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The map label for this gene is hisH

Identifier: 13474182

GI number: 13474182

Start: 3971641

End: 3972291

Strand: Direct

Name: hisH

Synonym: mlr5011

Alternate gene names: 13474182

Gene position: 3971641-3972291 (Clockwise)

Preceding gene: 13474181

Following gene: 13474183

Centisome position: 56.45

GC content: 66.36

Gene sequence:

>651_bases
ATGCGGGTAGCAATCATCGACTACGGCTCGGGCAATCTGCGCTCGGCCACCAAGGCCTTCGAGCGCGCCGCGCGCGAAGG
AGGCATATCGGCCGAGATCGACCTGACGGCCGATGCCGACCGGGTGCGCACGGCCGACCGCATCGTCCTGCCTGGCGTCG
GCGCCTATGCCGACTGCGCGGCCGGCCTGCGTGCCGTCGACGGCATGTGGGAAGCGGTCGAGGACGTCGCCATCGCTCAG
AGCCGGCCCTTCCTCGGCATCTGCGTCGGTATGCAGCTGATGTCTGAGCGCGGCCTGGAAAAGACCGTCACCCATGGCTT
CGGCTGGATCTCGGGCGACGTCAAGGAGATCACGCCCACCGATCCGGCGCTGAAAATCCCGCAGATCGGCTGGAACACGA
TCGAGCTCAGGCGCCAACACCCGCTGTTTGCCGGCATCCCGACCGGACCAGAGGGGCTGCACGCCTATTTCGTGCATTCC
TACCATCTCGACGCGAAAAAGCCGGACGAGATTCTGGCCGTCGCCGACTATGGCGGTCCGGTGACGGCGGCCGTCGCCCG
CGACAATCTCGTCGGCACGCAGTTCCATCCCGAAAAGAGCCAGGCGCTGGGTCTGGCGCTGATCACCAATTTCCTGCGGT
GGAGGCCCTGA

Upstream 100 bases:

>100_bases
ACCACACCCGCGCCGCGCATCGTTCCGGATGCCGCCCTGGCGCGCCGGGCGCAGCCGGGCATAGCGCTGGGCCTGACCCA
CATACCGGGACGGCCCTGAC

Downstream 100 bases:

>100_bases
TGGTGAGCAAGAAAGGGTATTGGATGGTGATGCTCACGGTGACCGATGCCGAGGGTTACCAGCGCTATCGTGAAGCCATT
GGCGATGCCATTTCGCACTT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MRVAIIDYGSGNLRSATKAFERAAREGGISAEIDLTADADRVRTADRIVLPGVGAYADCAAGLRAVDGMWEAVEDVAIAQ
SRPFLGICVGMQLMSERGLEKTVTHGFGWISGDVKEITPTDPALKIPQIGWNTIELRRQHPLFAGIPTGPEGLHAYFVHS
YHLDAKKPDEILAVADYGGPVTAAVARDNLVGTQFHPEKSQALGLALITNFLRWRP

Sequences:

>Translated_216_residues
MRVAIIDYGSGNLRSATKAFERAAREGGISAEIDLTADADRVRTADRIVLPGVGAYADCAAGLRAVDGMWEAVEDVAIAQ
SRPFLGICVGMQLMSERGLEKTVTHGFGWISGDVKEITPTDPALKIPQIGWNTIELRRQHPLFAGIPTGPEGLHAYFVHS
YHLDAKKPDEILAVADYGGPVTAAVARDNLVGTQFHPEKSQALGLALITNFLRWRP
>Mature_216_residues
MRVAIIDYGSGNLRSATKAFERAAREGGISAEIDLTADADRVRTADRIVLPGVGAYADCAAGLRAVDGMWEAVEDVAIAQ
SRPFLGICVGMQLMSERGLEKTVTHGFGWISGDVKEITPTDPALKIPQIGWNTIELRRQHPLFAGIPTGPEGLHAYFVHS
YHLDAKKPDEILAVADYGGPVTAAVARDNLVGTQFHPEKSQALGLALITNFLRWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=214, Percent_Identity=38.785046728972, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=222, Percent_Identity=31.981981981982, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_RHILO (Q98CT5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105750.1
- ProteinModelPortal:   Q98CT5
- SMR:   Q98CT5
- GeneID:   1228411
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr5011
- NMPDR:   fig|266835.1.peg.3854
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13146
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23331; Mature: 23331

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 196-196 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREGGISAEIDLTADADRVRTADRIVLPGVGAYADCA
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCEEEECCCCCHHHHH
AGLRAVDGMWEAVEDVAIAQSRPFLGICVGMQLMSERGLEKTVTHGFGWISGDVKEITPT
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHCCCEECCCCEECCCC
DPALKIPQIGWNTIELRRQHPLFAGIPTGPEGLHAYFVHSYHLDAKKPDEILAVADYGGP
CCCEECCCCCCCEEEEECCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCC
VTAAVARDNLVGTQFHPEKSQALGLALITNFLRWRP
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVAIIDYGSGNLRSATKAFERAAREGGISAEIDLTADADRVRTADRIVLPGVGAYADCA
CEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCEEEECCCCCHHHHH
AGLRAVDGMWEAVEDVAIAQSRPFLGICVGMQLMSERGLEKTVTHGFGWISGDVKEITPT
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCHHHHHHCCCEECCCCEECCCC
DPALKIPQIGWNTIELRRQHPLFAGIPTGPEGLHAYFVHSYHLDAKKPDEILAVADYGGP
CCCEECCCCCCCEEEEECCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCEEEEECCCCC
VTAAVARDNLVGTQFHPEKSQALGLALITNFLRWRP
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968