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The map label for this gene is hemK [H]

Identifier: 13475623

GI number: 13475623

Start: 5533265

End: 5533957

Strand: Direct

Name: hemK [H]

Synonym: mlr6740

Alternate gene names: 13475623

Gene position: 5533265-5533957 (Clockwise)

Preceding gene: 13475622

Following gene: 13475629

Centisome position: 78.64

GC content: 64.5

Gene sequence:

>693_bases
ATGAGCAAAGAGTTCAGTGTTGCCGACGCCTATGGCTCGGGCCGCGCCATCTTCATGGGACTTGAGCTGCTCGTCGCGCC
TGGTGCGCTCGTGCCCCGGCCAGAGACGGAATTGCTGGGCACGACGGCCCTTGGTGTCTTGCATCAAATGAACCTGCCGG
CCCCGCGCATCGTCGACATGTGCTGCGGCACTGGCAATCTGGCATGCGCCATCGCCCACCACATTCCCGCGGCACGCGTC
TGGGCGAGCGACCTTACCGATGGATGCGTCGACGTGGCACGCCGTAACGCCGCCCATCACGGCATGGCTGACCGTGTTTC
GGTGCTTCAGGGCGACTTGTTCGACGCTCTCTCGGGCCTGGGGCTTGACCGGACGATCGACGTGATCGTCTGCAATCCGC
CCTACATTTCAGAAAAGCGGCTCGAGGGCGACCGCGCACATCTGGTCGAGCTCGAGCCGCGTGAGGCGTTCGCGGCCGGC
CCTTACGGCCTGAGCATCCATATGCGGGTGGTGAAAGACGCGCCGCTATACTTGCGGCCTGGCGGCATCCTGCTCTTCGA
GGTCGGCCTTGGCCAAGATCGGCAGGTGATGGGACTGCTGGAGCGCAGCAAGCAATACGAGAACATCCGCGCTGTGTCGA
ACGAAGCGGGCGAGGGCCGGGTGGTTCTGGGACAAGCCAGGGCGCAGGCATGA

Upstream 100 bases:

>100_bases
TGAAAGACTGACCGGCTGGCGACTTGCCCGGATTCTGCAACGACAGGTTTGACCGGCGTGCGAATGCCGAACACCTATAC
AGGGAAGATCAGGGGTTACC

Downstream 100 bases:

>100_bases
GCGTGCGGAAAGCCAGGGCAATCGCCGGAACCGATCTGGGCTTTATGCCGACGATCAGTTCATTTTGCGGATGATGTATT
GCTCGATCCCTTGAATGGAA

Product: methyl transferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MSKEFSVADAYGSGRAIFMGLELLVAPGALVPRPETELLGTTALGVLHQMNLPAPRIVDMCCGTGNLACAIAHHIPAARV
WASDLTDGCVDVARRNAAHHGMADRVSVLQGDLFDALSGLGLDRTIDVIVCNPPYISEKRLEGDRAHLVELEPREAFAAG
PYGLSIHMRVVKDAPLYLRPGGILLFEVGLGQDRQVMGLLERSKQYENIRAVSNEAGEGRVVLGQARAQA

Sequences:

>Translated_230_residues
MSKEFSVADAYGSGRAIFMGLELLVAPGALVPRPETELLGTTALGVLHQMNLPAPRIVDMCCGTGNLACAIAHHIPAARV
WASDLTDGCVDVARRNAAHHGMADRVSVLQGDLFDALSGLGLDRTIDVIVCNPPYISEKRLEGDRAHLVELEPREAFAAG
PYGLSIHMRVVKDAPLYLRPGGILLFEVGLGQDRQVMGLLERSKQYENIRAVSNEAGEGRVVLGQARAQA
>Mature_229_residues
SKEFSVADAYGSGRAIFMGLELLVAPGALVPRPETELLGTTALGVLHQMNLPAPRIVDMCCGTGNLACAIAHHIPAARVW
ASDLTDGCVDVARRNAAHHGMADRVSVLQGDLFDALSGLGLDRTIDVIVCNPPYISEKRLEGDRAHLVELEPREAFAAGP
YGLSIHMRVVKDAPLYLRPGGILLFEVGLGQDRQVMGLLERSKQYENIRAVSNEAGEGRVVLGQARAQA

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=212, Percent_Identity=28.3018867924528, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787463, Length=219, Percent_Identity=32.8767123287671, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082085, Length=181, Percent_Identity=34.2541436464088, Blast_Score=90, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24582226, Length=182, Percent_Identity=28.021978021978, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24645; Mature: 24514

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFSVADAYGSGRAIFMGLELLVAPGALVPRPETELLGTTALGVLHQMNLPAPRIVDM
CCCCCCEEECCCCCCEEEEEHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
CCGTGNLACAIAHHIPAARVWASDLTDGCVDVARRNAAHHGMADRVSVLQGDLFDALSGL
HCCCCCCEEEHHHCCCHHHHHHHHCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHCC
GLDRTIDVIVCNPPYISEKRLEGDRAHLVELEPREAFAAGPYGLSIHMRVVKDAPLYLRP
CCCCEEEEEEECCCCCCHHHCCCCCEEEEEECCHHHHCCCCCCEEEEEEEEECCCEEECC
GGILLFEVGLGQDRQVMGLLERSKQYENIRAVSNEAGEGRVVLGQARAQA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
SKEFSVADAYGSGRAIFMGLELLVAPGALVPRPETELLGTTALGVLHQMNLPAPRIVDM
CCCCCEEECCCCCCEEEEEHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
CCGTGNLACAIAHHIPAARVWASDLTDGCVDVARRNAAHHGMADRVSVLQGDLFDALSGL
HCCCCCCEEEHHHCCCHHHHHHHHCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHHCC
GLDRTIDVIVCNPPYISEKRLEGDRAHLVELEPREAFAAGPYGLSIHMRVVKDAPLYLRP
CCCCEEEEEEECCCCCCHHHCCCCCEEEEEECCHHHHCCCCCCEEEEEEEEECCCEEECC
GGILLFEVGLGQDRQVMGLLERSKQYENIRAVSNEAGEGRVVLGQARAQA
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]