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The map label for this gene is miaB

Identifier: 13474614

GI number: 13474614

Start: 4442510

End: 4443862

Strand: Direct

Name: miaB

Synonym: mlr5534

Alternate gene names: 13474614

Gene position: 4442510-4443862 (Clockwise)

Preceding gene: 13474613

Following gene: 13474615

Centisome position: 63.14

GC content: 64.82

Gene sequence:

>1353_bases
GTGCGCGCCACGGCCGCGAAAAAGGTCTTCATCAAGACCTATGGCTGCCAGATGAACGTCTATGATTCACAGCGCATGGG
CGATGCGCTGGCCGCCGACGGCTACACCGCGACCGATGCCATCGACGAGGCCGATCTGGTGCTGCTCAACACCTGCCATA
TCAGGGAGAAGGCGGCGGAAAAGGTCTATTCCGAGCTCGGCCGCATCCGTGACATGAAGGCGGAGCGCGCCATCGCCGGC
CGTGAACTGCTGATCGGCGTCGCCGGCTGCGTGGCGCAGGCCGAGGGCGCTGAGATCATCCGCCGTTCGCCGGCCGTCGA
CCTGGTCATCGGGCCGCAGACCTATCACCGTCTGCCCGACGTGCTGGCGCGGGTGCGCGGCGGCGAGAAGATCGTCGAGA
CCGACTATGCCATCGAAGACAAGTTCGACCATCTGCCGCAGCCCAAGCGCGCCGAGGTCATCAAGCGCGGCGTCACCGCC
TTCCTCACCGTGCAGGAGGGCTGCGACAAGTTCTGCACCTTCTGCGTCGTGCCCTATACAAGGGGCTCTGAAGTGTCGCG
GCCGGTGGCGCAGATCGTCGCCGAGGCCGAACGTCTGGCCGAAGCCGGCGTGCGCGAGGTGACGCTGCTTGGCCAGAACG
TCAATGCCTGGCACGGCCAAGGCGAGAACGGCGAGGAATGGGGCCTTGGCCGCCTGCTGTTCCGGCTGGCCGAAATCCCT
GGACTGGCGCGGCTGCGCTATACCACCAGCCACCCGCGCGACATGGACGACGAACTGATATCAGCCCACCGCGACCTGCC
GTCGTTGATGCCCTATCTGCATCTGCCGGTGCAATCGGGGTCCGACCGCATCTTGAAGGCGATGAACCGCAGGCACACGG
CCAGGGATTATCTGGCCCTGCTCGACCGCATCCGTGCCGCGCGGCCCGATATCGCTTTGTCCGGCGACTTCATCGTCGGC
TTCCCCGGCGAGACGGAGGCCGATTTCGAGGCGACCATGGAACTGGTGCGCCAGGTGAACTACGCCTCGGCCTTTTCGTT
CAAATATTCGCCGCGCCCCGGCACGCCGGGCGCCGAGATGCCTGATCATGTGCCCGAAACGCTCAAGGACGAGCGGCTGC
AGCGCCTGCAGGCACTGCTGTTGAAGCAGCAGCAGGGCTTCGGTTCGAGCCTGGTCGGCAGCACCATCGACACGCTGATC
GAGAAGCCCGGCCGGCAGGCCGGCCAGAAGGTTGGCCGCACACCTTGGCTGCAGCCGGTTATTGTTGATGAAAAGGCCGG
CGAAATCGGTGACATTATCCAGGTGCGAATCACGAAGACGGGCTACAATAGCCTGTTCGCCGAATTGGCCTGA

Upstream 100 bases:

>100_bases
AATCGTCTGTTTTTTGTCACGGAAAGGCGCTAGAAGCCGCCGGATGGACTTGAACACGATTGAAAGCGACGACATCGGCA
GCGTGGCCGGAGAGCCGGCA

Downstream 100 bases:

>100_bases
TGGTCTCGGCAGATGAGGAGAGACGGTTGAGCGCAGCAGAGCTGAAGAACCTGCCCCCGGTACCGGCATCTGGGGCTTCT
GACATGGCGCACATCGTTCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MRATAAKKVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAEKVYSELGRIRDMKAERAIAG
RELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPDVLARVRGGEKIVETDYAIEDKFDHLPQPKRAEVIKRGVTA
FLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIP
GLARLRYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLALLDRIRAARPDIALSGDFIVG
FPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEMPDHVPETLKDERLQRLQALLLKQQQGFGSSLVGSTIDTLI
EKPGRQAGQKVGRTPWLQPVIVDEKAGEIGDIIQVRITKTGYNSLFAELA

Sequences:

>Translated_450_residues
MRATAAKKVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAEKVYSELGRIRDMKAERAIAG
RELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPDVLARVRGGEKIVETDYAIEDKFDHLPQPKRAEVIKRGVTA
FLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIP
GLARLRYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLALLDRIRAARPDIALSGDFIVG
FPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEMPDHVPETLKDERLQRLQALLLKQQQGFGSSLVGSTIDTLI
EKPGRQAGQKVGRTPWLQPVIVDEKAGEIGDIIQVRITKTGYNSLFAELA
>Mature_450_residues
MRATAAKKVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAEKVYSELGRIRDMKAERAIAG
RELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPDVLARVRGGEKIVETDYAIEDKFDHLPQPKRAEVIKRGVTA
FLTVQEGCDKFCTFCVVPYTRGSEVSRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIP
GLARLRYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLALLDRIRAARPDIALSGDFIVG
FPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEMPDHVPETLKDERLQRLQALLLKQQQGFGSSLVGSTIDTLI
EKPGRQAGQKVGRTPWLQPVIVDEKAGEIGDIIQVRITKTGYNSLFAELA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=34.1052631578947, Blast_Score=278, Evalue=6e-75,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=34.1052631578947, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI93277076, Length=444, Percent_Identity=27.9279279279279, Blast_Score=177, Evalue=3e-44,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=49.6613995485327, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787057, Length=461, Percent_Identity=29.5010845986985, Blast_Score=194, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=33.7579617834395, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=370, Percent_Identity=28.1081081081081, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI21356207, Length=476, Percent_Identity=34.2436974789916, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922432, Length=458, Percent_Identity=29.2576419213974, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_RHILO (Q98BK3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_106183.1
- ProteinModelPortal:   Q98BK3
- SMR:   Q98BK3
- GeneID:   1228844
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5534
- NMPDR:   fig|266835.1.peg.4287
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49737; Mature: 49737

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATAAKKVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAE
CCCCHHHEEEEEEECCEEECCCHHHHCCHHHCCCCCHHCCCCCCCEEEEEHHHHHHHHHH
KVYSELGRIRDMKAERAIAGRELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHH
VLARVRGGEKIVETDYAIEDKFDHLPQPKRAEVIKRGVTAFLTVQEGCDKFCTFCVVPYT
HHHHHCCCCEEEECCCHHHHHHHCCCCCHHHHHHHCCHHHEEEHHHHHHHHHHHHEECCC
RGSEVSRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIP
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
GLARLRYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLAL
CCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
LDRIRAARPDIALSGDFIVGFPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEM
HHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
PDHVPETLKDERLQRLQALLLKQQQGFGSSLVGSTIDTLIEKPGRQAGQKVGRTPWLQPV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEE
IVDEKAGEIGDIIQVRITKTGYNSLFAELA
EECCCCCCCCCEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MRATAAKKVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAE
CCCCHHHEEEEEEECCEEECCCHHHHCCHHHCCCCCHHCCCCCCCEEEEEHHHHHHHHHH
KVYSELGRIRDMKAERAIAGRELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHHHHHHHH
VLARVRGGEKIVETDYAIEDKFDHLPQPKRAEVIKRGVTAFLTVQEGCDKFCTFCVVPYT
HHHHHCCCCEEEECCCHHHHHHHCCCCCHHHHHHHCCHHHEEEHHHHHHHHHHHHEECCC
RGSEVSRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIP
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
GLARLRYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLAL
CCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHH
LDRIRAARPDIALSGDFIVGFPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEM
HHHHHHCCCCEEECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
PDHVPETLKDERLQRLQALLLKQQQGFGSSLVGSTIDTLIEKPGRQAGQKVGRTPWLQPV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEE
IVDEKAGEIGDIIQVRITKTGYNSLFAELA
EECCCCCCCCCEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968