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The map label for this gene is mltB [H]

Identifier: 13474594

GI number: 13474594

Start: 4418948

End: 4420195

Strand: Direct

Name: mltB [H]

Synonym: mlr5511

Alternate gene names: 13474594

Gene position: 4418948-4420195 (Clockwise)

Preceding gene: 13474593

Following gene: 13474595

Centisome position: 62.8

GC content: 63.78

Gene sequence:

>1248_bases
ATGACCGCATTCCTTCGCACAATCGCCTTCGCCTTTATCGCGGCGCTTCTCGCCGTCCTCCTCGCGGCACCCGCCTCGGC
CGCAAAGATCGACGACCAGTTCCGCGCCTGGCTGCAGAACGATCTCTGGCCCGAGGCCAAAACCAAAGGCATTTCCAAGC
AGACTTTCGATGCCGCCTTCGATGGCATAAAGCCCAATCTGAAGCTGCCCGACCTTGTCATGCCCGGCGAGAAGCCGAAG
ACGCCGCAGAAGCAGCATCAGGCCGAGTTCGGCTCGCCCGGCGCCTATTTCGCCGAGAAGACGGTTCGTGCGGTGACGTC
GGGCGGCCGCACGCGCGAAGCCGCCAACGCCAAGACGCTTGCCGCGATCGAAAAGCGCTATGGCGTGCCCGGCGAGATCC
TTCTGGCGATCTGGGGGCGCGAGACAGGCTTCGGCGCGGCGAAGATGCCTTACGATGCCTTCGAGGTGCTGGGCACCAAG
GCGTTCATGTCGACGAAGCAGGATTTCTTCCGCACCGAGCTTTTGGCGGCGCTGGAAATCGTCCAACGCGGCCTCGCCCC
GGTCGGCGCTATGAAATCGTCCTGGGCCGGTGCCCTTGGCCAGCCGCAATTCATGCCGACCTCCTTCCTCAAGCATGCCG
TTGATTTCGACGGCGATGGCCGCGTCGACATCTGGAACTCGACGCCCGACGTACTCGCCTCGATCGCCAATTATCTCGTC
CACTATGGCTGGGTGAGGGGTCGCGGCTGGGGCTTCGAGGTGACCGTGCCCGAGAGCGTCTCCTGCTCGCTGGAAGGTCC
TGATCAGGGCAAGAAGATATCTGAGTGGGCTGGCATGGGCATCAAGCGCGTCGGTGGCAAGTCGTTTCCGGCGAGCGAGC
TGAAGGCGGAGGGTTTCCTGCTGATGCCGGCCGGCCGCAGCGGGCCGGCTTTCATCGCCACCCCCAATTTCTACGTGCTG
AAGGAATACAACACCAGCGACCTCTATGCGCTGTTCATCGGCCATGGCGCGGACCGCATTGCGCATGGCGACCAGAGTTT
TTCCGGCAGCTGGGGTCCGGTCGGCGATCTCCATCGCTCCGACATCGCCGCCCTGCAGCGGGCGCTGGAAGCCAAGGGCT
ATGATGTCGGCAGCGCCGACGGCCTGCCCGGTTTCAAGACCCGCCGTTCGATCGGCAAGTGGCAGACCAAGAATGGGCAG
GCGGCAACCTGCTTTCCCGATGCCGGCCTGGTCGCCTCGCTGAAATAA

Upstream 100 bases:

>100_bases
GCGTGCCGGTCGGCGGCGAACTCGACTATCTCGACGAGGGCACGCTCGCCGCCGCGCTACGCTCGCGAACGGCGTTTTGA
CGCAATTGGCGAGGGCCTAG

Downstream 100 bases:

>100_bases
CTCAAAAATCAGCAGATTTTTTTCGTTAGGCCGCCTCGATTGGCCGGCTTGTGCCTCGACGCGCCACCCGCCATTCTGCT
CCAATCGGATGTCGCACAAA

Product: transglycolase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTAFLRTIAFAFIAALLAVLLAAPASAAKIDDQFRAWLQNDLWPEAKTKGISKQTFDAAFDGIKPNLKLPDLVMPGEKPK
TPQKQHQAEFGSPGAYFAEKTVRAVTSGGRTREAANAKTLAAIEKRYGVPGEILLAIWGRETGFGAAKMPYDAFEVLGTK
AFMSTKQDFFRTELLAALEIVQRGLAPVGAMKSSWAGALGQPQFMPTSFLKHAVDFDGDGRVDIWNSTPDVLASIANYLV
HYGWVRGRGWGFEVTVPESVSCSLEGPDQGKKISEWAGMGIKRVGGKSFPASELKAEGFLLMPAGRSGPAFIATPNFYVL
KEYNTSDLYALFIGHGADRIAHGDQSFSGSWGPVGDLHRSDIAALQRALEAKGYDVGSADGLPGFKTRRSIGKWQTKNGQ
AATCFPDAGLVASLK

Sequences:

>Translated_415_residues
MTAFLRTIAFAFIAALLAVLLAAPASAAKIDDQFRAWLQNDLWPEAKTKGISKQTFDAAFDGIKPNLKLPDLVMPGEKPK
TPQKQHQAEFGSPGAYFAEKTVRAVTSGGRTREAANAKTLAAIEKRYGVPGEILLAIWGRETGFGAAKMPYDAFEVLGTK
AFMSTKQDFFRTELLAALEIVQRGLAPVGAMKSSWAGALGQPQFMPTSFLKHAVDFDGDGRVDIWNSTPDVLASIANYLV
HYGWVRGRGWGFEVTVPESVSCSLEGPDQGKKISEWAGMGIKRVGGKSFPASELKAEGFLLMPAGRSGPAFIATPNFYVL
KEYNTSDLYALFIGHGADRIAHGDQSFSGSWGPVGDLHRSDIAALQRALEAKGYDVGSADGLPGFKTRRSIGKWQTKNGQ
AATCFPDAGLVASLK
>Mature_414_residues
TAFLRTIAFAFIAALLAVLLAAPASAAKIDDQFRAWLQNDLWPEAKTKGISKQTFDAAFDGIKPNLKLPDLVMPGEKPKT
PQKQHQAEFGSPGAYFAEKTVRAVTSGGRTREAANAKTLAAIEKRYGVPGEILLAIWGRETGFGAAKMPYDAFEVLGTKA
FMSTKQDFFRTELLAALEIVQRGLAPVGAMKSSWAGALGQPQFMPTSFLKHAVDFDGDGRVDIWNSTPDVLASIANYLVH
YGWVRGRGWGFEVTVPESVSCSLEGPDQGKKISEWAGMGIKRVGGKSFPASELKAEGFLLMPAGRSGPAFIATPNFYVLK
EYNTSDLYALFIGHGADRIAHGDQSFSGSWGPVGDLHRSDIAALQRALEAKGYDVGSADGLPGFKTRRSIGKWQTKNGQA
ATCFPDAGLVASLK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=212, Percent_Identity=31.1320754716981, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44644; Mature: 44513

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFLRTIAFAFIAALLAVLLAAPASAAKIDDQFRAWLQNDLWPEAKTKGISKQTFDAAF
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
DGIKPNLKLPDLVMPGEKPKTPQKQHQAEFGSPGAYFAEKTVRAVTSGGRTREAANAKTL
CCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHCCHHHH
AAIEKRYGVPGEILLAIWGRETGFGAAKMPYDAFEVLGTKAFMSTKQDFFRTELLAALEI
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRGLAPVGAMKSSWAGALGQPQFMPTSFLKHAVDFDGDGRVDIWNSTPDVLASIANYLV
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH
HYGWVRGRGWGFEVTVPESVSCSLEGPDQGKKISEWAGMGIKRVGGKSFPASELKAEGFL
HHCCEECCCCEEEEECCCCCEEECCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHCCCCEE
LMPAGRSGPAFIATPNFYVLKEYNTSDLYALFIGHGADRIAHGDQSFSGSWGPVGDLHRS
EEECCCCCCEEEECCCEEEEEECCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCCCHHH
DIAALQRALEAKGYDVGSADGLPGFKTRRSIGKWQTKNGQAATCFPDAGLVASLK
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCEECCC
>Mature Secondary Structure 
TAFLRTIAFAFIAALLAVLLAAPASAAKIDDQFRAWLQNDLWPEAKTKGISKQTFDAAF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
DGIKPNLKLPDLVMPGEKPKTPQKQHQAEFGSPGAYFAEKTVRAVTSGGRTREAANAKTL
CCCCCCCCCCCEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHCCHHHH
AAIEKRYGVPGEILLAIWGRETGFGAAKMPYDAFEVLGTKAFMSTKQDFFRTELLAALEI
HHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQRGLAPVGAMKSSWAGALGQPQFMPTSFLKHAVDFDGDGRVDIWNSTPDVLASIANYLV
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHH
HYGWVRGRGWGFEVTVPESVSCSLEGPDQGKKISEWAGMGIKRVGGKSFPASELKAEGFL
HHCCEECCCCEEEEECCCCCEEECCCCCCCCHHHHHHCCCHHHCCCCCCCHHHHCCCCEE
LMPAGRSGPAFIATPNFYVLKEYNTSDLYALFIGHGADRIAHGDQSFSGSWGPVGDLHRS
EEECCCCCCEEEECCCEEEEEECCCCCEEEEEEECCHHHHCCCCCCCCCCCCCCCCCHHH
DIAALQRALEAKGYDVGSADGLPGFKTRRSIGKWQTKNGQAATCFPDAGLVASLK
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]