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The map label for this gene is fumB [H]

Identifier: 13475093

GI number: 13475093

Start: 4936817

End: 4938361

Strand: Direct

Name: fumB [H]

Synonym: mlr6099

Alternate gene names: 13475093

Gene position: 4936817-4938361 (Clockwise)

Preceding gene: 13475092

Following gene: 13475094

Centisome position: 70.16

GC content: 59.22

Gene sequence:

>1545_bases
GTGGGAAATAGAACACGGACCATAGCCGGCAAGGACATCACAAGGAGCGTGGCCGACGCCCTTCAGTACATCTCGTACTA
CCATCCTCCAGATTATATCCGGTCTCTTTCGCAGGCCTACGCGCGCGAACAGAGTCCAGCGGCGAAGAACGCAATAGGGC
AGATTCTGCTGAACTCCCGCATGGCGGCCTTCGGGCGGCGGCCGATCTGCCAGGACACCGGCCTTGTGGTCGTCTTCGCG
AAGGTCGGCATGGAAACCCGCATCAAATCAACGATCAGTTTCGCAGACCTCGTGAACGAGGGCGTCCGTCAGGCATATCT
CGACCCGGACAATCCCCTTCGGGCCTCGATCGTTGCCGATCCGCTCGCCGGACGGGTCAATACTCGTGACAACACGCCGG
CGGTCGTCCACGTTGACCTTGTACAAGGTAACCAGATTGAGATCACCATTGCCGCAAAAGGCGGCGGGTCAGAGAACAAA
GCACGTTTTACCACCTTGAACCCCAGCGCCTCCGTTTCCGACTGGGTGATTGACACAGTATCGACGCTTGGCAGTGGGTG
GTGTCCGCCCGGCCTGATCTCCGTCGGCATCGGTGGTAGCGCTGAAAAGGCGATGCTGCTGGCCAAGGAGGCCATGAACA
AGCCCATCGACATGTCGGAACTAATCGCCAGGGGGCCGTCAAGCGCGGAGGAGGCATTGCGGATTGAGCTTTATGAGCGG
ATTAACGCGCTGGGCATCGGCGCCCAAGGCCTCGGTGGCCTGACGACAGTCGTCGATGTCAAGGTTGCGACGTATCCTAC
CCATGCGGCGTCCAAGCCCGTGGCGCTCATTCCGCAATGCGCCGCCAACCGGCACCTGAAGTTTACCTTGGATGGCTCCG
GACCAATCAGGGTGCAGCCGCCCGATTTGTGCGAATGGCCCGACATTGGAACCGACGAATTGAGTCCAGCCGGCGTGCGA
AGAGTCAACCTCGATGCGTTGACCAAGGAAGAGACGGCATCGTGGCGCTGTGGTGAAACGCTCCTTCTGTCCGGAAAGAT
GCTGACAGGCCGTGACGCCGCCCACAAGCGAATGGTCGAGCTGATCGACGCCGGCAAACCGCTTCCGTTCGATCTGCAGG
GACGCGTGATATACTACGTTGGACCCGTCCGCGCCGTGCGAGATGAGGTCGTCGGACCGGCTGGCCCAACAACCTCCAGC
CGCTTGGACGATTTTACGGACAAGGTGCTCGCCGAAACCGGGCTTTTCGCGATGGTGGGCAAAGCGGAGAGGGGACGAGC
GGCCATCGAGTCGATCGTAAGACATAAAACGCCGTATCTCATCGCAGTGGGTGGGGCTGCCTACCTGATATCAAAATCAA
TTAAGTCAGCGCGGCTCATTGCCTTCGAAGACCTGGGTATGGAAGCAGTCTATGAATTCGAGGTGCAGGATATGCCCGTG
ATCATGGCTGTCGATGTTGATGGAAACTCCATTCACAATTCAGGGCCTCTTGAGTGGCGCAAGCGCATGGCGGCCGACAG
CATCGCACGCAACATCGGCGTTTGA

Upstream 100 bases:

>100_bases
CAAACAGAGCGGCTATGGATATGAAGGAGGCGAGCAGGGAGTGCGGAGCTTCCAGATCTTGAAAATGGTCAACGGCCTCA
GCTCATTTGGATGAAATCCG

Downstream 100 bases:

>100_bases
GCCTTCCGACTAAGCTCGGTTGGTGGGCGTCAATTCCGTTGTTGACAACGGCTAAGAGCAGAAGGTCATGAAGTCGAAAC
CCAATCCTGCGCGCCTGGAC

Product: fumarate hydratase, class I

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MGNRTRTIAGKDITRSVADALQYISYYHPPDYIRSLSQAYAREQSPAAKNAIGQILLNSRMAAFGRRPICQDTGLVVVFA
KVGMETRIKSTISFADLVNEGVRQAYLDPDNPLRASIVADPLAGRVNTRDNTPAVVHVDLVQGNQIEITIAAKGGGSENK
ARFTTLNPSASVSDWVIDTVSTLGSGWCPPGLISVGIGGSAEKAMLLAKEAMNKPIDMSELIARGPSSAEEALRIELYER
INALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHLKFTLDGSGPIRVQPPDLCEWPDIGTDELSPAGVR
RVNLDALTKEETASWRCGETLLLSGKMLTGRDAAHKRMVELIDAGKPLPFDLQGRVIYYVGPVRAVRDEVVGPAGPTTSS
RLDDFTDKVLAETGLFAMVGKAERGRAAIESIVRHKTPYLIAVGGAAYLISKSIKSARLIAFEDLGMEAVYEFEVQDMPV
IMAVDVDGNSIHNSGPLEWRKRMAADSIARNIGV

