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The map label for this gene is melA [H]

Identifier: 13475394

GI number: 13475394

Start: 5272082

End: 5273467

Strand: Direct

Name: melA [H]

Synonym: mlr6450

Alternate gene names: 13475394

Gene position: 5272082-5273467 (Clockwise)

Preceding gene: 13475393

Following gene: 13475401

Centisome position: 74.93

GC content: 62.91

Gene sequence:

>1386_bases
ATGCCGGAGGTTAGCATGGCAAGACATCCCAGGATCACTTTCATCGGCGCCGGCTCGACCGTGTTCATGAAGAACATTGT
CGGCGACGTGCTGCAGCGGCCGGCCCTCTCGGGCGCGACGATCGCGCTGATGGACATCAATCCGCAGCGGCTGGAAGAGA
GCGCCATCGTCGTCAACAAGCTGATCGCGACGCTCGGCGTGAAGGCGAAGGCCGAGACCTATTCCGACCAGCGCAAGGCC
CTTGCCGGGGCCGATTTCGTCGTCGTCGCCTTCCAGATCGGCGGCTACGAGCCCTGCACCATCACCGACTTCGAAGTGCC
GAAGAAATACGGCCTGCGCCAGACGATCGCCGACACGCTCGGCGTCGGCGGCATCATGCGCGGCCTCAGAACCGTGCCGC
ATCTGTGGAAGATCTGCGAGGACATGCTCGCCGTCTGCCCCGAAGCGATCATGCTGCAATATGTCAACCCGATGGCGATC
AACACCTGGGCGATCTCGGAGAAATACCCCACCATCAAGCAGGTCGGGCTCTGTCACTCGGTGCAGGGCACGGCGATGGA
ACTCGCTCACGACCTCTCGCTGCCGTATGACGAGATCCGCTATCGGTCGGCCGGCATCAACCACATGGCCTTCTACCTGA
AATTCGAGCACCGCCAGCCCGACGGCTCCTACCGCGACCTCTATCCGGATCTCGTGCGCGCCTATCGCGAAGGCCGCGCG
CCCAAGCCGGGCTGGAATCCACGCTGCCCCAACAAGGTACGCTACGAGATGCTGACCAGGCTCGGCTATTTCGTCACCGA
AAGCTCGGAGCATTTCGCCGAGTACACGCCCTATTTCATCAAGGAGGGTCGCCCCGATCTGATCGAGAAATACGGCATTC
CGCTCGACGAATACCCCAAGCGCTGCATCGAGCAGATCGAGCGCTGGAAAGACCAGGCGCAGGCCTACCGCTCGGCCCAG
CGCATCGAGGTCGAGGAGTCCAGGGAGTATGCCTCCTCGATCATGAACTCGGTCTGGACCGGCGAACCGTCGGTGATCTA
CGGCAATGTCCGCAACAATGGCTGCATCACCTCGCTGCCCCGCGACTGCGCCGCCGAAGTGCCCTGCCTTGTCGATGCCT
CCGGCATTCAGCCGACCTATATAGGCGATCTGCCGCCGCAGCTGACGGCGCTGATCCGCACCAACATCAATGTCCAGGAA
CTGACGGTGCGGGCTCTGATGAGCGAGAACCGCGAGCACATTTACCATGCGGCGATGATGGATCCGCACACGGCGGCCGA
ACTCGACCTCGACCAGATCTGCTCACTGGTCGATGATCTCCTGGCCGCTCACGGCGACTGGCTACCGGCCTGGGCGCGCG
TGAGCCGCAAGACCGAGGCCGCCTGA

Upstream 100 bases:

>100_bases
AAGATCGTCGAGCAGGGACCCGTCGACGAGATCCTCACAAATCCCAAGACCGCCTACACCGAACGGCTCGTCCGAGCCGC
TTTCGACGTGGCTTGAACAT

Downstream 100 bases:

>100_bases
CGGGCGATCTCGGTCTTACTTGGGCTCGTCGGCGTCGGGTTCTTCCTCGTCGTCGCGGGCCTTGATGACATAGGCGCCCT
TCAGCCAGCGGTTGAGGTCG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPEVSMARHPRITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNKLIATLGVKAKAETYSDQRKA
LAGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTLGVGGIMRGLRTVPHLWKICEDMLAVCPEAIMLQYVNPMAI
NTWAISEKYPTIKQVGLCHSVQGTAMELAHDLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRA
PKPGWNPRCPNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPKRCIEQIERWKDQAQAYRSAQ
RIEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLPRDCAAEVPCLVDASGIQPTYIGDLPPQLTALIRTNINVQE
LTVRALMSENREHIYHAAMMDPHTAAELDLDQICSLVDDLLAAHGDWLPAWARVSRKTEAA

Sequences:

>Translated_461_residues
MPEVSMARHPRITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNKLIATLGVKAKAETYSDQRKA
LAGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTLGVGGIMRGLRTVPHLWKICEDMLAVCPEAIMLQYVNPMAI
NTWAISEKYPTIKQVGLCHSVQGTAMELAHDLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRA
PKPGWNPRCPNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPKRCIEQIERWKDQAQAYRSAQ
RIEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLPRDCAAEVPCLVDASGIQPTYIGDLPPQLTALIRTNINVQE
LTVRALMSENREHIYHAAMMDPHTAAELDLDQICSLVDDLLAAHGDWLPAWARVSRKTEAA
>Mature_460_residues
PEVSMARHPRITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNKLIATLGVKAKAETYSDQRKAL
AGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTLGVGGIMRGLRTVPHLWKICEDMLAVCPEAIMLQYVNPMAIN
TWAISEKYPTIKQVGLCHSVQGTAMELAHDLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRAP
KPGWNPRCPNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPKRCIEQIERWKDQAQAYRSAQR
IEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLPRDCAAEVPCLVDASGIQPTYIGDLPPQLTALIRTNINVQEL
TVRALMSENREHIYHAAMMDPHTAAELDLDQICSLVDDLLAAHGDWLPAWARVSRKTEAA

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=448, Percent_Identity=66.5178571428571, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=458, Percent_Identity=23.1441048034935, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 51819; Mature: 51688

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVSMARHPRITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNK
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHH
LIATLGVKAKAETYSDQRKALAGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTL
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCCCCHHHCHHHHHHHHH
GVGGIMRGLRTVPHLWKICEDMLAVCPEAIMLQYVNPMAINTWAISEKYPTIKQVGLCHS
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHC
VQGTAMELAHDLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRA
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
PKPGWNPRCPNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHCCCHHHHHH
RCIEQIERWKDQAQAYRSAQRIEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
RDCAAEVPCLVDASGIQPTYIGDLPPQLTALIRTNINVQELTVRALMSENREHIYHAAMM
HHHHHCCCCEEECCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHC
DPHTAAELDLDQICSLVDDLLAAHGDWLPAWARVSRKTEAA
CCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PEVSMARHPRITFIGAGSTVFMKNIVGDVLQRPALSGATIALMDINPQRLEESAIVVNK
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHH
LIATLGVKAKAETYSDQRKALAGADFVVVAFQIGGYEPCTITDFEVPKKYGLRQTIADTL
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEECCCCCCHHHCHHHHHHHHH
GVGGIMRGLRTVPHLWKICEDMLAVCPEAIMLQYVNPMAINTWAISEKYPTIKQVGLCHS
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHC
VQGTAMELAHDLSLPYDEIRYRSAGINHMAFYLKFEHRQPDGSYRDLYPDLVRAYREGRA
CCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCC
PKPGWNPRCPNKVRYEMLTRLGYFVTESSEHFAEYTPYFIKEGRPDLIEKYGIPLDEYPK
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHCCCCHHHHHHHCCCHHHHHH
RCIEQIERWKDQAQAYRSAQRIEVEESREYASSIMNSVWTGEPSVIYGNVRNNGCITSLP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
RDCAAEVPCLVDASGIQPTYIGDLPPQLTALIRTNINVQELTVRALMSENREHIYHAAMM
HHHHHCCCCEEECCCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHC
DPHTAAELDLDQICSLVDDLLAAHGDWLPAWARVSRKTEAA
CCCCHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]