Click here to switch to the map view.

The map label for this gene is noeL [H]

Identifier: 13474933

GI number: 13474933

Start: 4788062

End: 4788577

Strand: Reverse

Name: noeL [H]

Synonym: mll5922

Alternate gene names: 13474933

Gene position: 4788577-4788062 (Counterclockwise)

Preceding gene: 13474943

Following gene: 13474932

Centisome position: 68.06

GC content: 59.3

Gene sequence:

>516_bases
GTGGCGAAGCTCTACGCCTACTGGACCACCATGAACTACCGCGAGGTATACGGGCTCTATGCAAGCAACGGCATCCTGTT
CAATCACGAAGGGCCTACGCGCGGCGAAACTTTCATCACGCGCAAGGTGACGCGTGCGGTGGCTCGATCATGGACCGGCA
AACAGGACTGTCTCTATCTCGGAAACCTGGAAGCAAAGCGCGACTGGGGCTCGGCGCGCGCCTATGTCGAGGGCATGTGG
CGCATCTTGCAGCATCAACCTGACGATTTCGTGCTGGCAACCGGCGAGACACACACGGTCCGCACCTTTGTCGGGGCTGC
CTTCAGGCGAGTCGAAAAGGAGATCGTCTGGGAGGGCGAAGGCGTTGACGAGGTGGGCCGTGAACGCATGAGTAATCAAG
CGCTGATCCGGATCGACAAACGCTGTGTCGGGCCAATTGAGGTTGACCTGCTGATCGGCGATGCCTCCAAGGCAGCCGAC
CAAACTCGGTTGGAAGCCAAGGACCACTTTCGATGA

Upstream 100 bases:

>100_bases
ACGGATGCGGCAAACCTGATACGTCTCCGGCAAGAAACGCAGCCCGACGAAATTCCCGCAGCAGGAGAGCACTCCGTTTT
ATCCACGCTCGACCTATGCG

Downstream 100 bases:

>100_bases
GCTCATCTCGAAAATGGTCGAGGCCGACTGCCGCGCCTACGGGATCGTGTTAGCGTGACGTGAAAACACGATGACTTCGC
GGGCATGTGTATCTTGCCGT

Product: GDP-D-mannose dehydratase(nodulation protein NoeL)

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAKLYAYWTTMNYREVYGLYASNGILFNHEGPTRGETFITRKVTRAVARSWTGKQDCLYLGNLEAKRDWGSARAYVEGMW
RILQHQPDDFVLATGETHTVRTFVGAAFRRVEKEIVWEGEGVDEVGRERMSNQALIRIDKRCVGPIEVDLLIGDASKAAD
QTRLEAKDHFR

Sequences:

>Translated_171_residues
MAKLYAYWTTMNYREVYGLYASNGILFNHEGPTRGETFITRKVTRAVARSWTGKQDCLYLGNLEAKRDWGSARAYVEGMW
RILQHQPDDFVLATGETHTVRTFVGAAFRRVEKEIVWEGEGVDEVGRERMSNQALIRIDKRCVGPIEVDLLIGDASKAAD
QTRLEAKDHFR
>Mature_170_residues
AKLYAYWTTMNYREVYGLYASNGILFNHEGPTRGETFITRKVTRAVARSWTGKQDCLYLGNLEAKRDWGSARAYVEGMWR
ILQHQPDDFVLATGETHTVRTFVGAAFRRVEKEIVWEGEGVDEVGRERMSNQALIRIDKRCVGPIEVDLLIGDASKAADQ
TRLEAKDHFR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=161, Percent_Identity=52.7950310559006, Blast_Score=187, Evalue=5e-48,
Organism=Escherichia coli, GI1788366, Length=172, Percent_Identity=48.8372093023256, Blast_Score=169, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI133901786, Length=168, Percent_Identity=52.3809523809524, Blast_Score=185, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17539424, Length=168, Percent_Identity=52.3809523809524, Blast_Score=185, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI133901788, Length=168, Percent_Identity=52.3809523809524, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17539422, Length=168, Percent_Identity=52.3809523809524, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI133901790, Length=168, Percent_Identity=52.3809523809524, Blast_Score=184, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17507723, Length=159, Percent_Identity=52.2012578616352, Blast_Score=179, Evalue=8e-46,
Organism=Drosophila melanogaster, GI24158427, Length=158, Percent_Identity=56.3291139240506, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 19589; Mature: 19458

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLYAYWTTMNYREVYGLYASNGILFNHEGPTRGETFITRKVTRAVARSWTGKQDCLYL
CCEEEEEEEECCHHHHHEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
GNLEAKRDWGSARAYVEGMWRILQHQPDDFVLATGETHTVRTFVGAAFRRVEKEIVWEGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHEECCC
GVDEVGRERMSNQALIRIDKRCVGPIEVDLLIGDASKAADQTRLEAKDHFR
CHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHCHHCCCC
>Mature Secondary Structure 
AKLYAYWTTMNYREVYGLYASNGILFNHEGPTRGETFITRKVTRAVARSWTGKQDCLYL
CEEEEEEEECCHHHHHEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEE
GNLEAKRDWGSARAYVEGMWRILQHQPDDFVLATGETHTVRTFVGAAFRRVEKEIVWEGE
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHEECCC
GVDEVGRERMSNQALIRIDKRCVGPIEVDLLIGDASKAADQTRLEAKDHFR
CHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]