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The map label for this gene is 13475255

Identifier: 13475255

GI number: 13475255

Start: 5098762

End: 5099604

Strand: Direct

Name: 13475255

Synonym: mlr6283

Alternate gene names: NA

Gene position: 5098762-5099604 (Clockwise)

Preceding gene: 13475254

Following gene: 13475258

Centisome position: 72.47

GC content: 50.77

Gene sequence:

>843_bases
ATGACAACGCGGATATTGGGTGTGGTCCAGCTTGATCAAAGGCGACTGACAGACGATTTGGCTGTCTTAGCGAAGTCCAA
CTTCTCGAGCGAATATTCGGATTTCGCCTGCGGGCGGTGGGAATTCTGCATGCTCCGCAATCAGTCGGGGAAGCAGGAGG
AGCAGAGAGTGGTCGTCCACGAGACCCCAGCGCTGGCGACACCTCTGGGCCAATCCTTACCCTATCTCAATGAATTGTTG
GACAATCACTTTGATAGGGACTCTATACGCTACGCGCGGATCATCCGGATATCAGAAAACGCGTGTATAATACCTCACCG
TGATTACTTGGAACTAGAAGGGAAATTTATCAGAGTGCACCTAGTTCTAGATACGAATGAAAAGTGTTCCAATACAGAAG
AGAATAATATATTCCATATGGGACGAGGTGAGATCTGGTTTCTTGACGCAAGCCTGCCGCACAGCGCGGGATGTTTCTCA
CCAACTCCACGCTTACATCTAGTGGTCGACATCGAGGGGACTCGTTCCCTGGAAGAGGTTGCAATCAATGTCGAACAGCC
GTCGGCAAGGAATGCCACGGTGGATACTCGCAAGGAGTGGACTGATGAAACGCTCGAATCCGTTCTGGGATTTTCGGAGA
TTATCAGCGAGGCCAATTATCGAGAGATCGTCGCGATTCTGGCGAAGCTCCACTTTTTCCACAAGGTCCACTGCGTGGAT
ATGTATGGCTGGCTTAAGGAAATCTGCCGACGTCGTGGCGAGCCGGCGCTTATAGAAAAGGCCAACTCGCTTGAGCGATT
TTATCTCATTGACCGTGCTGCTGGCGAGGTCATGACTTATTGA

Upstream 100 bases:

>100_bases
GGGAGGAGGGCAGGAACGAGGCACAACGCGCATATCCCCTTGGATAAGTCGCGCAGATAGCTGCCTGATTTTCGAAGTGA
ACTAATTCGGAGGCTAGACA

Downstream 100 bases:

>100_bases
TCGGTGTGACTGAGTTGTCCGTCGGGGGCGAGTAGTTGCTTTCCGCGCACCAAGCCGAGGATTCACGTAGGGCAGTGGTC
AATGCCGGGGAAAATATGGC

Product: L-proline 3-hydroxylase

Products: NA

Alternate protein names: P4H

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTTRILGVVQLDQRRLTDDLAVLAKSNFSSEYSDFACGRWEFCMLRNQSGKQEEQRVVVHETPALATPLGQSLPYLNELL
DNHFDRDSIRYARIIRISENACIIPHRDYLELEGKFIRVHLVLDTNEKCSNTEENNIFHMGRGEIWFLDASLPHSAGCFS
PTPRLHLVVDIEGTRSLEEVAINVEQPSARNATVDTRKEWTDETLESVLGFSEIISEANYREIVAILAKLHFFHKVHCVD
MYGWLKEICRRRGEPALIEKANSLERFYLIDRAAGEVMTY

Sequences:

>Translated_280_residues
MTTRILGVVQLDQRRLTDDLAVLAKSNFSSEYSDFACGRWEFCMLRNQSGKQEEQRVVVHETPALATPLGQSLPYLNELL
DNHFDRDSIRYARIIRISENACIIPHRDYLELEGKFIRVHLVLDTNEKCSNTEENNIFHMGRGEIWFLDASLPHSAGCFS
PTPRLHLVVDIEGTRSLEEVAINVEQPSARNATVDTRKEWTDETLESVLGFSEIISEANYREIVAILAKLHFFHKVHCVD
MYGWLKEICRRRGEPALIEKANSLERFYLIDRAAGEVMTY
>Mature_279_residues
TTRILGVVQLDQRRLTDDLAVLAKSNFSSEYSDFACGRWEFCMLRNQSGKQEEQRVVVHETPALATPLGQSLPYLNELLD
NHFDRDSIRYARIIRISENACIIPHRDYLELEGKFIRVHLVLDTNEKCSNTEENNIFHMGRGEIWFLDASLPHSAGCFSP
TPRLHLVVDIEGTRSLEEVAINVEQPSARNATVDTRKEWTDETLESVLGFSEIISEANYREIVAILAKLHFFHKVHCVDM
YGWLKEICRRRGEPALIEKANSLERFYLIDRAAGEVMTY

Specific function: Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-4-hydroxy-L- proline (cis-4-Hyp)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L-proline cis-4-/cis-3-hydroxylase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P4H_RHILO (Q989T9)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106819.1
- HSSP:   O09345
- ProteinModelPortal:   Q989T9
- SMR:   Q989T9
- GeneID:   1229474
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr6283
- NMPDR:   fig|266835.1.peg.4920
- HOGENOM:   HBG211626
- OMA:   ISEANYR
- ProtClustDB:   CLSK506560
- InterPro:   IPR007803
- InterPro:   IPR008035
- Gene3D:   G3DSA:1.10.1720.10

Pfam domain/function: PF05118 Asp_Arg_Hydrox; PF05373 Pro_3_hydrox_C

EC number: NA

Molecular weight: Translated: 32217; Mature: 32086

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRILGVVQLDQRRLTDDLAVLAKSNFSSEYSDFACGRWEFCMLRNQSGKQEEQRVVVH
CCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHCEEEEE
ETPALATPLGQSLPYLNELLDNHFDRDSIRYARIIRISENACIIPHRDYLELEGKFIRVH
CCCCHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCEEEECCCCCEEECCEEEEEE
LVLDTNEKCSNTEENNIFHMGRGEIWFLDASLPHSAGCFSPTPRLHLVVDIEGTRSLEEV
EEEECCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
AINVEQPSARNATVDTRKEWTDETLESVLGFSEIISEANYREIVAILAKLHFFHKVHCVD
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MYGWLKEICRRRGEPALIEKANSLERFYLIDRAAGEVMTY
HHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TTRILGVVQLDQRRLTDDLAVLAKSNFSSEYSDFACGRWEFCMLRNQSGKQEEQRVVVH
CCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHCEEEEE
ETPALATPLGQSLPYLNELLDNHFDRDSIRYARIIRISENACIIPHRDYLELEGKFIRVH
CCCCHHCCCCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCEEEECCCCCEEECCEEEEEE
LVLDTNEKCSNTEENNIFHMGRGEIWFLDASLPHSAGCFSPTPRLHLVVDIEGTRSLEEV
EEEECCCCCCCCCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHH
AINVEQPSARNATVDTRKEWTDETLESVLGFSEIISEANYREIVAILAKLHFFHKVHCVD
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
MYGWLKEICRRRGEPALIEKANSLERFYLIDRAAGEVMTY
HHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968