCGGGCGTGAACTACATGCACCTGGTCGATCACGCCCGCGCCCACATCCAGGAGACCTA
TAACAGGCCGCTGCTCGACCGCCTGACGCGCGCCATGCTGGCCTTCGTGCTGCCCTATCCCCGGCGTTTTCGCGCCGCGC
TCAAACTGGCGAAACTCGGCAAGCCCTTCATCGGCCTGTTCGAGAAGGTCCCGGCGCTAAAACCGCTTGGCGCGATGCTC
AAGCTCGCGCCGGCCTCGATCCCGGCAGCCTCGCCGATGGCCTCGCCGGGCGTCCATGCCGGGCAGGGGGCCAAAAAGGG
CCGCGTCGCCATCCTCACCGGCTGCGCGCAATCGGTGCTTGATCCCGCCATCAACGACACGACGATTTCGCTGCTGACGC
GGCTTGGCGTCGAGGTCGTGGTGCCGCCGGGCGAGGGCTGCTGCGGCGCCCTGGTGCACCATATGGGGCGGGAAGAGGCC
GCACTCGCCTCGGCGAGACAGAATGTCGACGCCTGGACACGCTCGATTGAGCAGGGCGGGCTCGATGCCATCATCATCAC
CGCGTCCGGCTGCGGCACGACGATCAAGGACTATGGCTTCATGCTGCGGCTCGACCCGGCCTATGCCGACAAGGCCGCAC
GCGTCTCGGCGCTGGCCAAGGACATCACCGAATATCTGGCAAGCCTCGACTTGCCCGAGCCGGTGCGCAGGCCGGGCACG
GTCGTCGCCTATCACTCCGCCTGTTTGATGCAGCACGGCCAGAAGATCGTCCGCCAGCCGAAGGAGTTGCTGGCCAAAGC
CGGTTTCATCGTTCGGGAGCCGCGCGAGGGCCATCTGTGCTGCGGCTCGGCCGGCACCTACAACATCCTGCAGTCCGAGA
TTTCGGCAAAACTGCGCGACCGCAAGGTGAAAAACATCGAGGCGACGGGCGCCTCGATCGTCGCCACCGGCAACATAGGC
TGCATTACGCAGATCGCGTCAGCCGCCAGCATGCCAGTGGTGCACACCATCAAGCTGCTCGACTGGGCTTATGGCGGACC
GAAGCCGGAGGGTGTCCGAGACGAGAGCCTGATCGCTGCGGAGTAG

Upstream 100 bases:

>100_bases
CCGCAGCCGCCGGCGCTGGCGGCGCTCAGCCAGCGGCTGAAGCAGGAATTCGACCCGAAGAACATCCTCAATCCCGGCCG
CATGGCCTAGGACATCAGCG

Downstream 100 bases:

>100_bases
GGATCGGCGCTTACTCCGCCGCGAGCCTGTCCGCCGTGCCGTAGGTTGCCTCGTAAAGCGCGCGCTGCCGGCTGAGCGCC
ACCATCGTGTTACGCAGCAG

Product: glycolate oxidase subunit, (Fe-S)protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MQTNFSSDQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENGRPADKEIVTHIDRCLSCL
ACMTTCPSGVNYMHLVDHARAHIQETYNRPLLDRLTRAMLAFVLPYPRRFRAALKLAKLGKPFIGLFEKVPALKPLGAML
KLAPASIPAASPMASPGVHAGQGAKKGRVAILTGCAQSVLDPAINDTTISLLTRLGVEVVVPPGEGCCGALVHHMGREEA
ALASARQNVDAWTRSIEQGGLDAIIITASGCGTTIKDYGFMLRLDPAYADKAARVSALAKDITEYLASLDLPEPVRRPGT
VVAYHSACLMQHGQKIVRQPKELLAKAGFIVREPREGHLCCGSAGTYNILQSEISAKLRDRKVKNIEATGASIVATGNIG
CITQIASAASMPVVHTIKLLDWAYGGPKPEGVRDESLIAAE

Sequences:

