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The map label for this gene is dapE

Identifier: 13474060

GI number: 13474060

Start: 3854474

End: 3855667

Strand: Direct

Name: dapE

Synonym: mlr4849

Alternate gene names: 13474060

Gene position: 3854474-3855667 (Clockwise)

Preceding gene: 13474058

Following gene: 13474061

Centisome position: 54.78

GC content: 64.91

Gene sequence:

>1194_bases
ATGACGCTGCCGACCGACCCCGCCGCAAACCTCGCCGCCCTTATCCGTTGCGCCTCGGTGACTCCAGCCGAAGGCGGCGC
GCTGGGCGCGCTGGAAACGATGCTGAAGCCGCTCGGCTTCCTGGTCGACCGGCCCGTCTTTTCGGAAGACGGCACGCCGG
ACATCGAGAACCTCTATGCGAGGCGGTCCGGCAACGGCCCGCATCTGATGTTCGCCGGCCATACCGATGTCGTGCCGGTC
GGCGACGAAGCCGCCTGGACGCATCCGCCTTTCGCCGCCGAGATCGCCAATGGCGAGATGTATGGCCGTGGCGCCGTCGA
CATGAAGGGCGGCATTGCCTGCTTCGTCGCGGCGATAGCGCGCCATGTCGAGAACAATGGCGGCCCCAAGGGCTCGGTCT
CGCTGCTGATCACCGGTGACGAGGAAGGCCCGGCCATCAACGGCACGGTCAAGCTGCTCGAATGGGCCGCTTCCAGGGGC
GAGAAATGGGATGCCTCGATCGTTGGCGAGCCGACCAACCCGGACACGCTTGGCGACATGATCAAGATCGGCCGCCGCGG
CTCGCTGTCCGGTTCGATCACGGTCAATGGCCGTCAGGGCCATGCCGCCTATCCGCAGCTTGCCGACAATCCGGTGCGCG
GCTTGATGGGCCTGGTCGATGCCCTGCTGCATCCCGTCTTCGACAAGGGAACCAAGGATTTCCAACCGACCAATCTGGAG
GTCACCTCCATCGATGTCGGCAACCCGGCCACCAATGTCATCCCCGCGAAGGCGACCGCGACCTTCAACATCCGCTTCAA
CGACACCTGGACGGCCGAGACCGTCCAGGCCGAAATCCACAACCGGCTCGACCAGGCGGCGAAGCGCAAGAAGTATCGGC
CGGGCAAGAAGACACCGGTCGACTATGACCTCGTCTGGCGCGACCGGCCGAGCCATGTCTTCCTGACCCGCGACGACAAG
TTGGTCGACACGCTTGCCGGCTCGATCAAGGCGGCGGTCGGCAAGGAGCCGACATTGTCCACTTCGGGCGGCACGTCGGA
TGCGCGCTTCATCAAGGATTACTGCCCGGTGGTCGAGTTCGGCCTGGTCGGCAAGACCATGCATATGGTCGACGAGCGCG
TCGCCCTTGCCGATCTCGAAACGCTGACGCGGATCTACCAGCGCTTCATCGAAGACTGGTTCGGGCAGGGCTGA

Upstream 100 bases:

>100_bases
TACGACATGAAAACCATAATGTCGGCGGCATCGTCTGCCATTGGGCTGGCCCCCATGTCTGCCCGTGACAGGCCCTCAGC
TTTCGACTATCGCTTTTGCC

Downstream 100 bases:

>100_bases
GCAGCATGCTTTCGGCGGATGAAACCCAAGCTTCGCTCACTGGTGCCTGGCGGCTGATGCTCGGCAAGGTCGACGGCCTG
CGCCTGCTCGACCTGTCGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MTLPTDPAANLAALIRCASVTPAEGGALGALETMLKPLGFLVDRPVFSEDGTPDIENLYARRSGNGPHLMFAGHTDVVPV
GDEAAWTHPPFAAEIANGEMYGRGAVDMKGGIACFVAAIARHVENNGGPKGSVSLLITGDEEGPAINGTVKLLEWAASRG
EKWDASIVGEPTNPDTLGDMIKIGRRGSLSGSITVNGRQGHAAYPQLADNPVRGLMGLVDALLHPVFDKGTKDFQPTNLE
VTSIDVGNPATNVIPAKATATFNIRFNDTWTAETVQAEIHNRLDQAAKRKKYRPGKKTPVDYDLVWRDRPSHVFLTRDDK
LVDTLAGSIKAAVGKEPTLSTSGGTSDARFIKDYCPVVEFGLVGKTMHMVDERVALADLETLTRIYQRFIEDWFGQG

Sequences:

