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The map label for this gene is purT [C]

Identifier: 13475061

GI number: 13475061

Start: 4900276

End: 4901505

Strand: Reverse

Name: purT [C]

Synonym: mll6062

Alternate gene names: 13475061

Gene position: 4901505-4900276 (Counterclockwise)

Preceding gene: 13475062

Following gene: 13475058

Centisome position: 69.66

GC content: 56.91

Gene sequence:

>1230_bases
ATGACAAGAAAAACGCTAATCCTGCTTGAAGGCCATCCATGGCATAATGGTCTGCTATACGTCCAAGCGGCCCAGCGTCT
TGGTCTTCATCCAATCACCCTGTCGGCTGATCCAGCTCAGTACGACTATCTTGCGACGGAAGGCATTGAGGCAATACGTG
TCGACACAGGCAATCTCGATGCACTGATCCGCGAATGTTCCCGGCTCCGTGCGACCTACGACATTGCTGGGATTACGTGC
GCCAGAGAGGCGTTCTATGCGACAGTTGGCAAGCTCTGCCGGCATTTCGATCTACCGGGACCGAACCCCGCATCGATTGA
ACGATGCTGCGATAAATTCACTCAACGTCAGCTCCTCGCGGAGGCCGGCGTTCCAATACCGGCTTATCGCATGGCGGCGA
ATGCGGCGGAGGTGGAAAGCGCTTCCGAGGAGATCGGCCTGCCGGTGATTGTTAAGCCAGCCGTGGGCAGCGGCAGCAGG
GGCGTCCGATTGTGCCGCAACGTCGACGAGTTGGCTGAACATACGACCTATCTGTTGGGCGAGGAGCACATATGGCGGTC
TTCGCCGAGGATACTTGTCGAAGAATTCGCACAAGGCCCGCTTTATGGCGCTCAGATAATGGGAAATGAGGTCATAGGGA
TTGAGGCCGCTGGCTTCGGCCCCCCCCCGCATTTCGTCTATCGTGAGTTCACCTTTCCGGCCCTGCTCACTGATGACGAT
CATGAGCGTATCACCAATGTTTCGCTGAGCTGTTTGCGAGCTCTCGGCCTTGGCTGGGGGCCAACGGGCATTGAATTCCG
GTGGACGAAGCGTGGCCCCGTCGTCATTGAAGTCAATCCGCGACTTGCTGGCATGCCCGATCCTCAACTGGTTCAGCTGG
CTTACGGTGTCGATCTCATCACCGAGCACATCAAGCTTGTAATCGGCGAGGAATGGAATTTGCGCAGAAGGCATTCGCAA
GTTGCGGCCGCGCGGTTCCTGGTTCCTGAACGCGATGGCACCCTGGATTGGATCACTGGCGACAGTCAGGCCGCTGCTGT
ATCAGGCGTCGCCGAGGTCAAATTCTATGTTGAACCCAACACGCTGATCGTCAGGAAAGGCGATCTCCGAGACTGGATCG
GATATGTCATTGCCGCTTCCCCCAGCCGTGCTCGGACCGAGGCGATACTTCAGCGTGCCGTAGACTTAATCGATTGGTCG
ATTACACCATTTTCGACCCCTGGCGAATAG

Upstream 100 bases:

>100_bases
ATGGTACGTTCACCACAAGGTCTGAGGTCAGGCAGGGCTAAAAAGCTGATGCCGATTCACCTCGACCGCCGCACGACAGG
CAACGTCGGAGTCCAAGTAA

Downstream 100 bases:

>100_bases
GAGCGACCTGCGGCCCCCTCAAGCACTCAAGCACTTGTCCCGGCGCACCCTGTTGCGGGTCACCACCGCCCAGCCCATCG
CACCGTGAATTTGAAACGCA

Product: hypothetical protein

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRKTLILLEGHPWHNGLLYVQAAQRLGLHPITLSADPAQYDYLATEGIEAIRVDTGNLDALIRECSRLRATYDIAGITC
AREAFYATVGKLCRHFDLPGPNPASIERCCDKFTQRQLLAEAGVPIPAYRMAANAAEVESASEEIGLPVIVKPAVGSGSR
GVRLCRNVDELAEHTTYLLGEEHIWRSSPRILVEEFAQGPLYGAQIMGNEVIGIEAAGFGPPPHFVYREFTFPALLTDDD
HERITNVSLSCLRALGLGWGPTGIEFRWTKRGPVVIEVNPRLAGMPDPQLVQLAYGVDLITEHIKLVIGEEWNLRRRHSQ
VAAARFLVPERDGTLDWITGDSQAAAVSGVAEVKFYVEPNTLIVRKGDLRDWIGYVIAASPSRARTEAILQRAVDLIDWS
ITPFSTPGE

Sequences:

>Translated_409_residues
MTRKTLILLEGHPWHNGLLYVQAAQRLGLHPITLSADPAQYDYLATEGIEAIRVDTGNLDALIRECSRLRATYDIAGITC
AREAFYATVGKLCRHFDLPGPNPASIERCCDKFTQRQLLAEAGVPIPAYRMAANAAEVESASEEIGLPVIVKPAVGSGSR
GVRLCRNVDELAEHTTYLLGEEHIWRSSPRILVEEFAQGPLYGAQIMGNEVIGIEAAGFGPPPHFVYREFTFPALLTDDD
HERITNVSLSCLRALGLGWGPTGIEFRWTKRGPVVIEVNPRLAGMPDPQLVQLAYGVDLITEHIKLVIGEEWNLRRRHSQ
VAAARFLVPERDGTLDWITGDSQAAAVSGVAEVKFYVEPNTLIVRKGDLRDWIGYVIAASPSRARTEAILQRAVDLIDWS
ITPFSTPGE
>Mature_408_residues
TRKTLILLEGHPWHNGLLYVQAAQRLGLHPITLSADPAQYDYLATEGIEAIRVDTGNLDALIRECSRLRATYDIAGITCA
REAFYATVGKLCRHFDLPGPNPASIERCCDKFTQRQLLAEAGVPIPAYRMAANAAEVESASEEIGLPVIVKPAVGSGSRG
VRLCRNVDELAEHTTYLLGEEHIWRSSPRILVEEFAQGPLYGAQIMGNEVIGIEAAGFGPPPHFVYREFTFPALLTDDDH
ERITNVSLSCLRALGLGWGPTGIEFRWTKRGPVVIEVNPRLAGMPDPQLVQLAYGVDLITEHIKLVIGEEWNLRRRHSQV
AAARFLVPERDGTLDWITGDSQAAAVSGVAEVKFYVEPNTLIVRKGDLRDWIGYVIAASPSRARTEAILQRAVDLIDWSI
TPFSTPGE

Specific function: Catalyzes Two Reactions:The First One Is The Production Of Beta-Formyl Glycinamide Ribonucleotide (Gar) From Formate, ATP And Beta Gar; The Second, A Side Reaction, Is The Production Of Acetyl Phosphate And ADP From Acetate And ATP. [C]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI148839342, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261245046, Length=238, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479 [H]

Pfam domain/function: PF02786 CPSase_L_D2 [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 45067; Mature: 44936

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50975 ATP_GRASP ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKTLILLEGHPWHNGLLYVQAAQRLGLHPITLSADPAQYDYLATEGIEAIRVDTGNLD
CCCEEEEEEECCCCCCCEEEEEEHHHCCCCEEEECCCCCCCCHHHHCCCEEEEECCCCHH
ALIRECSRLRATYDIAGITCAREAFYATVGKLCRHFDLPGPNPASIERCCDKFTQRQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EAGVPIPAYRMAANAAEVESASEEIGLPVIVKPAVGSGSRGVRLCRNVDELAEHTTYLLG
HCCCCCCHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHC
EEHIWRSSPRILVEEFAQGPLYGAQIMGNEVIGIEAAGFGPPPHFVYREFTFPALLTDDD
CHHHHCCCCHHHHHHHHCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCEEECCCC
HERITNVSLSCLRALGLGWGPTGIEFRWTKRGPVVIEVNPRLAGMPDPQLVQLAYGVDLI
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
TEHIKLVIGEEWNLRRRHSQVAAARFLVPERDGTLDWITGDSQAAAVSGVAEVKFYVEPN
HHHHHEEECCCCCHHHHHHHHHHHHEECCCCCCCEEEEECCCCHHHHCCEEEEEEEECCC
TLIVRKGDLRDWIGYVIAASPSRARTEAILQRAVDLIDWSITPFSTPGE
EEEEECCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TRKTLILLEGHPWHNGLLYVQAAQRLGLHPITLSADPAQYDYLATEGIEAIRVDTGNLD
CCEEEEEEECCCCCCCEEEEEEHHHCCCCEEEECCCCCCCCHHHHCCCEEEEECCCCHH
ALIRECSRLRATYDIAGITCAREAFYATVGKLCRHFDLPGPNPASIERCCDKFTQRQLLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EAGVPIPAYRMAANAAEVESASEEIGLPVIVKPAVGSGSRGVRLCRNVDELAEHTTYLLG
HCCCCCCHHHHHCCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHC
EEHIWRSSPRILVEEFAQGPLYGAQIMGNEVIGIEAAGFGPPPHFVYREFTFPALLTDDD
CHHHHCCCCHHHHHHHHCCCCCCCEECCCEEEEEEECCCCCCCCCEEEEECCCEEECCCC
HERITNVSLSCLRALGLGWGPTGIEFRWTKRGPVVIEVNPRLAGMPDPQLVQLAYGVDLI
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
TEHIKLVIGEEWNLRRRHSQVAAARFLVPERDGTLDWITGDSQAAAVSGVAEVKFYVEPN
HHHHHEEECCCCCHHHHHHHHHHHHEECCCCCCCEEEEECCCCHHHHCCEEEEEEEECCC
TLIVRKGDLRDWIGYVIAASPSRARTEAILQRAVDLIDWSITPFSTPGE
EEEEECCCHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]