Sequences:

>Translated_514_residues
MGNRTRTIAGKDITRSVADALQYISYYHPPDYIRSLSQAYAREQSPAAKNAIGQILLNSRMAAFGRRPICQDTGLVVVFA
KVGMETRIKSTISFADLVNEGVRQAYLDPDNPLRASIVADPLAGRVNTRDNTPAVVHVDLVQGNQIEITIAAKGGGSENK
ARFTTLNPSASVSDWVIDTVSTLGSGWCPPGLISVGIGGSAEKAMLLAKEAMNKPIDMSELIARGPSSAEEALRIELYER
INALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHLKFTLDGSGPIRVQPPDLCEWPDIGTDELSPAGVR
RVNLDALTKEETASWRCGETLLLSGKMLTGRDAAHKRMVELIDAGKPLPFDLQGRVIYYVGPVRAVRDEVVGPAGPTTSS
RLDDFTDKVLAETGLFAMVGKAERGRAAIESIVRHKTPYLIAVGGAAYLISKSIKSARLIAFEDLGMEAVYEFEVQDMPV
IMAVDVDGNSIHNSGPLEWRKRMAADSIARNIGV
>Mature_513_residues
GNRTRTIAGKDITRSVADALQYISYYHPPDYIRSLSQAYAREQSPAAKNAIGQILLNSRMAAFGRRPICQDTGLVVVFAK
VGMETRIKSTISFADLVNEGVRQAYLDPDNPLRASIVADPLAGRVNTRDNTPAVVHVDLVQGNQIEITIAAKGGGSENKA
RFTTLNPSASVSDWVIDTVSTLGSGWCPPGLISVGIGGSAEKAMLLAKEAMNKPIDMSELIARGPSSAEEALRIELYERI
NALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHLKFTLDGSGPIRVQPPDLCEWPDIGTDELSPAGVRR
VNLDALTKEETASWRCGETLLLSGKMLTGRDAAHKRMVELIDAGKPLPFDLQGRVIYYVGPVRAVRDEVVGPAGPTTSSR
LDDFTDKVLAETGLFAMVGKAERGRAAIESIVRHKTPYLIAVGGAAYLISKSIKSARLIAFEDLGMEAVYEFEVQDMPVI
MAVDVDGNSIHNSGPLEWRKRMAADSIARNIGV

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=452, Percent_Identity=33.1858407079646, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1787897, Length=454, Percent_Identity=32.3788546255507, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1789442, Length=291, Percent_Identity=31.2714776632302, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789443, Length=178, Percent_Identity=30.3370786516854, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 55142; Mature: 55011

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNRTRTIAGKDITRSVADALQYISYYHPPDYIRSLSQAYAREQSPAAKNAIGQILLNSR
CCCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MAAFGRRPICQDTGLVVVFAKVGMETRIKSTISFADLVNEGVRQAYLDPDNPLRASIVAD
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PLAGRVNTRDNTPAVVHVDLVQGNQIEITIAAKGGGSENKARFTTLNPSASVSDWVIDTV
CCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
STLGSGWCPPGLISVGIGGSAEKAMLLAKEAMNKPIDMSELIARGPSSAEEALRIELYER
HHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
INALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHLKFTLDGSGPIRVQP
HHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCHHCCCCEEEEEECCCCCEEECC
PDLCEWPDIGTDELSPAGVRRVNLDALTKEETASWRCGETLLLSGKMLTGRDAAHKRMVE
CCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHCCCCCCCCEEEEECCEECCCHHHHHHHHH
LIDAGKPLPFDLQGRVIYYVGPVRAVRDEVVGPAGPTTSSRLDDFTDKVLAETGLFAMVG
HHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
KAERGRAAIESIVRHKTPYLIAVGGAAYLISKSIKSARLIAFEDLGMEAVYEFEVQDMPV
CCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCE
IMAVDVDGNSIHNSGPLEWRKRMAADSIARNIGV
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNRTRTIAGKDITRSVADALQYISYYHPPDYIRSLSQAYAREQSPAAKNAIGQILLNSR
CCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MAAFGRRPICQDTGLVVVFAKVGMETRIKSTISFADLVNEGVRQAYLDPDNPLRASIVAD
HHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
PLAGRVNTRDNTPAVVHVDLVQGNQIEITIAAKGGGSENKARFTTLNPSASVSDWVIDTV
CCCCCCCCCCCCCEEEEEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHH
STLGSGWCPPGLISVGIGGSAEKAMLLAKEAMNKPIDMSELIARGPSSAEEALRIELYER
HHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHH
INALGIGAQGLGGLTTVVDVKVATYPTHAASKPVALIPQCAANRHLKFTLDGSGPIRVQP
HHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEECCHHCCCCEEEEEECCCCCEEECC
PDLCEWPDIGTDELSPAGVRRVNLDALTKEETASWRCGETLLLSGKMLTGRDAAHKRMVE
CCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHCCCCCCCCEEEEECCEECCCHHHHHHHHH
LIDAGKPLPFDLQGRVIYYVGPVRAVRDEVVGPAGPTTSSRLDDFTDKVLAETGLFAMVG
HHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEC
KAERGRAAIESIVRHKTPYLIAVGGAAYLISKSIKSARLIAFEDLGMEAVYEFEVQDMPV
CCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCE
IMAVDVDGNSIHNSGPLEWRKRMAADSIARNIGV
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]