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The map label for this gene is nusA [H]

Identifier: 13474627

GI number: 13474627

Start: 4454541

End: 4456136

Strand: Direct

Name: nusA [H]

Synonym: mlr5551

Alternate gene names: 13474627

Gene position: 4454541-4456136 (Clockwise)

Preceding gene: 13474626

Following gene: 13474628

Centisome position: 63.31

GC content: 63.22

Gene sequence:

>1596_bases
ATGGTTGTAAGCGCCAACAGACTTGAACTGCTGCAGATTGCCGACGCGGTCGCGCGTGAAAAGTCGATCGACAAGTCGAT
CGTCATCGCCGCCATGGCCGATGCGATCCAGAAGGCGGCGCGTTCGCGTTACGGCCAGGAAACCAACATCCGCGCCGACA
TCAACCCGAACACCGGCGAGATGAAGCTGCAGCGGCTGATGGAAGTGGTCGAAAAGGTCGACGACTATGCCACCCAGATC
GCCATTTCCTCGGCCCGCGAGCGCAATCCCGACGCCCAGCTCGGCGACTTCATTGCCGAACAGCTGCCGCCGATGGATTT
CGGCCGCATCGCCGCCCAGTCGGCAAAGCAGGTCATCGTGCAGAAGGTGCGCGAGGCCGAGCGCGACCGCCAGTATGACG
AATACAAGGACCGCATCGGCGAGATCGTCAACGGCACCGTCAAGCGTGTCGAATATGGCAATGTCATCGTCGATCTCGGC
CGTGGCGAGGCGATCATCCGCCGCGACGAGCTGATTCCGCGCGAGAACTACAAGTACGGCGACCGCGTCCGAGCCTATGT
CTATGACGTGCGCCGCGAGCAGCGCGGCCCGCAGATATTCCTGTCGCGTACCCATCCGCAGTTCATGGCCAAGCTGTTCA
CCATGGAAGTGCCGGAAATCTATGACGGCATCATCGAGATCAAGTCGGTCGCCCGCGACCCGGGCTCGCGCGCCAAGATC
GCCGTCATCTCGCGTGATAGTTCGATCGATCCGGTCGGCGCCTGCGTCGGTATGCGCGGCAGCCGTGTCCAGGCCGTCGT
CGGTGAATTGCAGGGCGAGAAGATCGACATCATTCCGTGGTCGCCCTCTGCCGCCTCCTTCATCGTCAATGCGCTGCAGC
CGGCGGAAGTCGCCAAGGTCGTGCTCGACGAGGACGCCGAGCGCATCGAAGTGGTGGTTCCCGACGATCAGTTGTCGCTC
GCCATCGGCCGCCGCGGCCAGAACGTGCGTCTCGCCTCGCAGCTCACCGGCTGGGATATCGACATCCTGACCGAGGCCGA
GGAATCCGAACGCCGCCAGAAGGAATTCGTCGAGCGCTCGGCGCTGTTCATGGAGGCCCTCGACGTCGACGAGATGGTCG
GCCAGGTGCTGGCCTCCGAAGGCTTCACCAGCGTCGAGGAAGTCGCCTATGTCGACGCCGGCGAAATCGCCTCGATCGAC
GGCTTCGACGACGACACGGCTTCCGAAATCCAGACCCGTGCCCGCGAATACCTCGAAAAGATCGAGGCCGAGCATGACGA
GAAGCGCAAGGCGCTGGGCGTCAAGGACGAGCTGCGCGAAATCCCCGGCATCACCACCGCCATGATGGTGACGCTCGGCG
AGGACGGCGTGAAGACGATCGAGGATTTCGCCGGCTATGCCGCCGACGACCTGACCGGCTGGAAGGAACGCAAGGACGGC
GAGACCAAGGTCTATCCAGGCGTCCTGGCCAATCATGGTGTTTCGCGCGCCGACGCCGAACAGATGGTGCTCAATGCCCG
TCTCAAGGCCGGCTGGATTTCCGAGGACGAGCTTGCGGCCGAAGAAGCATCGGCTGACGAAGCCGTTGGTGCGTGA

Upstream 100 bases:

>100_bases
CCCGAGGGTGCGGAAGCGGACGCCACGGAAGAACACGAACAGGAAAGTTGATTGAGCTGCCGGGTCCAAAGGTCCGGCAA
ACAGGCTCGGGAGAAAAAAC

Downstream 100 bases:

>100_bases
GGTGAAACCGCATCGCACACAACCCGCCCTTGGACGAGATGAACGATCGCACCTGCATCGTTACCCGCAAGCAGGCCGAA
CCGGATGAACTGATCCGGTT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MVVSANRLELLQIADAVAREKSIDKSIVIAAMADAIQKAARSRYGQETNIRADINPNTGEMKLQRLMEVVEKVDDYATQI
AISSARERNPDAQLGDFIAEQLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGEIVNGTVKRVEYGNVIVDLG
RGEAIIRRDELIPRENYKYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
AVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPSAASFIVNALQPAEVAKVVLDEDAERIEVVVPDDQLSL
AIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFVERSALFMEALDVDEMVGQVLASEGFTSVEEVAYVDAGEIASID
GFDDDTASEIQTRAREYLEKIEAEHDEKRKALGVKDELREIPGITTAMMVTLGEDGVKTIEDFAGYAADDLTGWKERKDG
ETKVYPGVLANHGVSRADAEQMVLNARLKAGWISEDELAAEEASADEAVGA