>Translated_441_residues
MQTNFSSDQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENGRPADKEIVTHIDRCLSCL
ACMTTCPSGVNYMHLVDHARAHIQETYNRPLLDRLTRAMLAFVLPYPRRFRAALKLAKLGKPFIGLFEKVPALKPLGAML
KLAPASIPAASPMASPGVHAGQGAKKGRVAILTGCAQSVLDPAINDTTISLLTRLGVEVVVPPGEGCCGALVHHMGREEA
ALASARQNVDAWTRSIEQGGLDAIIITASGCGTTIKDYGFMLRLDPAYADKAARVSALAKDITEYLASLDLPEPVRRPGT
VVAYHSACLMQHGQKIVRQPKELLAKAGFIVREPREGHLCCGSAGTYNILQSEISAKLRDRKVKNIEATGASIVATGNIG
CITQIASAASMPVVHTIKLLDWAYGGPKPEGVRDESLIAAE
>Mature_441_residues
MQTNFSSDQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLENGRPADKEIVTHIDRCLSCL
ACMTTCPSGVNYMHLVDHARAHIQETYNRPLLDRLTRAMLAFVLPYPRRFRAALKLAKLGKPFIGLFEKVPALKPLGAML
KLAPASIPAASPMASPGVHAGQGAKKGRVAILTGCAQSVLDPAINDTTISLLTRLGVEVVVPPGEGCCGALVHHMGREEA
ALASARQNVDAWTRSIEQGGLDAIIITASGCGTTIKDYGFMLRLDPAYADKAARVSALAKDITEYLASLDLPEPVRRPGT
VVAYHSACLMQHGQKIVRQPKELLAKAGFIVREPREGHLCCGSAGTYNILQSEISAKLRDRKVKNIEATGASIVATGNIG
CITQIASAASMPVVHTIKLLDWAYGGPKPEGVRDESLIAAE

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=423, Percent_Identity=43.2624113475177, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1788576, Length=421, Percent_Identity=24.7030878859857, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786497, Length=223, Percent_Identity=24.6636771300448, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 47337; Mature: 47337

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNFSSDQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCEEEEEHHHHH
NGRPADKEIVTHIDRCLSCLACMTTCPSGVNYMHLVDHARAHIQETYNRPLLDRLTRAML
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AFVLPYPRRFRAALKLAKLGKPFIGLFEKVPALKPLGAMLKLAPASIPAASPMASPGVHA
HHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
GQGAKKGRVAILTGCAQSVLDPAINDTTISLLTRLGVEVVVPPGEGCCGALVHHMGREEA
CCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHH
ALASARQNVDAWTRSIEQGGLDAIIITASGCGTTIKDYGFMLRLDPAYADKAARVSALAK
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCEEEEECHHHHHHHHHHHHHHH
DITEYLASLDLPEPVRRPGTVVAYHSACLMQHGQKIVRQPKELLAKAGFIVREPREGHLC
HHHHHHHHCCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEE
CGSAGTYNILQSEISAKLRDRKVKNIEATGASIVATGNIGCITQIASAASMPVVHTIKLL
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHH
DWAYGGPKPEGVRDESLIAAE
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQTNFSSDQLADPHVAESEKILRKCVHCGFCTATCPTYVTLGNELDSPRGRIYLIKDMLE
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCEEEEEHHHHH
NGRPADKEIVTHIDRCLSCLACMTTCPSGVNYMHLVDHARAHIQETYNRPLLDRLTRAML
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AFVLPYPRRFRAALKLAKLGKPFIGLFEKVPALKPLGAMLKLAPASIPAASPMASPGVHA
HHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
GQGAKKGRVAILTGCAQSVLDPAINDTTISLLTRLGVEVVVPPGEGCCGALVHHMGREEA
CCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHH
ALASARQNVDAWTRSIEQGGLDAIIITASGCGTTIKDYGFMLRLDPAYADKAARVSALAK
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCEEEEECHHHHHHHHHHHHHHH
DITEYLASLDLPEPVRRPGTVVAYHSACLMQHGQKIVRQPKELLAKAGFIVREPREGHLC
HHHHHHHHCCCCCHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEE
CGSAGTYNILQSEISAKLRDRKVKNIEATGASIVATGNIGCITQIASAASMPVVHTIKLL
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHH
DWAYGGPKPEGVRDESLIAAE
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]