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The map label for this gene is dapE [H]

Identifier: 13475027

GI number: 13475027

Start: 4865426

End: 4866886

Strand: Reverse

Name: dapE [H]

Synonym: mll6018

Alternate gene names: 13475027

Gene position: 4866886-4865426 (Counterclockwise)

Preceding gene: 13475028

Following gene: 13475026

Centisome position: 69.17

GC content: 55.78

Gene sequence:

>1461_bases
ATGCACACCGATGCAATTGTACAGTATCTTCACAACCACCAAAACGACATTGTAGATAGACTTTGCGCTTTCTTGCGCCT
GCCAAGTGTAAGCACGGACCCAGCTTTCACAGGAGGGATGCGCGACGCGCAAACGTTTCTGGTGACCTGGCTCAAAAGCA
TGGGCTTGAGCGATGTCCAGCTTCTCGATGGCGGAGGCCATCCCGCTGTCTATGGTGCTTGGAATGGTGCGCCGGGAAAA
CCTACGTTGCTCATCTATGGACACTATGATGTACAGCCTCCCGACCCGCTGGACGCTTGGGTGACTCCACCTTTCGAACC
AACGATCCGAGACGGACGGCTCTATGCGCGCGGTGCATCTGACGACAAAGGCTCGACTGCAATCGCGCTAGAAACTATCG
CTGCTTTTCTCAACGTCCGAGGCGCCTGCCCAGTGAACGTAAAAGTCTTTTTGGAAGGGGAAGAGGAAATCAATAGTCCC
AGTTTGCGTGCGATTGTGGAACTGTACAGCGACTTGCTGCAGGCTGATGGAATGATATCCACTGATGACTCGCGAGTGCA
TTCCGAAATCCCGACGATTAATGTCGGCTTGCGTGGAATTGTGGAGTTTGAGATTTCAATCAAAACCGCCGACAAGGACC
TTCATTCTGGTCTCTACGGTGGCGCGGTTCGGAACGCCGCACACGAGATGGCGAGGATCATCGCTTCGTTGCATGACGAG
AGTGGCGCGATAGCGGTCCCGGGATTGGTGCCGCCCATATCTTCCGTCCCCGTCCACGTACGAGCTGAGACGGCCAGATT
TCCCTTCGACGAGGTCACTTTCGTCAAAGACGTTGGGGCGTGCGCCCAAGGAGAACCCGGCTTCTCGGTACGTGAACAGC
TGACGCTTCGACCGGCTTTGGACATAAACGGAATGTGGGGAGGTTACATCGGATCCGGCAGCAAAACAGTAATCCCAAGA
ACTGCCAACGCTAAGCTGTCCTTGAGAACCGTGCCGGGACAGGACCCCGATCAAGTTTCAAACGCGCTAAAGGCTCATCT
GCGTGCTGTTTGCCCGTCGGACGTTGAGCTCTCGATCGATGACCCGGGAACCGGGTGTCGCGCCTTCGACCTGCCTACCG
GACACCCCCTTCTGCTTGCCGCTAAAAGAGTCTTGTCCGAGGCGCACGGCCAAGAGTCAGTGCTTGTGCGTCTGGGCGCG
TCCATACCCGTCACCGCGGTATTCGAAGAGCTGCTCGGAATTCAGACGCTTATGTTCGGCTTTGCCCTCTATGATGAAGA
TATTCATGCACCCAACGAATTCTTTCGGTTGGCCTCGTTAGCGGAAGGCCTATCTGCGTGGCCAAGGCTACTAGAGCAAG
TCGGCGAGCTCAGCGTCGATGAGTTTCGATCGATGGCTGCGGCGGCCGCATCCGATACCCAAGTGAAACATGCGGAGCAA
GTGAGGGCGTTGATGACTTGA

Upstream 100 bases:

>100_bases
TCGACGCGACCGCGAAATCGGTCGATACGACTTACCGTGCGCTAAAAGCCGAGGCTGCGATGTGACCATGGCCGCACATT
CATGAGTAGGAGTAGAAGCG

Downstream 100 bases:

>100_bases
ACCATCCATTGGAGAGCCTGGCTTGGCATGTCTCTAGGATTAAGCGATCGGTCGGCCGAGAATCCCTTCACCACCTACTC
GTGTGGAGAAAAGCTTGACC

Product: hypothetical protein

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MHTDAIVQYLHNHQNDIVDRLCAFLRLPSVSTDPAFTGGMRDAQTFLVTWLKSMGLSDVQLLDGGGHPAVYGAWNGAPGK
PTLLIYGHYDVQPPDPLDAWVTPPFEPTIRDGRLYARGASDDKGSTAIALETIAAFLNVRGACPVNVKVFLEGEEEINSP
SLRAIVELYSDLLQADGMISTDDSRVHSEIPTINVGLRGIVEFEISIKTADKDLHSGLYGGAVRNAAHEMARIIASLHDE
SGAIAVPGLVPPISSVPVHVRAETARFPFDEVTFVKDVGACAQGEPGFSVREQLTLRPALDINGMWGGYIGSGSKTVIPR
TANAKLSLRTVPGQDPDQVSNALKAHLRAVCPSDVELSIDDPGTGCRAFDLPTGHPLLLAAKRVLSEAHGQESVLVRLGA
SIPVTAVFEELLGIQTLMFGFALYDEDIHAPNEFFRLASLAEGLSAWPRLLEQVGELSVDEFRSMAAAAASDTQVKHAEQ
VRALMT

Sequences:

