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The map label for this gene is icaA [H]

Identifier: 13475588

GI number: 13475588

Start: 5491048

End: 5492475

Strand: Direct

Name: icaA [H]

Synonym: mlr6694

Alternate gene names: 13475588

Gene position: 5491048-5492475 (Clockwise)

Preceding gene: 13475587

Following gene: 13474961

Centisome position: 78.04

GC content: 63.24

Gene sequence:

>1428_bases
ATGATGTCCGACTGGAGCCACGAGATCGTTCTGGCCGCTTCCATCCTGTCCTGGTTCATCATCGGGGCGGGGCTGGCGCA
GACGACGATCTATCTGTTGCAACTCATTATTGCCGCCTACGCGCTTTCCAAGCGCCCGCCGGTCGCGCGCTCGGCACTGC
TGTGGCACCGCTATGGCGATGTCGCGCCGCCGATCGCGCTCCTCGTACCCGCCTATAATGAGGAATTGAACGTGGTCGAG
AGCGTCCATTCGATGCTGGCGCTCGAGTATCCGAATTTCGAGGTGATCGTCATCAACGACGGCTCAAAGGACCAGACGCT
GCAGCGCCTGATCGGGGCCTTCAAGCTGGTGAAATTCCGCCGGCCTTATGAGGAGGCGCTGGCGCACAAGCCGATCCGCG
GCCTCTATTCCTCGCCAATGACCGAGCGCCTGTTCGTGGTCGACAAGGAAAACGGCGGCAAGGCCGATGCCCAGAATGCC
GGCATCAATGTCTGCCGCGCGCCGCTTTTCTGCGTGATCGACGGCGATTCCATCCTCGAGCCCGATGCGCTGATGCGCGC
CGCCCAGCCCTTCATCGACGACCCCGAGCGCACCATCGCCGTCGGCGGCACGATCCGCATCGCCAACGGATCGAGGATCG
AAGCCGGCAGGGTGCGAGAGATCCATCTGCCCAACCGCTTGCTGCCGTTGCTCCAGGTCGTCGAATATCTAAGGGCCTTT
CTCATGGCCCGGCTGGCCTGGAGCCACATCAACACGTTGATGCTGGTGTCGGGCGCCTTCGGCATGTTCCGCCGCACGGA
GGTGGTGGCGGTGGGCGGCTTCACCATGGGCTCGATGGGCGAGGACCTCGACCTCGTCATCAAGCTGCACCGCCACATGA
GAGATGCCGGTCGCAAATACCGCATCCAGTTCATTCCGGAGCCGGTGTGCTGGACCGAGGCGCCGGAAACGCTGAGCGTG
CTCGCACGGCAGCGCACGCGCTGGCAGCGCGGCGCGCTCGAGTGCTTCTTCCGCTACCGCGCCATGCTCTTCAACCCGCG
CTACGGCCGCGTCGGATTCCTGGGCTTCGGCCACATATTGATTGTCGACGTGCTAGGGCCGGTAGCGGAGGTGTTGGGCT
ACATCCTGATCCCGGCATTCTGGCTGCTCGGCATCCTGTCGACCGAGTATCTGCTGGCCTTCACCTCGCTCATCTTCACC
TATGGCGTTTGCGTCAGCGTGTGCTCACTGGTCCTCGAAGAGGCTGAGCTGCAGCGTTTTCCGCGCGCCAGGGACCTGGC
GGTGCTGACGGCGGTGGCGGTGATCGAGAATTTCGGCTACCGCCAGCTCAACAATTTCTGGCGCGTGAAGGGCTGGTGGC
AATATCTGCGGGCCGACCAGAGCTGGGGCGAAATGACCAGGACTGGCCTGTCCGGGGCGAAGAAATAA

Upstream 100 bases:

>100_bases
TTGGCCAGCCGGGAGAACGGCTGCTGCGCGACATCGCTGCCAGCGAGGTCTCGCGCAGCCAGCGCACCGCCTCGCTCATC
CTTGCCGAGGGGCCGGCCGC

Downstream 100 bases:

>100_bases
CGCCCGCTTCATTTCAGCGTTTGCTCAATCTCCGCGATGAGCGCATCGAGCGCGGCTTGGACGGCTCCTTCATTCGGCTG
TTCGATTAGCAGCGTTTCCA

Product: hypothetical protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MMSDWSHEIVLAASILSWFIIGAGLAQTTIYLLQLIIAAYALSKRPPVARSALLWHRYGDVAPPIALLVPAYNEELNVVE
SVHSMLALEYPNFEVIVINDGSKDQTLQRLIGAFKLVKFRRPYEEALAHKPIRGLYSSPMTERLFVVDKENGGKADAQNA
GINVCRAPLFCVIDGDSILEPDALMRAAQPFIDDPERTIAVGGTIRIANGSRIEAGRVREIHLPNRLLPLLQVVEYLRAF
LMARLAWSHINTLMLVSGAFGMFRRTEVVAVGGFTMGSMGEDLDLVIKLHRHMRDAGRKYRIQFIPEPVCWTEAPETLSV
LARQRTRWQRGALECFFRYRAMLFNPRYGRVGFLGFGHILIVDVLGPVAEVLGYILIPAFWLLGILSTEYLLAFTSLIFT
YGVCVSVCSLVLEEAELQRFPRARDLAVLTAVAVIENFGYRQLNNFWRVKGWWQYLRADQSWGEMTRTGLSGAKK

Sequences:

>Translated_475_residues
MMSDWSHEIVLAASILSWFIIGAGLAQTTIYLLQLIIAAYALSKRPPVARSALLWHRYGDVAPPIALLVPAYNEELNVVE
SVHSMLALEYPNFEVIVINDGSKDQTLQRLIGAFKLVKFRRPYEEALAHKPIRGLYSSPMTERLFVVDKENGGKADAQNA
GINVCRAPLFCVIDGDSILEPDALMRAAQPFIDDPERTIAVGGTIRIANGSRIEAGRVREIHLPNRLLPLLQVVEYLRAF
LMARLAWSHINTLMLVSGAFGMFRRTEVVAVGGFTMGSMGEDLDLVIKLHRHMRDAGRKYRIQFIPEPVCWTEAPETLSV
LARQRTRWQRGALECFFRYRAMLFNPRYGRVGFLGFGHILIVDVLGPVAEVLGYILIPAFWLLGILSTEYLLAFTSLIFT
YGVCVSVCSLVLEEAELQRFPRARDLAVLTAVAVIENFGYRQLNNFWRVKGWWQYLRADQSWGEMTRTGLSGAKK
>Mature_475_residues
MMSDWSHEIVLAASILSWFIIGAGLAQTTIYLLQLIIAAYALSKRPPVARSALLWHRYGDVAPPIALLVPAYNEELNVVE
SVHSMLALEYPNFEVIVINDGSKDQTLQRLIGAFKLVKFRRPYEEALAHKPIRGLYSSPMTERLFVVDKENGGKADAQNA
GINVCRAPLFCVIDGDSILEPDALMRAAQPFIDDPERTIAVGGTIRIANGSRIEAGRVREIHLPNRLLPLLQVVEYLRAF
LMARLAWSHINTLMLVSGAFGMFRRTEVVAVGGFTMGSMGEDLDLVIKLHRHMRDAGRKYRIQFIPEPVCWTEAPETLSV
LARQRTRWQRGALECFFRYRAMLFNPRYGRVGFLGFGHILIVDVLGPVAEVLGYILIPAFWLLGILSTEYLLAFTSLIFT
YGVCVSVCSLVLEEAELQRFPRARDLAVLTAVAVIENFGYRQLNNFWRVKGWWQYLRADQSWGEMTRTGLSGAKK

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=352, Percent_Identity=28.6931818181818, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 53504; Mature: 53504

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSDWSHEIVLAASILSWFIIGAGLAQTTIYLLQLIIAAYALSKRPPVARSALLWHRYGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
VAPPIALLVPAYNEELNVVESVHSMLALEYPNFEVIVINDGSKDQTLQRLIGAFKLVKFR
CCCCCEEEECCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
RPYEEALAHKPIRGLYSSPMTERLFVVDKENGGKADAQNAGINVCRAPLFCVIDGDSILE
CCHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCEEEEECCCCCCC
PDALMRAAQPFIDDPERTIAVGGTIRIANGSRIEAGRVREIHLPNRLLPLLQVVEYLRAF
HHHHHHHHCCCCCCCCCEEEECCEEEECCCCEECCCCEEEECCCHHHHHHHHHHHHHHHH
LMARLAWSHINTLMLVSGAFGMFRRTEVVAVGGFTMGSMGEDLDLVIKLHRHMRDAGRKY
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEE
RIQFIPEPVCWTEAPETLSVLARQRTRWQRGALECFFRYRAMLFNPRYGRVGFLGFGHIL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
IVDVLGPVAEVLGYILIPAFWLLGILSTEYLLAFTSLIFTYGVCVSVCSLVLEEAELQRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRARDLAVLTAVAVIENFGYRQLNNFWRVKGWWQYLRADQSWGEMTRTGLSGAKK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMSDWSHEIVLAASILSWFIIGAGLAQTTIYLLQLIIAAYALSKRPPVARSALLWHRYGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
VAPPIALLVPAYNEELNVVESVHSMLALEYPNFEVIVINDGSKDQTLQRLIGAFKLVKFR
CCCCCEEEECCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHC
RPYEEALAHKPIRGLYSSPMTERLFVVDKENGGKADAQNAGINVCRAPLFCVIDGDSILE
CCHHHHHHCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCEEEEECCCCCCC
PDALMRAAQPFIDDPERTIAVGGTIRIANGSRIEAGRVREIHLPNRLLPLLQVVEYLRAF
HHHHHHHHCCCCCCCCCEEEECCEEEECCCCEECCCCEEEECCCHHHHHHHHHHHHHHHH
LMARLAWSHINTLMLVSGAFGMFRRTEVVAVGGFTMGSMGEDLDLVIKLHRHMRDAGRKY
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEE
RIQFIPEPVCWTEAPETLSVLARQRTRWQRGALECFFRYRAMLFNPRYGRVGFLGFGHIL
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHH
IVDVLGPVAEVLGYILIPAFWLLGILSTEYLLAFTSLIFTYGVCVSVCSLVLEEAELQRF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PRARDLAVLTAVAVIENFGYRQLNNFWRVKGWWQYLRADQSWGEMTRTGLSGAKK
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]