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The map label for this gene is guaD [H]

Identifier: 13474288

GI number: 13474288

Start: 4080313

End: 4081626

Strand: Direct

Name: guaD [H]

Synonym: mlr5142

Alternate gene names: 13474288

Gene position: 4080313-4081626 (Clockwise)

Preceding gene: 13474287

Following gene: 13474289

Centisome position: 57.99

GC content: 64.08

Gene sequence:

>1314_bases
ATGACCTCGACACTTCTGCGCGGCCGCACGCTGTCCTTCGTGCGCTGGCCCGAGACCATCGACGACCATTCGGCCTGGCG
TTACGAGGAGGATGGCGGCCTGCTGATAAGCGACGGTCGGATCATCGCCTCGGGCGCCTACCACGATGTCGAGAAGCAGG
CTGGTGAGGGGGCCAAAAAAATCGACCACCGGCCGCACCTCTTGCTGCCGGGCTTCATCGACACCCATGTGCATTTCCCG
CAGATGCAGGTCATCGCGTCCTATGGCGCCGAACTGCTCGACTGGTTGAACACCTACACTTTTCCGGAAGAAACGAAATT
CGCCAACGCGCAGCATGGCCGCCGCATCGCGCGACTCTTCCTCGACGAGATGGTGCGGCACGGCACCACGACGGTGGCTG
CCTACTGCTCGGTGCACAAGGCTTCCGCCGAAGCCTTTTTCGCCGAGTCGCACGACCGCAACATGCTCAACATCGCCGGC
AAGGTGATGATGGACCGCAATGCCCCGGACGGCGTGCTCGACACGCCGCAATCCGGCTACGACGACACCAAGGCGCTGAT
CAAGGAGTGGCACGGCAAGGGCCGCCAGCACTATGCCATCACCCCGCGCTTCGCCATCACCTCCTCGCCGGAGCAGATGG
AAATGGCCGGCGCGCTGTGCCGCGAGCATCCCGGCCTGCACATGCAGACGCATCTGTCGGAAAACCACGCCGAAATCGCC
TTCACGCAGCAGCTTTATCCCCGGTCGCGCGACTACACCGATGTCTACGAACATTACGGGTTGCTGGGCAAAAACAGCCT
GTTTGGCCACTGCATCCATCTGTCGGAACGTGAGGCGGATGCGCTGTCGCAGAGCGGCTCGGTGGCGGTGTTCTGCCCGA
CCTCGAACCTGTTCCTCGGCTCCGGCCTGTTCGACTACCAGCGCTTCCGGCGCAGGGACAAGCCGCTGCGTATCGCCGCC
GCCACCGATGTCGGCGGCGGCACCAACTATTCCATGCTGCGCACCATGGACGAGGGCTACAAGGTGATTGCGCTGAACGG
CGAGAAGCTCAACCCGTTCCAGTCCTTCTGGCAGATCACGCGCGGCAATGCCGAGGCGCTGTCGGTGGCCGACAAGATCG
GCACGCTGGATCAGGGTACCGACGCCGACATCGTCGTGCTCGACGCCAGGGCGACGCCGGCGATGCGGCTCAGGATGGAA
ACGGCACAGACGCTGGCGCAGGAATTGTTCCTGCTGCAGACGCTGGGCGACGACCGCGCCGTGCGCGAGGTCTATGTCGC
GGGGCGGGCCGCCAAGAGCGACATGGCGAATTAG

Upstream 100 bases:

>100_bases
GCCGGCCGCAGCCACGCCATGCCGCCCGCCAATGTCAGCCAGATCACCGACAAGGAACGGGCATTGCTGGTGGCCTGGTT
CGAAGGCGCGGGGAAATAGC

Downstream 100 bases:

>100_bases
AGCGTCGGGTGCGTTCATTGCTTGACCCGGCGGCAGCCATCGGTCAAAGCGTGCGGCGAAGTTGACAGGGGAACGACATG
GCCGTTGCTGACGACATCGC

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTSTLLRGRTLSFVRWPETIDDHSAWRYEEDGGLLISDGRIIASGAYHDVEKQAGEGAKKIDHRPHLLLPGFIDTHVHFP
QMQVIASYGAELLDWLNTYTFPEETKFANAQHGRRIARLFLDEMVRHGTTTVAAYCSVHKASAEAFFAESHDRNMLNIAG
KVMMDRNAPDGVLDTPQSGYDDTKALIKEWHGKGRQHYAITPRFAITSSPEQMEMAGALCREHPGLHMQTHLSENHAEIA
FTQQLYPRSRDYTDVYEHYGLLGKNSLFGHCIHLSEREADALSQSGSVAVFCPTSNLFLGSGLFDYQRFRRRDKPLRIAA
ATDVGGGTNYSMLRTMDEGYKVIALNGEKLNPFQSFWQITRGNAEALSVADKIGTLDQGTDADIVVLDARATPAMRLRME
TAQTLAQELFLLQTLGDDRAVREVYVAGRAAKSDMAN

