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The map label for this gene is pyrE

Identifier: 13474929

GI number: 13474929

Start: 4783453

End: 4784049

Strand: Reverse

Name: pyrE

Synonym: mll5916

Alternate gene names: 13474929

Gene position: 4784049-4783453 (Counterclockwise)

Preceding gene: 13474930

Following gene: 13474918

Centisome position: 67.99

GC content: 54.94

Gene sequence:

>597_bases
ATGGCGGCACTGGCGAAATTCAGACCAGTTGATTCCGGCCAACACGACAAGGACGAACTCCGCGAGATTGTCCGTGAGCG
CTCTTTTCGATTCGGACGCTACACGCTCTCGTCGGGCATAGAGAGCGACATCTATTTCAATATGAAGCCTACCATGATGG
TGGCGCGAGGCGCGCAACTTGCCGCTCTTGAATTCCTAAAAATAGTTGAGCAAGTGAAAGCAGAGTACGTCGGCGGTCTT
GAAATGGGAGCGGTCCCCGTAATCGGATCGATGGCGGCTGTCAGCTCGATGATGAACAAACCCATAAACACGATCTTCGT
TCGCAAAGTTCCAAAGGCCCATGGCACAAGGGACGTGATTGAAGGACTTGGGCCTAAAGAAGATCTCGAGGGAAAAGTGG
TCCTGATCGTTGACGACGTTGCCACAAGCGGCAAGTCGATATTGAAGGCGATTGAAGAGGTTCGCCGCGTCGGCGGCATT
GTGGGTGACGCTGCTTGCCTCGTGGACCGTGACGAGGGAGCTACGGCGCTGCTAGCCCAACATGGCGTCACGCTGCACTC
CGTACTGCATGCGAGCGAATTTGTGGAACGCCACTGA

Upstream 100 bases:

>100_bases
ATTAATTGACACACCCCGCGAACACTTGCATAAGCCACATAGCGGTAGTGACTAGGTGCATGCCGCAGCCAATTTTTTCC
CTACGGGCGAGGTCTGATCT

Downstream 100 bases:

>100_bases
CAATCTCATACAACGTCTTTCCGGCGACCTAGCCTTAGAGGACATTCCGCCGCTTTGCAGTGAGTGCAGGCGCCTCGCGA
GTAAGAGCGATTGCCCCTGC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT 1; OPRTase 1

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAALAKFRPVDSGQHDKDELREIVRERSFRFGRYTLSSGIESDIYFNMKPTMMVARGAQLAALEFLKIVEQVKAEYVGGL
EMGAVPVIGSMAAVSSMMNKPINTIFVRKVPKAHGTRDVIEGLGPKEDLEGKVVLIVDDVATSGKSILKAIEEVRRVGGI
VGDAACLVDRDEGATALLAQHGVTLHSVLHASEFVERH

Sequences:

>Translated_198_residues
MAALAKFRPVDSGQHDKDELREIVRERSFRFGRYTLSSGIESDIYFNMKPTMMVARGAQLAALEFLKIVEQVKAEYVGGL
EMGAVPVIGSMAAVSSMMNKPINTIFVRKVPKAHGTRDVIEGLGPKEDLEGKVVLIVDDVATSGKSILKAIEEVRRVGGI
VGDAACLVDRDEGATALLAQHGVTLHSVLHASEFVERH
>Mature_197_residues
AALAKFRPVDSGQHDKDELREIVRERSFRFGRYTLSSGIESDIYFNMKPTMMVARGAQLAALEFLKIVEQVKAEYVGGLE
MGAVPVIGSMAAVSSMMNKPINTIFVRKVPKAHGTRDVIEGLGPKEDLEGKVVLIVDDVATSGKSILKAIEEVRRVGGIV
GDAACLVDRDEGATALLAQHGVTLHSVLHASEFVERH

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=173, Percent_Identity=31.7919075144509, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17508631, Length=177, Percent_Identity=32.7683615819209, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17554964, Length=180, Percent_Identity=30.5555555555556, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17933654, Length=187, Percent_Identity=32.0855614973262, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE1_RHILO (Q98AN7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106499.1
- ProteinModelPortal:   Q98AN7
- GeneID:   1229158
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll5916
- NMPDR:   fig|266835.1.peg.4602
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 21501; Mature: 21370

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 108-108 BINDING 109-109 BINDING 112-112 BINDING 114-114 BINDING 142-142 BINDING 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALAKFRPVDSGQHDKDELREIVRERSFRFGRYTLSSGIESDIYFNMKPTMMVARGAQL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEECCCCHHHHHCCCHH
AALEFLKIVEQVKAEYVGGLEMGAVPVIGSMAAVSSMMNKPINTIFVRKVPKAHGTRDVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHEECCCCCCCCHHHH
EGLGPKEDLEGKVVLIVDDVATSGKSILKAIEEVRRVGGIVGDAACLVDRDEGATALLAQ
HCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHH
HGVTLHSVLHASEFVERH
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AALAKFRPVDSGQHDKDELREIVRERSFRFGRYTLSSGIESDIYFNMKPTMMVARGAQL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCEEECCCCHHHHHCCCHH
AALEFLKIVEQVKAEYVGGLEMGAVPVIGSMAAVSSMMNKPINTIFVRKVPKAHGTRDVI
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEHEECCCCCCCCHHHH
EGLGPKEDLEGKVVLIVDDVATSGKSILKAIEEVRRVGGIVGDAACLVDRDEGATALLAQ
HCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEECCCCCHHHHHH
HGVTLHSVLHASEFVERH
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968