>Translated_397_residues
MTLPTDPAANLAALIRCASVTPAEGGALGALETMLKPLGFLVDRPVFSEDGTPDIENLYARRSGNGPHLMFAGHTDVVPV
GDEAAWTHPPFAAEIANGEMYGRGAVDMKGGIACFVAAIARHVENNGGPKGSVSLLITGDEEGPAINGTVKLLEWAASRG
EKWDASIVGEPTNPDTLGDMIKIGRRGSLSGSITVNGRQGHAAYPQLADNPVRGLMGLVDALLHPVFDKGTKDFQPTNLE
VTSIDVGNPATNVIPAKATATFNIRFNDTWTAETVQAEIHNRLDQAAKRKKYRPGKKTPVDYDLVWRDRPSHVFLTRDDK
LVDTLAGSIKAAVGKEPTLSTSGGTSDARFIKDYCPVVEFGLVGKTMHMVDERVALADLETLTRIYQRFIEDWFGQG
>Mature_396_residues
TLPTDPAANLAALIRCASVTPAEGGALGALETMLKPLGFLVDRPVFSEDGTPDIENLYARRSGNGPHLMFAGHTDVVPVG
DEAAWTHPPFAAEIANGEMYGRGAVDMKGGIACFVAAIARHVENNGGPKGSVSLLITGDEEGPAINGTVKLLEWAASRGE
KWDASIVGEPTNPDTLGDMIKIGRRGSLSGSITVNGRQGHAAYPQLADNPVRGLMGLVDALLHPVFDKGTKDFQPTNLEV
TSIDVGNPATNVIPAKATATFNIRFNDTWTAETVQAEIHNRLDQAAKRKKYRPGKKTPVDYDLVWRDRPSHVFLTRDDKL
VDTLAGSIKAAVGKEPTLSTSGGTSDARFIKDYCPVVEFGLVGKTMHMVDERVALADLETLTRIYQRFIEDWFGQG

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=380, Percent_Identity=40.2631578947368, Blast_Score=264, Evalue=6e-72,
Organism=Escherichia coli, GI1790395, Length=349, Percent_Identity=25.7879656160458, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_RHILO (Q98D57)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105628.1
- ProteinModelPortal:   Q98D57
- SMR:   Q98D57
- MEROPS:   M20.010
- GeneID:   1228289
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4849
- NMPDR:   fig|266835.1.peg.3732
- HOGENOM:   HBG728841
- OMA:   INNICAE
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42630; Mature: 42498

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 76-76 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPTDPAANLAALIRCASVTPAEGGALGALETMLKPLGFLVDRPVFSEDGTPDIENLYA
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RRSGNGPHLMFAGHTDVVPVGDEAAWTHPPFAAEIANGEMYGRGAVDMKGGIACFVAAIA
HCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEECCCCEECCCCCCCCCHHHHHHHHHH
RHVENNGGPKGSVSLLITGDEEGPAINGTVKLLEWAASRGEKWDASIVGEPTNPDTLGDM
HHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
IKIGRRGSLSGSITVNGRQGHAAYPQLADNPVRGLMGLVDALLHPVFDKGTKDFQPTNLE
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VTSIDVGNPATNVIPAKATATFNIRFNDTWTAETVQAEIHNRLDQAAKRKKYRPGKKTPV
EEEEECCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DYDLVWRDRPSHVFLTRDDKLVDTLAGSIKAAVGKEPTLSTSGGTSDARFIKDYCPVVEF
CEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHH
GLVGKTMHMVDERVALADLETLTRIYQRFIEDWFGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLPTDPAANLAALIRCASVTPAEGGALGALETMLKPLGFLVDRPVFSEDGTPDIENLYA
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RRSGNGPHLMFAGHTDVVPVGDEAAWTHPPFAAEIANGEMYGRGAVDMKGGIACFVAAIA
HCCCCCCEEEEECCCCEEECCCCCCCCCCCCCEEECCCCEECCCCCCCCCHHHHHHHHHH
RHVENNGGPKGSVSLLITGDEEGPAINGTVKLLEWAASRGEKWDASIVGEPTNPDTLGDM
HHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHH
IKIGRRGSLSGSITVNGRQGHAAYPQLADNPVRGLMGLVDALLHPVFDKGTKDFQPTNLE
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VTSIDVGNPATNVIPAKATATFNIRFNDTWTAETVQAEIHNRLDQAAKRKKYRPGKKTPV
EEEEECCCCCCCEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DYDLVWRDRPSHVFLTRDDKLVDTLAGSIKAAVGKEPTLSTSGGTSDARFIKDYCPVVEF
CEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCHHHH
GLVGKTMHMVDERVALADLETLTRIYQRFIEDWFGQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968