>Translated_486_residues
MHTDAIVQYLHNHQNDIVDRLCAFLRLPSVSTDPAFTGGMRDAQTFLVTWLKSMGLSDVQLLDGGGHPAVYGAWNGAPGK
PTLLIYGHYDVQPPDPLDAWVTPPFEPTIRDGRLYARGASDDKGSTAIALETIAAFLNVRGACPVNVKVFLEGEEEINSP
SLRAIVELYSDLLQADGMISTDDSRVHSEIPTINVGLRGIVEFEISIKTADKDLHSGLYGGAVRNAAHEMARIIASLHDE
SGAIAVPGLVPPISSVPVHVRAETARFPFDEVTFVKDVGACAQGEPGFSVREQLTLRPALDINGMWGGYIGSGSKTVIPR
TANAKLSLRTVPGQDPDQVSNALKAHLRAVCPSDVELSIDDPGTGCRAFDLPTGHPLLLAAKRVLSEAHGQESVLVRLGA
SIPVTAVFEELLGIQTLMFGFALYDEDIHAPNEFFRLASLAEGLSAWPRLLEQVGELSVDEFRSMAAAAASDTQVKHAEQ
VRALMT
>Mature_486_residues
MHTDAIVQYLHNHQNDIVDRLCAFLRLPSVSTDPAFTGGMRDAQTFLVTWLKSMGLSDVQLLDGGGHPAVYGAWNGAPGK
PTLLIYGHYDVQPPDPLDAWVTPPFEPTIRDGRLYARGASDDKGSTAIALETIAAFLNVRGACPVNVKVFLEGEEEINSP
SLRAIVELYSDLLQADGMISTDDSRVHSEIPTINVGLRGIVEFEISIKTADKDLHSGLYGGAVRNAAHEMARIIASLHDE
SGAIAVPGLVPPISSVPVHVRAETARFPFDEVTFVKDVGACAQGEPGFSVREQLTLRPALDINGMWGGYIGSGSKTVIPR
TANAKLSLRTVPGQDPDQVSNALKAHLRAVCPSDVELSIDDPGTGCRAFDLPTGHPLLLAAKRVLSEAHGQESVLVRLGA
SIPVTAVFEELLGIQTLMFGFALYDEDIHAPNEFFRLASLAEGLSAWPRLLEQVGELSVDEFRSMAAAAASDTQVKHAEQ
VRALMT

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Homo sapiens, GI271398239, Length=475, Percent_Identity=31.5789473684211, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI21071039, Length=474, Percent_Identity=27.2151898734177, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI271398277, Length=325, Percent_Identity=32, Blast_Score=150, Evalue=3e-36,
Organism=Escherichia coli, GI1788816, Length=111, Percent_Identity=39.6396396396396, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17563146, Length=473, Percent_Identity=30.4439746300211, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI193211059, Length=476, Percent_Identity=28.1512605042017, Blast_Score=172, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321159, Length=485, Percent_Identity=25.5670103092783, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319758, Length=439, Percent_Identity=28.0182232346241, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24585880, Length=469, Percent_Identity=29.4243070362473, Blast_Score=197, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 52208; Mature: 52208

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTDAIVQYLHNHQNDIVDRLCAFLRLPSVSTDPAFTGGMRDAQTFLVTWLKSMGLSDVQ
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLDGGGHPAVYGAWNGAPGKPTLLIYGHYDVQPPDPLDAWVTPPFEPTIRDGRLYARGAS
EECCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DDKGSTAIALETIAAFLNVRGACPVNVKVFLEGEEEINSPSLRAIVELYSDLLQADGMIS
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCCC
TDDSRVHSEIPTINVGLRGIVEFEISIKTADKDLHSGLYGGAVRNAAHEMARIIASLHDE
CCCHHHHHCCCEEEECCEEEEEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
SGAIAVPGLVPPISSVPVHVRAETARFPFDEVTFVKDVGACAQGEPGFSVREQLTLRPAL
CCCEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHEEECCCC
DINGMWGGYIGSGSKTVIPRTANAKLSLRTVPGQDPDQVSNALKAHLRAVCPSDVELSID
CCCCCCCCEECCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
DPGTGCRAFDLPTGHPLLLAAKRVLSEAHGQESVLVRLGASIPVTAVFEELLGIQTLMFG
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
FALYDEDIHAPNEFFRLASLAEGLSAWPRLLEQVGELSVDEFRSMAAAAASDTQVKHAEQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
VRALMT
HHHHCC
>Mature Secondary Structure
MHTDAIVQYLHNHQNDIVDRLCAFLRLPSVSTDPAFTGGMRDAQTFLVTWLKSMGLSDVQ
CCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LLDGGGHPAVYGAWNGAPGKPTLLIYGHYDVQPPDPLDAWVTPPFEPTIRDGRLYARGAS
EECCCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
DDKGSTAIALETIAAFLNVRGACPVNVKVFLEGEEEINSPSLRAIVELYSDLLQADGMIS
CCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCCC
TDDSRVHSEIPTINVGLRGIVEFEISIKTADKDLHSGLYGGAVRNAAHEMARIIASLHDE
CCCHHHHHCCCEEEECCEEEEEEEEEEEECCHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
SGAIAVPGLVPPISSVPVHVRAETARFPFDEVTFVKDVGACAQGEPGFSVREQLTLRPAL
CCCEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHEEECCCC
DINGMWGGYIGSGSKTVIPRTANAKLSLRTVPGQDPDQVSNALKAHLRAVCPSDVELSID
CCCCCCCCEECCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
DPGTGCRAFDLPTGHPLLLAAKRVLSEAHGQESVLVRLGASIPVTAVFEELLGIQTLMFG
CCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
FALYDEDIHAPNEFFRLASLAEGLSAWPRLLEQVGELSVDEFRSMAAAAASDTQVKHAEQ
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
VRALMT
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA