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The map label for this gene is potD [C]

Identifier: 13475229

GI number: 13475229

Start: 5076721

End: 5077836

Strand: Reverse

Name: potD [C]

Synonym: mll6256

Alternate gene names: 13475229

Gene position: 5077836-5076721 (Counterclockwise)

Preceding gene: 13475233

Following gene: 13475228

Centisome position: 72.17

GC content: 56.09

Gene sequence:

>1116_bases
ATGAAATATTTTATCAACTCAAGCAGACCTACCCGGCGTCAAATGCTTACGGGAATGGGTGTCGCGGCCGGTGGTGCAGC
GCTCGCAATTGGCGCTCCCGGCATCTTGAGGGCGCAGGAATCCCCTCTCGTTGTAGTCTCCGGTGGCGGATCGTACGAGG
ACGCGATCCGCAAAGCGATTATTGAGCCCTTCCAAAAAGAGACCGGTATCAGTGTGACTTATGTGACTCCGGCCAGCGAG
GCGAAACTCAAGGCGCAGGTCGATAGCAACAACGTACAGTGGGATGTGATGGAGCTATCCAATCCCGCTTTTCGACCCGA
TGCGAATAAGTATCTCGAGGAAATCGACTATGCGGCGTTCGACAAGGAAACGTTGGACAACCTCGTTCCTGAAGCAAAGA
CGAAATATGGGGTAGGCTCGTTCGTCTATAGCACGGTGATGGCGTTCTCCACGGTCGAATACCCGGTTGACAAGCCTCGC
CCGACCTCCTGGGCCGAGTTTTGGGATATTAGCAAGTTTCCTGGGCCGCGCACGCTTGAAACATGTGCCGATGGTAGCAC
TGCCACGGTAGAATTCGCGTTGCTGGCCGCTGGTGTGCCGAAGGACAAGCTGTACCCAATTGACCTCGACAAGGCATTCG
CGTCCCTCGATCGCATCAAACCGCAGATTGCGAAATTCTGGAGTGTGGGAGCCGAACCGGGCCAAATGCTGTCCGATCGT
CAGGTCGCCATGGGCTCGGCCTGGAACGGCCGTATGCAAGCGCTCAAGGATCAGGGCGCTAGCATTGATTACCAGTGGAA
CGAAGCGCAGCTTAGCCCGACCCACTGGGTTGTGCCCAAGGGCGCGAAAAACCGGGCCGCGGCCATGAAGTTTGCAGCCT
ACCAAGCCTCGGCCCAAGCGCAGGCTCGCCTCAACAATCTCATTTTTTATGGACCGCTCAATCGCGCCGCTTTCAAATAC
ATCGAACCTAAGGTTGCCGCGCAACTGCCCACGGCGCCCGACAATCTAAACAAACAATATTTTGCCAATCTGGAATACTG
GCAGACCCAGTCCTCTTCCGGCAAGAGTAATACCGACATCCTGGTCGATCGCTGGGCCGAGTGGGTGGCTAGTTAG

Upstream 100 bases:

>100_bases
TCGAGCCTAATCGACATAATCGGGTCGGCTCCTTTACGCGCCGCCACATATTCAGCCCGAATCGCGATCGAGTAATATTA
ACAAGCAAGGGAGAGAACTC

Downstream 100 bases:

>100_bases
CACCACTTTGTAATACGCCTTGTGGGCGGCCATAGCGTGGCCGCTCACATCAGGCGAAGCGCCTTGATTGATATTGAGGC
GCTGCTGTTCCGGATCTTTC

Product: ABC transporter binding protein component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKYFINSSRPTRRQMLTGMGVAAGGAALAIGAPGILRAQESPLVVVSGGGSYEDAIRKAIIEPFQKETGISVTYVTPASE
AKLKAQVDSNNVQWDVMELSNPAFRPDANKYLEEIDYAAFDKETLDNLVPEAKTKYGVGSFVYSTVMAFSTVEYPVDKPR
PTSWAEFWDISKFPGPRTLETCADGSTATVEFALLAAGVPKDKLYPIDLDKAFASLDRIKPQIAKFWSVGAEPGQMLSDR
QVAMGSAWNGRMQALKDQGASIDYQWNEAQLSPTHWVVPKGAKNRAAAMKFAAYQASAQAQARLNNLIFYGPLNRAAFKY
IEPKVAAQLPTAPDNLNKQYFANLEYWQTQSSSGKSNTDILVDRWAEWVAS

