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The map label for this gene is ybiS [H]

Identifier: 13475412

GI number: 13475412

Start: 5285616

End: 5286206

Strand: Reverse

Name: ybiS [H]

Synonym: mll6473

Alternate gene names: 13475412

Gene position: 5286206-5285616 (Counterclockwise)

Preceding gene: 13475413

Following gene: 13475411

Centisome position: 75.13

GC content: 66.5

Gene sequence:

>591_bases
ATGACGCTGCCCGGTAGAGACGGCCTCGTCCCCAGGCTGGCCGGCACCTGCGTGCTCGGCCTGCTGCTGGCGATGTCCGC
CCAGGGCTTCGCCGAGGCGCGGTCGGGGCTTTCGGATCGGCCGCCTCTGCCGGCGATGGGACCCAGCCTGCGCAAGGCGG
TTTCCTTCGCGACCACCGAACAGCCGGGAACGATCATCATCCGCAAGAGCGAGAAGGCGCTTTACCTGGTGACCCGCCAG
GGCCAGGCATTGCGCTACCAGATCAGCGTCGGGCGTGACGGATTTGGCTGGACCGGGGTGGTGAAGGTCGGCGCCAAGAC
GGAATGGCCGCAGTGGCGCCCGCCCCGGGAAATGCGCGCGCGCCAGCCGGAACTGCCGGAACTGGTGCCCTCGGGTCCTT
ATAATCCGCTCGGCGCACGGGCGCTGTATCTGCTGCGCGACGGCCGCGACACGCTCTACCGTATCCATGGCACCAACGAT
CCCTCGGGCGTGGGATTCGATGGCACATCGGGCTGCTTTCGCCTTACCAACACGGATGTGATCGATCTTTTCAAGCGCGT
GCCAGTTGGCACCAAGGTGGTGGTTCAATGA

Upstream 100 bases:

>100_bases
CTTCTGACCGATGCCTTGAACCGGGCGATCCTGCCCTCCGAAGTGGATGGCCGGGCGCGCTTCAACAAGGATTTGAAGAA
GATGTTCAAAGAGCGGCTCG

Downstream 100 bases:

>100_bases
CGACGATCAGCGACCCGCATGTACCGACTGTCTCGGCCGAGCCGATTGAACCGCAACTGCTCGGCAGGGATGCGAGCCGG
TCGCGGCTGGCCGTCTTCGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MTLPGRDGLVPRLAGTCVLGLLLAMSAQGFAEARSGLSDRPPLPAMGPSLRKAVSFATTEQPGTIIIRKSEKALYLVTRQ
GQALRYQISVGRDGFGWTGVVKVGAKTEWPQWRPPREMRARQPELPELVPSGPYNPLGARALYLLRDGRDTLYRIHGTND
PSGVGFDGTSGCFRLTNTDVIDLFKRVPVGTKVVVQ

Sequences:

>Translated_196_residues
MTLPGRDGLVPRLAGTCVLGLLLAMSAQGFAEARSGLSDRPPLPAMGPSLRKAVSFATTEQPGTIIIRKSEKALYLVTRQ
GQALRYQISVGRDGFGWTGVVKVGAKTEWPQWRPPREMRARQPELPELVPSGPYNPLGARALYLLRDGRDTLYRIHGTND
PSGVGFDGTSGCFRLTNTDVIDLFKRVPVGTKVVVQ
>Mature_195_residues
TLPGRDGLVPRLAGTCVLGLLLAMSAQGFAEARSGLSDRPPLPAMGPSLRKAVSFATTEQPGTIIIRKSEKALYLVTRQG
QALRYQISVGRDGFGWTGVVKVGAKTEWPQWRPPREMRARQPELPELVPSGPYNPLGARALYLLRDGRDTLYRIHGTNDP
SGVGFDGTSGCFRLTNTDVIDLFKRVPVGTKVVVQ

Specific function: Responsible, at least in part, for anchoring of the major outer membrane lipoprotein (Lpp) to the peptidoglycan via a meso-diaminopimelyl-L-Lys- bond on the terminal residue of Lpp [H]

COG id: COG1376

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the YkuD family [H]

Homologues:

Organism=Escherichia coli, GI1787040, Length=138, Percent_Identity=39.8550724637681, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788299, Length=140, Percent_Identity=37.8571428571429, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI1787356, Length=139, Percent_Identity=36.6906474820144, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787968, Length=137, Percent_Identity=37.2262773722628, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005490 [H]

Pfam domain/function: PF03734 YkuD [H]

EC number: NA

Molecular weight: Translated: 21315; Mature: 21184

Theoretical pI: Translated: 10.64; Mature: 10.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPGRDGLVPRLAGTCVLGLLLAMSAQGFAEARSGLSDRPPLPAMGPSLRKAVSFATTE
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
QPGTIIIRKSEKALYLVTRQGQALRYQISVGRDGFGWTGVVKVGAKTEWPQWRPPREMRA
CCCEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHC
RQPELPELVPSGPYNPLGARALYLLRDGRDTLYRIHGTNDPSGVGFDGTSGCFRLTNTDV
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHH
IDLFKRVPVGTKVVVQ
HHHHHHCCCCEEEEEC
>Mature Secondary Structure 
TLPGRDGLVPRLAGTCVLGLLLAMSAQGFAEARSGLSDRPPLPAMGPSLRKAVSFATTE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCC
QPGTIIIRKSEKALYLVTRQGQALRYQISVGRDGFGWTGVVKVGAKTEWPQWRPPREMRA
CCCEEEEEECCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHC
RQPELPELVPSGPYNPLGARALYLLRDGRDTLYRIHGTNDPSGVGFDGTSGCFRLTNTDV
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHH
IDLFKRVPVGTKVVVQ
HHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]