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The map label for this gene is pgtE [H]

Identifier: 13475122

GI number: 13475122

Start: 4963904

End: 4964872

Strand: Direct

Name: pgtE [H]

Synonym: mlr6132

Alternate gene names: 13475122

Gene position: 4963904-4964872 (Clockwise)

Preceding gene: 13475121

Following gene: 13475127

Centisome position: 70.55

GC content: 60.27

Gene sequence:

>969_bases
ATGAAATTGTACGCGAAAGTCTTTGGTGGAGCGGCAGCGTTGCTCGCTGCCTGGCCGGCGATGGCCAGCGACGCGGTGGC
GAGCGAACCGGCGCCCTACAATCCCAATTTCTCTTTCGTTGGCGGCGTCGGCGTCATCGACATCCAGGCCAACGAGCTGG
TCTATCAGGCTCCCGGCAGCGGCTCGAAGCTGAGCCACCTTATCTGGGAATCCACCTCTGCGGTCATTTCAGCCGAGATG
ACGGCGCGCAGCGAGGCCGGCTGGACCGCCAAGCTTTCCGGCCAGATCGCCTTCAGCGGCAACAGCTACATGGAAGACTA
TGACTGGAACCCGTCATTCTCGACCAATGATGGCTGGAACGACTGGAGCAACCGTTCGCGCCACTGCGACACCGATCTTG
ATTACTTCTATTCGGCAACCGTCGCGCTCGGCCACGATTTCCAGTTCAGCGACCAGCTGCTGATCAACGTCAACGGTGGT
TTCAAGTACACCTCGGTCAAGTGGACTGCCTATGGCGGCTCCTATGTCTACAGCGTTGCCGGCTTCCGGGATTCGGTTGG
CGACTTCCCAGGAGACGCAAAGGCCATCAGCTACCAGCAGAAATTGCCGGTCGCCTTCGCCGGCATCGATGCGACCTATG
TCCAGGATCGCTGGAACTTCGGCTTTGCGCTGAAGGGCGGCACGACCTTCTCGGCCGGCGCTACCGATCATCACTGGATG
CGTGACCTGCGCTTCGAGGATGATTTCGAATCGGCGCCGGTTCTGATGGTCGGCGGCAGCGTCGGCTACCAGTACAACGA
GCGGACCAGCTTCTTCGTCTCCGGTTCCTACGTAAAGGTGTTCACCGCTCGCGGCGACTACAACACCTATGACATTGCGA
CCGGCGCAGAGACTGGCGGCGAAGACGACTGGATCGGCGGCGATTTTGCCGCGGCGACAGTGATGGTCGGCATCAAGACC
TCGTTCTGA

Upstream 100 bases:

>100_bases
TGCCAACCTACAGGGGCGCTGCATAGACGACCGCACCTGACGTGCACCCGGTTACGCTGCGTATAACCAAATGGCGGATC
ATTCCCATGAGGGTAGTCCT

Downstream 100 bases:

>100_bases
TTGAACGAGTGCGACCGGAACGCCAAACCGATGTCCGCACGGCGCAGAAATCTGACGCGCCCGCGCGCCTGACGGCGCGT
GCCGGGTGCTCGTGAACCGA

Product: outer membrane protease

Products: NA

Alternate protein names: E protein [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKLYAKVFGGAAALLAAWPAMASDAVASEPAPYNPNFSFVGGVGVIDIQANELVYQAPGSGSKLSHLIWESTSAVISAEM
TARSEAGWTAKLSGQIAFSGNSYMEDYDWNPSFSTNDGWNDWSNRSRHCDTDLDYFYSATVALGHDFQFSDQLLINVNGG
FKYTSVKWTAYGGSYVYSVAGFRDSVGDFPGDAKAISYQQKLPVAFAGIDATYVQDRWNFGFALKGGTTFSAGATDHHWM
RDLRFEDDFESAPVLMVGGSVGYQYNERTSFFVSGSYVKVFTARGDYNTYDIATGAETGGEDDWIGGDFAAATVMVGIKT
SF

Sequences:

>Translated_322_residues
MKLYAKVFGGAAALLAAWPAMASDAVASEPAPYNPNFSFVGGVGVIDIQANELVYQAPGSGSKLSHLIWESTSAVISAEM
TARSEAGWTAKLSGQIAFSGNSYMEDYDWNPSFSTNDGWNDWSNRSRHCDTDLDYFYSATVALGHDFQFSDQLLINVNGG
FKYTSVKWTAYGGSYVYSVAGFRDSVGDFPGDAKAISYQQKLPVAFAGIDATYVQDRWNFGFALKGGTTFSAGATDHHWM
RDLRFEDDFESAPVLMVGGSVGYQYNERTSFFVSGSYVKVFTARGDYNTYDIATGAETGGEDDWIGGDFAAATVMVGIKT
SF
>Mature_322_residues
MKLYAKVFGGAAALLAAWPAMASDAVASEPAPYNPNFSFVGGVGVIDIQANELVYQAPGSGSKLSHLIWESTSAVISAEM
TARSEAGWTAKLSGQIAFSGNSYMEDYDWNPSFSTNDGWNDWSNRSRHCDTDLDYFYSATVALGHDFQFSDQLLINVNGG
FKYTSVKWTAYGGSYVYSVAGFRDSVGDFPGDAKAISYQQKLPVAFAGIDATYVQDRWNFGFALKGGTTFSAGATDHHWM
RDLRFEDDFESAPVLMVGGSVGYQYNERTSFFVSGSYVKVFTARGDYNTYDIATGAETGGEDDWIGGDFAAATVMVGIKT
SF

Specific function: Protease that can cleave T7 RNA polymerase. Specific for paired basic residues [H]

COG id: COG4571

COG function: function code M; Outer membrane protease

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A26 family [H]

Homologues:

Organism=Escherichia coli, GI1786777, Length=301, Percent_Identity=25.5813953488372, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020080
- InterPro:   IPR000036
- InterPro:   IPR020079 [H]

Pfam domain/function: PF01278 Omptin [H]

EC number: 3.4.23.- [C]

Molecular weight: Translated: 34927; Mature: 34927

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYAKVFGGAAALLAAWPAMASDAVASEPAPYNPNFSFVGGVGVIDIQANELVYQAPGS
CEEEEEHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCEEEECCEEEEEEECCEEEEECCCC
GSKLSHLIWESTSAVISAEMTARSEAGWTAKLSGQIAFSGNSYMEDYDWNPSFSTNDGWN
CCHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC
DWSNRSRHCDTDLDYFYSATVALGHDFQFSDQLLINVNGGFKYTSVKWTAYGGSYVYSVA
CCCCCCCCCCCCCHHHEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEC
GFRDSVGDFPGDAKAISYQQKLPVAFAGIDATYVQDRWNFGFALKGGTTFSAGATDHHWM
CCCHHHCCCCCCCCEEEHHHCCCEEEECCCEEEEECCCCCCEEEECCCEEECCCCCHHHH
RDLRFEDDFESAPVLMVGGSVGYQYNERTSFFVSGSYVKVFTARGDYNTYDIATGAETGG
HHCCCCCCCCCCCEEEECCCCCCEECCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCC
EDDWIGGDFAAATVMVGIKTSF
CCCCCCCCCEEEEEEEEEEECC
>Mature Secondary Structure
MKLYAKVFGGAAALLAAWPAMASDAVASEPAPYNPNFSFVGGVGVIDIQANELVYQAPGS
CEEEEEHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCEEEECCEEEEEEECCEEEEECCCC
GSKLSHLIWESTSAVISAEMTARSEAGWTAKLSGQIAFSGNSYMEDYDWNPSFSTNDGWN
CCHHHHHHHCCCCCEEEEEECCCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC
DWSNRSRHCDTDLDYFYSATVALGHDFQFSDQLLINVNGGFKYTSVKWTAYGGSYVYSVA
CCCCCCCCCCCCCHHHEEEEEEECCCCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEC
GFRDSVGDFPGDAKAISYQQKLPVAFAGIDATYVQDRWNFGFALKGGTTFSAGATDHHWM
CCCHHHCCCCCCCCEEEHHHCCCEEEECCCEEEEECCCCCCEEEECCCEEECCCCCHHHH
RDLRFEDDFESAPVLMVGGSVGYQYNERTSFFVSGSYVKVFTARGDYNTYDIATGAETGG
HHCCCCCCCCCCCEEEECCCCCCEECCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCC
EDDWIGGDFAAATVMVGIKTSF
CCCCCCCCCEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3023201; 10869088; 11677609; 2651422 [H]