Sequences:

>Translated_531_residues
MVVSANRLELLQIADAVAREKSIDKSIVIAAMADAIQKAARSRYGQETNIRADINPNTGEMKLQRLMEVVEKVDDYATQI
AISSARERNPDAQLGDFIAEQLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGEIVNGTVKRVEYGNVIVDLG
RGEAIIRRDELIPRENYKYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
AVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPSAASFIVNALQPAEVAKVVLDEDAERIEVVVPDDQLSL
AIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFVERSALFMEALDVDEMVGQVLASEGFTSVEEVAYVDAGEIASID
GFDDDTASEIQTRAREYLEKIEAEHDEKRKALGVKDELREIPGITTAMMVTLGEDGVKTIEDFAGYAADDLTGWKERKDG
ETKVYPGVLANHGVSRADAEQMVLNARLKAGWISEDELAAEEASADEAVGA
>Mature_531_residues
MVVSANRLELLQIADAVAREKSIDKSIVIAAMADAIQKAARSRYGQETNIRADINPNTGEMKLQRLMEVVEKVDDYATQI
AISSARERNPDAQLGDFIAEQLPPMDFGRIAAQSAKQVIVQKVREAERDRQYDEYKDRIGEIVNGTVKRVEYGNVIVDLG
RGEAIIRRDELIPRENYKYGDRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
AVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPSAASFIVNALQPAEVAKVVLDEDAERIEVVVPDDQLSL
AIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFVERSALFMEALDVDEMVGQVLASEGFTSVEEVAYVDAGEIASID
GFDDDTASEIQTRAREYLEKIEAEHDEKRKALGVKDELREIPGITTAMMVTLGEDGVKTIEDFAGYAADDLTGWKERKDG
ETKVYPGVLANHGVSRADAEQMVLNARLKAGWISEDELAAEEASADEAVGA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=464, Percent_Identity=42.8879310344828, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 58836; Mature: 58836

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSANRLELLQIADAVAREKSIDKSIVIAAMADAIQKAARSRYGQETNIRADINPNTGE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
MKLQRLMEVVEKVDDYATQIAISSARERNPDAQLGDFIAEQLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKVREAERDRQYDEYKDRIGEIVNGTVKRVEYGNVIVDLGRGEAIIRRDELIPRENYKYG
HHHHHHHHHCHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCEEEEHHHCCCCCCCCCC
DRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEE
AVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPSAASFIVNALQPAEVAKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VLDEDAERIEVVVPDDQLSLAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFVERS
HHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ALFMEALDVDEMVGQVLASEGFTSVEEVAYVDAGEIASIDGFDDDTASEIQTRAREYLEK
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IEAEHDEKRKALGVKDELREIPGITTAMMVTLGEDGVKTIEDFAGYAADDLTGWKERKDG
HHHHHHHHHHHCCCHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCHHHCCCCHHCCCC
ETKVYPGVLANHGVSRADAEQMVLNARLKAGWISEDELAAEEASADEAVGA
CCEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MVVSANRLELLQIADAVAREKSIDKSIVIAAMADAIQKAARSRYGQETNIRADINPNTGE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHH
MKLQRLMEVVEKVDDYATQIAISSARERNPDAQLGDFIAEQLPPMDFGRIAAQSAKQVIV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKVREAERDRQYDEYKDRIGEIVNGTVKRVEYGNVIVDLGRGEAIIRRDELIPRENYKYG
HHHHHHHHHCHHHHHHHHHHHHHCCHHHEEEECCEEEECCCCCEEEEHHHCCCCCCCCCC
DRVRAYVYDVRREQRGPQIFLSRTHPQFMAKLFTMEVPEIYDGIIEIKSVARDPGSRAKI
HHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEE
AVISRDSSIDPVGACVGMRGSRVQAVVGELQGEKIDIIPWSPSAASFIVNALQPAEVAKV
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHH
VLDEDAERIEVVVPDDQLSLAIGRRGQNVRLASQLTGWDIDILTEAEESERRQKEFVERS
HHCCCCCEEEEEECCCCEEEEECCCCCCEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHH
ALFMEALDVDEMVGQVLASEGFTSVEEVAYVDAGEIASIDGFDDDTASEIQTRAREYLEK
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
IEAEHDEKRKALGVKDELREIPGITTAMMVTLGEDGVKTIEDFAGYAADDLTGWKERKDG
HHHHHHHHHHHCCCHHHHHHCCCCCCHHEEEECCCHHHHHHHHHCCCHHHCCCCHHCCCC
ETKVYPGVLANHGVSRADAEQMVLNARLKAGWISEDELAAEEASADEAVGA
CCEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]