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The map label for this gene is potH [H]

Identifier: 13475458

GI number: 13475458

Start: 5339857

End: 5340825

Strand: Direct

Name: potH [H]

Synonym: mlr6536

Alternate gene names: 13475458

Gene position: 5339857-5340825 (Clockwise)

Preceding gene: 13475457

Following gene: 13475459

Centisome position: 75.89

GC content: 61.2

Gene sequence:

>969_bases
ATGTCCAACATCGCCGTCGCCGATGCCGCCGCGCCACCGACCGCCGCCAAGCCGTTGCTGACGCGGCTGGGTGCCGGCTT
CGTCAACCGGCTCGTCATCATCGTCCCCTACCTCTGGCTGCTGTTCTTCTTCCTCATCCCCTTCGTCATCGTCTTCAAGA
TTTCATTGTCGCAGACGGCGATCGCCATGCCGCCCTACACGCCGGTGCTCGACTTCAGCGACGGCATCTCCGGTTTCTTC
GCCGGGTTCCGCGACCTCAACTTCGACAATTATGTCTGGCTGACGCAGGACGCGCTCTATTTCAAGGCCTATGTGACGAG
CGTCATCATTGCCGCGATCTCGACCGTGCTGACGCTGATCGTCGGCTACCCCATCGCCTATGGCATGTCACGCGCGCCGG
CTACTATCCGCCCGACCTTGCTGATGCTGGTCATTCTGCCGTTCTGGACGTCGTTCCTGATCCGCGTCTATGCCTGGATC
GGCATTCTGAAGCCCGAGGGCCTGCTCAACCAGCTGCTCTTGTCGCTGCATATCATCAACCAGCCGCTGGTCATCCTCAA
CACCTATACGGCGATCTTCATCGGCATCGTCTATTCCTACCTGCCGTTCATGGTGCTGCCGCTCTATTCGTCACTGGAAA
AGATGGATTACTCGCTGATCGAGGCGGCCAAGGATCTGGGCTGCCCGCCGACCGCGGCTTTCTGGAAAATCACCTTCCCG
CTGTCGCTGCCGGGCGTCATTGCCGGCTGCCTGCTGGTGTTCATCCCGGCCGTCGGCGAGTTCGTCATTCCCGACCTGCT
CGGCGGCTCGCAGACGCTGATGATCGGCAAGACGCTGTGGAACGAGTTCTTCGCCAACCGCGACTGGCCGGTCTCGTCGG
CCGTCGCCGTCATCCTGCTTTTGCTGCTCACCGTGCCGATCATGCTTTTCCAGCAGGCACAGGCGCGTGCCCAGGAGCAG
GGCAAATGA

Upstream 100 bases:

>100_bases
CGCGGCGCGCGTGACCGAGGATCCACTGACCTGGAACGACCGCGCCTGGGTGTCCTTCCGGCCTGATGCCGGCGTCGTGC
TGGCGCGGTAGGGGGCTGAT

Downstream 100 bases:

>100_bases
ACACCACCTGGAGCCGCTTCAACGTCACCTCGATCGTGCTTGGCTTTGCCTTCCTGTACCTGCCGATCGTGCTGCTCATC
GTCTTCTCCTTCAACGAATC

Product: ABC transporter, polyamine transport protein, permease protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSNIAVADAAAPPTAAKPLLTRLGAGFVNRLVIIVPYLWLLFFFLIPFVIVFKISLSQTAIAMPPYTPVLDFSDGISGFF
AGFRDLNFDNYVWLTQDALYFKAYVTSVIIAAISTVLTLIVGYPIAYGMSRAPATIRPTLLMLVILPFWTSFLIRVYAWI
GILKPEGLLNQLLLSLHIINQPLVILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDYSLIEAAKDLGCPPTAAFWKITFP
LSLPGVIAGCLLVFIPAVGEFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAVILLLLLTVPIMLFQQAQARAQEQ
GK

Sequences:

>Translated_322_residues
MSNIAVADAAAPPTAAKPLLTRLGAGFVNRLVIIVPYLWLLFFFLIPFVIVFKISLSQTAIAMPPYTPVLDFSDGISGFF
AGFRDLNFDNYVWLTQDALYFKAYVTSVIIAAISTVLTLIVGYPIAYGMSRAPATIRPTLLMLVILPFWTSFLIRVYAWI
GILKPEGLLNQLLLSLHIINQPLVILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDYSLIEAAKDLGCPPTAAFWKITFP
LSLPGVIAGCLLVFIPAVGEFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAVILLLLLTVPIMLFQQAQARAQEQ
GK
>Mature_321_residues
SNIAVADAAAPPTAAKPLLTRLGAGFVNRLVIIVPYLWLLFFFLIPFVIVFKISLSQTAIAMPPYTPVLDFSDGISGFFA
GFRDLNFDNYVWLTQDALYFKAYVTSVIIAAISTVLTLIVGYPIAYGMSRAPATIRPTLLMLVILPFWTSFLIRVYAWIG
ILKPEGLLNQLLLSLHIINQPLVILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDYSLIEAAKDLGCPPTAAFWKITFPL
SLPGVIAGCLLVFIPAVGEFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAVILLLLLTVPIMLFQQAQARAQEQG
K

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=322, Percent_Identity=53.7267080745342, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=42.7350427350427, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1787713, Length=211, Percent_Identity=27.9620853080569, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35522; Mature: 35391

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIAVADAAAPPTAAKPLLTRLGAGFVNRLVIIVPYLWLLFFFLIPFVIVFKISLSQTA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IAMPPYTPVLDFSDGISGFFAGFRDLNFDNYVWLTQDALYFKAYVTSVIIAAISTVLTLI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VGYPIAYGMSRAPATIRPTLLMLVILPFWTSFLIRVYAWIGILKPEGLLNQLLLSLHIIN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
QPLVILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDYSLIEAAKDLGCPPTAAFWKITFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
LSLPGVIAGCLLVFIPAVGEFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAVILL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHH
LLLTVPIMLFQQAQARAQEQGK
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNIAVADAAAPPTAAKPLLTRLGAGFVNRLVIIVPYLWLLFFFLIPFVIVFKISLSQTA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IAMPPYTPVLDFSDGISGFFAGFRDLNFDNYVWLTQDALYFKAYVTSVIIAAISTVLTLI
EECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHH
VGYPIAYGMSRAPATIRPTLLMLVILPFWTSFLIRVYAWIGILKPEGLLNQLLLSLHIIN
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
QPLVILNTYTAIFIGIVYSYLPFMVLPLYSSLEKMDYSLIEAAKDLGCPPTAAFWKITFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
LSLPGVIAGCLLVFIPAVGEFVIPDLLGGSQTLMIGKTLWNEFFANRDWPVSSAVAVILL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHH
LLLTVPIMLFQQAQARAQEQGK
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]