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The map label for this gene is fixK [H]

Identifier: 13475493

GI number: 13475493

Start: 5388497

End: 5389144

Strand: Reverse

Name: fixK [H]

Synonym: mll6578

Alternate gene names: 13475493

Gene position: 5389144-5388497 (Counterclockwise)

Preceding gene: 13475497

Following gene: 13475492

Centisome position: 76.59

GC content: 60.96

Gene sequence:

>648_bases
ATGCACGCCTACACCGCCTCCAGAATTTCGTTGCCGTCGCATTCTGCTCAGCCAAGCCTGCCAGTGGCCTTCGACTCGGA
AACGCAACAGCCGGTCAGCTTCTTTCCCGCCGGTTCGGAGATTTATGCCCAAGGCGAGAAGGCCGGCGCTTTCTACCAGG
TCGAATTCGGCGCTGTGCGCATCTACCGCCTGCTCGCAGACGGCCGTCGGCAGATCAGCGCCTTCCACCTAGCCGGAGAG
ACTTTCGGCTTCGAAGCCGATACGACGCATCATTTCTTCGCCGAAGCGATCAATGCCACCGGCGTACGCGCCTTTCGCTT
CAGTGCAGGGGCGGACATGTCCCATCATTTGCTTCCCCTGGCGCTCAAGGGTCTGACTAGAGCGCAGGAACACCTCCTGG
TTCTCGGCCGCCAGAACGCCATCGAACGCGTAGCCGCTTTCCTGGCCGACATGGCAGAGCGGCAGGGCGGGTTGCGGCAG
GTTGAGTTGCCAATGTCGCGTATGGACATCGGCGACTATCTCGGCCTCACCATCGAGACCGTGTCGCGGGTCTTCACCCG
GCTGAAGGACAAGGGCGTCATCCGCCTGATCAACCTGCGCAGCATCGAAATCGTCAAGCAGGAGGTGTTGCAAACCTTGG
GCGAATGA

Upstream 100 bases:

>100_bases
AATCCGCACGACTAGGTAGTTTCCCGTAGGGATAAAACCGAATTTCGCGGCACCCAGATTCGCGCGATTTCTGTGTCACC
GAACAACCGGGAAAACAGCC

Downstream 100 bases:

>100_bases
GGGCGCCTGCCTGCTCAAGAGAGACGACAATGACGCCGCAGAACCGACCCCAACTCAAGGACCAGATGACGATGGACAAC
ATACTTACCACCCATACCGG

Product: transcriptional regulator FixK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MHAYTASRISLPSHSAQPSLPVAFDSETQQPVSFFPAGSEIYAQGEKAGAFYQVEFGAVRIYRLLADGRRQISAFHLAGE
TFGFEADTTHHFFAEAINATGVRAFRFSAGADMSHHLLPLALKGLTRAQEHLLVLGRQNAIERVAAFLADMAERQGGLRQ
VELPMSRMDIGDYLGLTIETVSRVFTRLKDKGVIRLINLRSIEIVKQEVLQTLGE

Sequences:

>Translated_215_residues
MHAYTASRISLPSHSAQPSLPVAFDSETQQPVSFFPAGSEIYAQGEKAGAFYQVEFGAVRIYRLLADGRRQISAFHLAGE
TFGFEADTTHHFFAEAINATGVRAFRFSAGADMSHHLLPLALKGLTRAQEHLLVLGRQNAIERVAAFLADMAERQGGLRQ
VELPMSRMDIGDYLGLTIETVSRVFTRLKDKGVIRLINLRSIEIVKQEVLQTLGE
>Mature_215_residues
MHAYTASRISLPSHSAQPSLPVAFDSETQQPVSFFPAGSEIYAQGEKAGAFYQVEFGAVRIYRLLADGRRQISAFHLAGE
TFGFEADTTHHFFAEAINATGVRAFRFSAGADMSHHLLPLALKGLTRAQEHLLVLGRQNAIERVAAFLADMAERQGGLRQ
VELPMSRMDIGDYLGLTIETVSRVFTRLKDKGVIRLINLRSIEIVKQEVLQTLGE

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=181, Percent_Identity=32.5966850828729, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 23759; Mature: 23759

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYTASRISLPSHSAQPSLPVAFDSETQQPVSFFPAGSEIYAQGEKAGAFYQVEFGAVR
CCCCCCCEEECCCCCCCCCCCEEECCCCCCCCEECCCCCCEEECCCCCCCEEEEEHHHHH
IYRLLADGRRQISAFHLAGETFGFEADTTHHFFAEAINATGVRAFRFSAGADMSHHLLPL
HHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
ALKGLTRAQEHLLVLGRQNAIERVAAFLADMAERQGGLRQVELPMSRMDIGDYLGLTIET
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCHHHHHHHHHHH
VSRVFTRLKDKGVIRLINLRSIEIVKQEVLQTLGE
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHAYTASRISLPSHSAQPSLPVAFDSETQQPVSFFPAGSEIYAQGEKAGAFYQVEFGAVR
CCCCCCCEEECCCCCCCCCCCEEECCCCCCCCEECCCCCCEEECCCCCCCEEEEEHHHHH
IYRLLADGRRQISAFHLAGETFGFEADTTHHFFAEAINATGVRAFRFSAGADMSHHLLPL
HHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
ALKGLTRAQEHLLVLGRQNAIERVAAFLADMAERQGGLRQVELPMSRMDIGDYLGLTIET
HHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCHHHHHHHHHHH
VSRVFTRLKDKGVIRLINLRSIEIVKQEVLQTLGE
HHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]