Sequences:

>Translated_437_residues
MTSTLLRGRTLSFVRWPETIDDHSAWRYEEDGGLLISDGRIIASGAYHDVEKQAGEGAKKIDHRPHLLLPGFIDTHVHFP
QMQVIASYGAELLDWLNTYTFPEETKFANAQHGRRIARLFLDEMVRHGTTTVAAYCSVHKASAEAFFAESHDRNMLNIAG
KVMMDRNAPDGVLDTPQSGYDDTKALIKEWHGKGRQHYAITPRFAITSSPEQMEMAGALCREHPGLHMQTHLSENHAEIA
FTQQLYPRSRDYTDVYEHYGLLGKNSLFGHCIHLSEREADALSQSGSVAVFCPTSNLFLGSGLFDYQRFRRRDKPLRIAA
ATDVGGGTNYSMLRTMDEGYKVIALNGEKLNPFQSFWQITRGNAEALSVADKIGTLDQGTDADIVVLDARATPAMRLRME
TAQTLAQELFLLQTLGDDRAVREVYVAGRAAKSDMAN
>Mature_436_residues
TSTLLRGRTLSFVRWPETIDDHSAWRYEEDGGLLISDGRIIASGAYHDVEKQAGEGAKKIDHRPHLLLPGFIDTHVHFPQ
MQVIASYGAELLDWLNTYTFPEETKFANAQHGRRIARLFLDEMVRHGTTTVAAYCSVHKASAEAFFAESHDRNMLNIAGK
VMMDRNAPDGVLDTPQSGYDDTKALIKEWHGKGRQHYAITPRFAITSSPEQMEMAGALCREHPGLHMQTHLSENHAEIAF
TQQLYPRSRDYTDVYEHYGLLGKNSLFGHCIHLSEREADALSQSGSVAVFCPTSNLFLGSGLFDYQRFRRRDKPLRIAAA
TDVGGGTNYSMLRTMDEGYKVIALNGEKLNPFQSFWQITRGNAEALSVADKIGTLDQGTDADIVVLDARATPAMRLRMET
AQTLAQELFLLQTLGDDRAVREVYVAGRAAKSDMAN

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=435, Percent_Identity=28.9655172413793, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1789249, Length=422, Percent_Identity=45.260663507109, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6319963, Length=336, Percent_Identity=33.6309523809524, Blast_Score=184, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24643849, Length=433, Percent_Identity=30.4849884526559, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 48813; Mature: 48681

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTLLRGRTLSFVRWPETIDDHSAWRYEEDGGLLISDGRIIASGAYHDVEKQAGEGAKK
CCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCEEEECCCHHHHHHHHCCCHHH
IDHRPHLLLPGFIDTHVHFPQMQVIASYGAELLDWLNTYTFPEETKFANAQHGRRIARLF
HCCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LDEMVRHGTTTVAAYCSVHKASAEAFFAESHDRNMLNIAGKVMMDRNAPDGVLDTPQSGY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCCCCCCCCCCCCC
DDTKALIKEWHGKGRQHYAITPRFAITSSPEQMEMAGALCREHPGLHMQTHLSENHAEIA
CHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEE
FTQQLYPRSRDYTDVYEHYGLLGKNSLFGHCIHLSEREADALSQSGSVAVFCPTSNLFLG
EEHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEEECCCCCEEC
SGLFDYQRFRRRDKPLRIAAATDVGGGTNYSMLRTMDEGYKVIALNGEKLNPFQSFWQIT
CCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
RGNAEALSVADKIGTLDQGTDADIVVLDARATPAMRLRMETAQTLAQELFLLQTLGDDRA
CCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
VREVYVAGRAAKSDMAN
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TSTLLRGRTLSFVRWPETIDDHSAWRYEEDGGLLISDGRIIASGAYHDVEKQAGEGAKK
CCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCEEEECCCHHHHHHHHCCCHHH
IDHRPHLLLPGFIDTHVHFPQMQVIASYGAELLDWLNTYTFPEETKFANAQHGRRIARLF
HCCCCCEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LDEMVRHGTTTVAAYCSVHKASAEAFFAESHDRNMLNIAGKVMMDRNAPDGVLDTPQSGY
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEECCCCCCCCCCCCCCCC
DDTKALIKEWHGKGRQHYAITPRFAITSSPEQMEMAGALCREHPGLHMQTHLSENHAEIA
CHHHHHHHHHHCCCCEEEEECCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEE
FTQQLYPRSRDYTDVYEHYGLLGKNSLFGHCIHLSEREADALSQSGSVAVFCPTSNLFLG
EEHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCEEEEECCCCCEEC
SGLFDYQRFRRRDKPLRIAAATDVGGGTNYSMLRTMDEGYKVIALNGEKLNPFQSFWQIT
CCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
RGNAEALSVADKIGTLDQGTDADIVVLDARATPAMRLRMETAQTLAQELFLLQTLGDDRA
CCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
VREVYVAGRAAKSDMAN
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]