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The map label for this gene is traI [H]

Identifier: 13475097

GI number: 13475097

Start: 4941996

End: 4942637

Strand: Direct

Name: traI [H]

Synonym: mlr6103

Alternate gene names: 13475097

Gene position: 4941996-4942637 (Clockwise)

Preceding gene: 13475096

Following gene: 13475099

Centisome position: 70.24

GC content: 61.37

Gene sequence:

>642_bases
ATGATTGAGCTTATTGCGCCCGGCTGGTACGGCGCGTTTGCTGACGAACTTCACGAAATGCACCGCCTGCGCTACCGCGT
CTTTAAGGAACGGCTGGACTGGAACGTGCGCACGACCGGCGGCTTCGAGATCGATTCATTCGATTCCCTGAAACCGCACT
ATCTCGTGTTGCGCGATTCCGCCGGTCGCGTCAGGGGCGGCGTACGCCTTCTGCCCTCGACGGGGCCGACGATGCTGCGA
GATGTCTTTTCGAGGCTGTTGGAAGGGAGAGCGGCACCCGAGGAACCGAGTGTCTGGGAAAGCAGCCGTTTCGCCCTTGA
TCTCCCACCGTCTGCACCGAAGGACAGTGGGAGTATAGCTGTTGCCACCTATGAACTTCTCGCTGGCATGATCGAGTTCG
GTTTGTCACGGCTACTCACCCATATCGTCACGGTTACGGATTTGCGCATGGAGCGAATCCTGCGTCGCGCCGGGTGGCCG
CTGGACCGGATCGGTCCGCCCCAGACAATCGGCACCACGTGCGCCGTTGCCGGCTGTTTGGATGTCTCAGAGGAGAGCCT
TGCTGCGGTCCGCCACAATGGCGGTCTTGGCGGTCCCGTACTGTGGGCCGGTGCTCTGCACGGGCGCCTGACATGGTTAT
GA

Upstream 100 bases:

>100_bases
GGGACAGAATCCCTTTTTGATCTACCTGTACAAATGCACAGGCAGTCGCGGCATGAAGGTTCGCCTCCAATGGCAGGGTC
ATACCTGCGAGGAGACCATC

Downstream 100 bases:

>100_bases
TAGGGAATGACGCCGACTGGCCGGACTGGCAAGATGAGAAATCCTATCGCTACACGCGGCACCTGACGCGACGCGGCTGG
GCCTGGGAATTCCTACGGCG

Product: conjugation factor synthetase, traI

Products: NA

Alternate protein names: Autoinducer synthesis protein traI [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MIELIAPGWYGAFADELHEMHRLRYRVFKERLDWNVRTTGGFEIDSFDSLKPHYLVLRDSAGRVRGGVRLLPSTGPTMLR
DVFSRLLEGRAAPEEPSVWESSRFALDLPPSAPKDSGSIAVATYELLAGMIEFGLSRLLTHIVTVTDLRMERILRRAGWP
LDRIGPPQTIGTTCAVAGCLDVSEESLAAVRHNGGLGGPVLWAGALHGRLTWL

Sequences:

>Translated_213_residues
MIELIAPGWYGAFADELHEMHRLRYRVFKERLDWNVRTTGGFEIDSFDSLKPHYLVLRDSAGRVRGGVRLLPSTGPTMLR
DVFSRLLEGRAAPEEPSVWESSRFALDLPPSAPKDSGSIAVATYELLAGMIEFGLSRLLTHIVTVTDLRMERILRRAGWP
LDRIGPPQTIGTTCAVAGCLDVSEESLAAVRHNGGLGGPVLWAGALHGRLTWL
>Mature_213_residues
MIELIAPGWYGAFADELHEMHRLRYRVFKERLDWNVRTTGGFEIDSFDSLKPHYLVLRDSAGRVRGGVRLLPSTGPTMLR
DVFSRLLEGRAAPEEPSVWESSRFALDLPPSAPKDSGSIAVATYELLAGMIEFGLSRLLTHIVTVTDLRMERILRRAGWP
LDRIGPPQTIGTTCAVAGCLDVSEESLAAVRHNGGLGGPVLWAGALHGRLTWL

Specific function: Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L- homoserine lactone), an autoinducer molecule which binds to traR and thus acts in the control of conjugal transfer [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 23515; Mature: 23515

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00949 AUTOINDUCERS_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELIAPGWYGAFADELHEMHRLRYRVFKERLDWNVRTTGGFEIDSFDSLKPHYLVLRDS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECC
AGRVRGGVRLLPSTGPTMLRDVFSRLLEGRAAPEEPSVWESSRFALDLPPSAPKDSGSIA
CCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEE
VATYELLAGMIEFGLSRLLTHIVTVTDLRMERILRRAGWPLDRIGPPQTIGTTCAVAGCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVSEESLAAVRHNGGLGGPVLWAGALHGRLTWL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCEEECC
>Mature Secondary Structure
MIELIAPGWYGAFADELHEMHRLRYRVFKERLDWNVRTTGGFEIDSFDSLKPHYLVLRDS
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEEECC
AGRVRGGVRLLPSTGPTMLRDVFSRLLEGRAAPEEPSVWESSRFALDLPPSAPKDSGSIA
CCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEE
VATYELLAGMIEFGLSRLLTHIVTVTDLRMERILRRAGWPLDRIGPPQTIGTTCAVAGCL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
DVSEESLAAVRHNGGLGGPVLWAGALHGRLTWL
CCCHHHHHHHHHCCCCCCHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8197112; 9829924; 11743193; 11743194 [H]