Sequences:

>Translated_371_residues
MKYFINSSRPTRRQMLTGMGVAAGGAALAIGAPGILRAQESPLVVVSGGGSYEDAIRKAIIEPFQKETGISVTYVTPASE
AKLKAQVDSNNVQWDVMELSNPAFRPDANKYLEEIDYAAFDKETLDNLVPEAKTKYGVGSFVYSTVMAFSTVEYPVDKPR
PTSWAEFWDISKFPGPRTLETCADGSTATVEFALLAAGVPKDKLYPIDLDKAFASLDRIKPQIAKFWSVGAEPGQMLSDR
QVAMGSAWNGRMQALKDQGASIDYQWNEAQLSPTHWVVPKGAKNRAAAMKFAAYQASAQAQARLNNLIFYGPLNRAAFKY
IEPKVAAQLPTAPDNLNKQYFANLEYWQTQSSSGKSNTDILVDRWAEWVAS
>Mature_371_residues
MKYFINSSRPTRRQMLTGMGVAAGGAALAIGAPGILRAQESPLVVVSGGGSYEDAIRKAIIEPFQKETGISVTYVTPASE
AKLKAQVDSNNVQWDVMELSNPAFRPDANKYLEEIDYAAFDKETLDNLVPEAKTKYGVGSFVYSTVMAFSTVEYPVDKPR
PTSWAEFWDISKFPGPRTLETCADGSTATVEFALLAAGVPKDKLYPIDLDKAFASLDRIKPQIAKFWSVGAEPGQMLSDR
QVAMGSAWNGRMQALKDQGASIDYQWNEAQLSPTHWVVPKGAKNRAAAMKFAAYQASAQAQARLNNLIFYGPLNRAAFKY
IEPKVAAQLPTAPDNLNKQYFANLEYWQTQSSSGKSNTDILVDRWAEWVAS

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40751; Mature: 40751

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYFINSSRPTRRQMLTGMGVAAGGAALAIGAPGILRAQESPLVVVSGGGSYEDAIRKAI
CCEEECCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCEEEEECCCCHHHHHHHHH
IEPFQKETGISVTYVTPASEAKLKAQVDSNNVQWDVMELSNPAFRPDANKYLEEIDYAAF
HHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
DKETLDNLVPEAKTKYGVGSFVYSTVMAFSTVEYPVDKPRPTSWAEFWDISKFPGPRTLE
CHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHH
TCADGSTATVEFALLAAGVPKDKLYPIDLDKAFASLDRIKPQIAKFWSVGAEPGQMLSDR
HHCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
QVAMGSAWNGRMQALKDQGASIDYQWNEAQLSPTHWVVPKGAKNRAAAMKFAAYQASAQA
HHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
QARLNNLIFYGPLNRAAFKYIEPKVAAQLPTAPDNLNKQYFANLEYWQTQSSSGKSNTDI
HHHHCCEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCH
LVDRWAEWVAS
HHHHHHHHHCC
>Mature Secondary Structure
MKYFINSSRPTRRQMLTGMGVAAGGAALAIGAPGILRAQESPLVVVSGGGSYEDAIRKAI
CCEEECCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCEEEEECCCCHHHHHHHHH
IEPFQKETGISVTYVTPASEAKLKAQVDSNNVQWDVMELSNPAFRPDANKYLEEIDYAAF
HHHHHHHCCCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHC
DKETLDNLVPEAKTKYGVGSFVYSTVMAFSTVEYPVDKPRPTSWAEFWDISKFPGPRTLE
CHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHH
TCADGSTATVEFALLAAGVPKDKLYPIDLDKAFASLDRIKPQIAKFWSVGAEPGQMLSDR
HHCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCC
QVAMGSAWNGRMQALKDQGASIDYQWNEAQLSPTHWVVPKGAKNRAAAMKFAAYQASAQA
HHHCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHH
QARLNNLIFYGPLNRAAFKYIEPKVAAQLPTAPDNLNKQYFANLEYWQTQSSSGKSNTDI
HHHHCCEEEECCCCHHHHHHCCCHHHHCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCH
LVDRWAEWVAS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA