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The map label for this gene is bioF [H]

Identifier: 13475562

GI number: 13475562

Start: 5459036

End: 5459860

Strand: Reverse

Name: bioF [H]

Synonym: mll6664

Alternate gene names: 13475562

Gene position: 5459860-5459036 (Counterclockwise)

Preceding gene: 13475563

Following gene: 13475561

Centisome position: 77.6

GC content: 65.82

Gene sequence:

>825_bases
ATGCAGGCCTATACCGACCTGGGCGAGCGGCTTTGCACGTGGTTCGGCGTCGAGCACGTCGTTTCCATGATATCGGGCTA
CACTGGCCCGATGACGCTGCTGCAGGGGCTGCGCGACGATTTCGATGTGGTGTTCGTCGATGCCGCCACGCACTACAGCG
CCCGCGACGCCATCCCCACGCTCGGCAAGCCAGCCCACAGCTTCGGCCATCTCGATGCCGGCAGCCTGGCTGAAGCGTTG
TCGCGTGATGTCGGAACGGGGCAGCGTCCCGTTGTGGTGGTTGACGGCGTCTTTCCCTCGACAGGCGCATTGGCGCCGCT
CGCGGATTGCCCCAAAGCGATGGCGCCGTATAACGGCGCGCTTTTATGCATCGACGATTCCCACGGCGCCGGCGCGATCG
GCGAGAGTGGGCGCGGGTCGCTTGAACATGCCTGCCTCGAGACTGAAGGAAACTATTTTGCGGGCACGCTGAGCAAGGCT
TTCGGCGCGCTCGGCGGCGTCATTCCGGGCGACGCGGCACTTGCCGAGAAGGTAGGCCGCAACGCCATGATCATGCGCGG
CGCGTCGCTGCCGCCGCCGAGCGCGGCGGCAGCGGCTGCGCATCCTGCAGACGACGCCGCGAATGCGTTGCGACCTCGTC
CGCAATGTGCGCCGCATGAGAAGCGGTTTGCGAGAGCTGGGCTTCGATGTCGACGACACGCCGATCCCTATCGTTTCCAT
CAGGGGCGACATCGACTTCGACGCGCTGCAAGCCCGGCTCGACGAACGCGACATCGTCGTCAAGGTGACGCGCGGGATAT
TCCGACGCCCCCGACGTGCCGATGA

Upstream 100 bases:

>100_bases
TCGATCATTTCTGCAGCACCAGCTACTTCTGTTTGCATGGCCACCCCGAGGTCATCGAGGCCGCCTGCACCGCCACGCGG
CAGTTCGGCATGGGTCCCGG

Downstream 100 bases:

>100_bases
TGCGGCTGGCGATGTTTTCCGAGCATTCTCCCGAGCAGATCAACCGGCTGCTGTCGTCGATGGCCGAGCTACTTTGAAAC
AGGAAGACAGATCATGAAGG

Product: 2-amino-3-ketobutyrate CoA ligase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQAYTDLGERLCTWFGVEHVVSMISGYTGPMTLLQGLRDDFDVVFVDAATHYSARDAIPTLGKPAHSFGHLDAGSLAEAL
SRDVGTGQRPVVVVDGVFPSTGALAPLADCPKAMAPYNGALLCIDDSHGAGAIGESGRGSLEHACLETEGNYFAGTLSKA
FGALGGVIPGDAALAEKVGRNAMIMRGASLPPPSAAAAAAHPADDAANALRPRPQCAPHEKRFARAGLRCRRHADPYRFH
QGRHRLRRAASPARRTRHRRQGDARDIPTPPTCR

Sequences:

>Translated_274_residues
MQAYTDLGERLCTWFGVEHVVSMISGYTGPMTLLQGLRDDFDVVFVDAATHYSARDAIPTLGKPAHSFGHLDAGSLAEAL
SRDVGTGQRPVVVVDGVFPSTGALAPLADCPKAMAPYNGALLCIDDSHGAGAIGESGRGSLEHACLETEGNYFAGTLSKA
FGALGGVIPGDAALAEKVGRNAMIMRGASLPPPSAAAAAAHPADDAANALRPRPQCAPHEKRFARAGLRCRRHADPYRFH
QGRHRLRRAASPARRTRHRRQGDARDIPTPPTCR
>Mature_274_residues
MQAYTDLGERLCTWFGVEHVVSMISGYTGPMTLLQGLRDDFDVVFVDAATHYSARDAIPTLGKPAHSFGHLDAGSLAEAL
SRDVGTGQRPVVVVDGVFPSTGALAPLADCPKAMAPYNGALLCIDDSHGAGAIGESGRGSLEHACLETEGNYFAGTLSKA
FGALGGVIPGDAALAEKVGRNAMIMRGASLPPPSAAAAAAHPADDAANALRPRPQCAPHEKRFARAGLRCRRHADPYRFH
QGRHRLRRAASPARRTRHRRQGDARDIPTPPTCR

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1786993, Length=227, Percent_Identity=30.8370044052863, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790046, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320267, Length=210, Percent_Identity=27.1428571428571, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323954, Length=171, Percent_Identity=26.9005847953216, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24662918, Length=224, Percent_Identity=25.8928571428571, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137420, Length=150, Percent_Identity=32.6666666666667, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 29004; Mature: 29004

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYTDLGERLCTWFGVEHVVSMISGYTGPMTLLQGLRDDFDVVFVDAATHYSARDAIPT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHCCCC
LGKPAHSFGHLDAGSLAEALSRDVGTGQRPVVVVDGVFPSTGALAPLADCPKAMAPYNGA
CCCCCHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCHHHCCCCCE
LLCIDDSHGAGAIGESGRGSLEHACLETEGNYFAGTLSKAFGALGGVIPGDAALAEKVGR
EEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCC
NAMIMRGASLPPPSAAAAAAHPADDAANALRPRPQCAPHEKRFARAGLRCRRHADPYRFH
CEEEEECCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
QGRHRLRRAASPARRTRHRRQGDARDIPTPPTCR
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQAYTDLGERLCTWFGVEHVVSMISGYTGPMTLLQGLRDDFDVVFVDAATHYSARDAIPT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEECCCCCHHHHCCCC
LGKPAHSFGHLDAGSLAEALSRDVGTGQRPVVVVDGVFPSTGALAPLADCPKAMAPYNGA
CCCCCHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHCCHHHCCCCCE
LLCIDDSHGAGAIGESGRGSLEHACLETEGNYFAGTLSKAFGALGGVIPGDAALAEKVGR
EEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCC
NAMIMRGASLPPPSAAAAAAHPADDAANALRPRPQCAPHEKRFARAGLRCRRHADPYRFH
CEEEEECCCCCCCCHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
QGRHRLRRAASPARRTRHRRQGDARDIPTPPTCR
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA