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The map label for this gene is vatD [H]

Identifier: 13474031

GI number: 13474031

Start: 3828747

End: 3829385

Strand: Direct

Name: vatD [H]

Synonym: mlr4814

Alternate gene names: 13474031

Gene position: 3828747-3829385 (Clockwise)

Preceding gene: 13474030

Following gene: 13474032

Centisome position: 54.42

GC content: 61.35

Gene sequence:

>639_bases
ATGGACGGACCCAACCCCAACATCAAGCACCCGATCCCGATGCACACCCGCGTCGGTTTCCTGAAGCCGCTGGTGACGGA
GCCGAACATCGAGATCGGCGACTTCACCTATTACGACGACCCGGACGGACCAGACAAATTCGCGCAGAAATGCGTGCTGC
ACCACTACCCCTTCATCGGCGACAAGCTCATCATCGGCAAGTTCTGCGCCATCGCCGAAGGCGCGCGCTTCATCATGAAC
GGCGCCAACCACGCAATGTCCGGCTTTTCGACCTATCCGTTCAACATTTTCGGCCATGGCTGGGAAAAGGGCTTCGACCC
GGCGACATGGTCGAAGGAGGTGCGCGGCGACACGGTGGTCGGCAGCGACGTCTGGATCGGCATGGAGGCGGTGATCCTGC
CCGGCGTGGAAATCGGCCATGGCGCCATCATCGCCGCCAAATCGGTTGTGACGCACGATGTGCCCCCCTATGCCATAGTC
GCCGGCAACGCGGCCAAGGTGGTGAAGATGCGTTTCGACGACAGGACGATCAGGCGGTTGCTGGCATTGGCCTGGTGGCA
TTGGCCCGTGGACAAGATCGGCCGCAACCTCGATGCCATCAGGGGCGCCAATATCTCTCTTCTGGAGGCAGCAGCTTGA

Upstream 100 bases:

>100_bases
CGCTCCCCGGCCGATTGGTGCTATAGGGCGGATCACGTTATTTTGACCAGGCGCCTCCGGTAGCGGATGCGCGGGTCTGC
CAATTCCCGGACGAACCGAC

Downstream 100 bases:

>100_bases
ACGCTCTGAACAGCACTGTGATCACCACCGACCTGTTCACGCGCGGGTGGATCTTCATCCGCGGCGTGCCGGCCATGAAG
TTCCTGCCGCCGGAAGGGCC

Product: streptogramin A acetyl transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: Virginiamycin acetyltransferase D; Vat(D) [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MDGPNPNIKHPIPMHTRVGFLKPLVTEPNIEIGDFTYYDDPDGPDKFAQKCVLHHYPFIGDKLIIGKFCAIAEGARFIMN
GANHAMSGFSTYPFNIFGHGWEKGFDPATWSKEVRGDTVVGSDVWIGMEAVILPGVEIGHGAIIAAKSVVTHDVPPYAIV
AGNAAKVVKMRFDDRTIRRLLALAWWHWPVDKIGRNLDAIRGANISLLEAAA

Sequences:

>Translated_212_residues
MDGPNPNIKHPIPMHTRVGFLKPLVTEPNIEIGDFTYYDDPDGPDKFAQKCVLHHYPFIGDKLIIGKFCAIAEGARFIMN
GANHAMSGFSTYPFNIFGHGWEKGFDPATWSKEVRGDTVVGSDVWIGMEAVILPGVEIGHGAIIAAKSVVTHDVPPYAIV
AGNAAKVVKMRFDDRTIRRLLALAWWHWPVDKIGRNLDAIRGANISLLEAAA
>Mature_212_residues
MDGPNPNIKHPIPMHTRVGFLKPLVTEPNIEIGDFTYYDDPDGPDKFAQKCVLHHYPFIGDKLIIGKFCAIAEGARFIMN
GANHAMSGFSTYPFNIFGHGWEKGFDPATWSKEVRGDTVVGSDVWIGMEAVILPGVEIGHGAIIAAKSVVTHDVPPYAIV
AGNAAKVVKMRFDDRTIRRLLALAWWHWPVDKIGRNLDAIRGANISLLEAAA

Specific function: Inactivates the A compounds of streptogramin antibiotics by acetylation, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23300; Mature: 23300

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGPNPNIKHPIPMHTRVGFLKPLVTEPNIEIGDFTYYDDPDGPDKFAQKCVLHHYPFIG
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHCCCCCC
DKLIIGKFCAIAEGARFIMNGANHAMSGFSTYPFNIFGHGWEKGFDPATWSKEVRGDTVV
CCHHHHHHHHHHHCCCEEECCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCEEE
GSDVWIGMEAVILPGVEIGHGAIIAAKSVVTHDVPPYAIVAGNAAKVVKMRFDDRTIRRL
CCCEEECEEEEEECCCEECCCEEEEEHHHHHCCCCCEEEEECCCEEEEEEECCHHHHHHH
LALAWWHWPVDKIGRNLDAIRGANISLLEAAA
HHHHHHCCCHHHHCCCHHHHCCCCEEEEECCC
>Mature Secondary Structure
MDGPNPNIKHPIPMHTRVGFLKPLVTEPNIEIGDFTYYDDPDGPDKFAQKCVLHHYPFIG
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCEEECCCCCCHHHHHHHHHHHCCCCCC
DKLIIGKFCAIAEGARFIMNGANHAMSGFSTYPFNIFGHGWEKGFDPATWSKEVRGDTVV
CCHHHHHHHHHHHCCCEEECCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHCCCEEE
GSDVWIGMEAVILPGVEIGHGAIIAAKSVVTHDVPPYAIVAGNAAKVVKMRFDDRTIRRL
CCCEEECEEEEEECCCEECCCEEEEEHHHHHCCCCCEEEEECCCEEEEEEECCHHHHHHH
LALAWWHWPVDKIGRNLDAIRGANISLLEAAA
HHHHHHCCCHHHHCCCHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8257133 [H]