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The map label for this gene is hyuA [H]

Identifier: 13474010

GI number: 13474010

Start: 3805669

End: 3807753

Strand: Direct

Name: hyuA [H]

Synonym: mlr4786

Alternate gene names: 13474010

Gene position: 3805669-3807753 (Clockwise)

Preceding gene: 13474004

Following gene: 13474011

Centisome position: 54.09

GC content: 65.95

Gene sequence:

>2085_bases
ATGAAAGAGAATTTTTCGGTGCAGCCGCCGGATTCCCTGGGCAGCGTCGCAGCCGGCATCGATGTCGGCGGGACCTTCAC
CGACCTGCTTCTGATCGACGGCAGAGACGGCGGCAAGGTGCATATCGCCAAGACCCCGACGACGGTCGATAACCAGGCCT
TCGGTGTGGTTTCGGCACTTGGTGCCACCGGATTTGCCATCGACGGCATCGACCTCATCGTGCATGGCACGACCACTACC
ACCAATGCCGTGCTGGAGCGCCGGCTGGCCAGGACCGGCATGATCACCACGCGCGGCTTTCGCGACGTGATCGAGCTTGG
CCGGCGCACGCGGCCGCAACCTTATGGCATGACCGGTACTTTCGTTCCCGTCATCCCGCGCAATCTCAGGCTCGAAGTGT
CGGAGCGCGTCGAGGCTTCCGGTGCGGTGCGCACGCCGCTCGACGAGGCCGAAATGCGCGAGGCGGTGACGGCGCTGATC
GCCGCCGGCTGCGAATCCCTCGTCATCCATTTCCTGCATTCCTACGCCAACCCGGCGCATGAGCGGCGCGCCGCCGAGAT
TGCCGCCGAGCTCTGGCCGAACGGCTACATCACCACGGGCCATGCGCTGCTGTCGGAAGCGCGCGAATTCGAGCGTGGCG
TCACAGCGTCGGTCAACGCCTCCGTGCAGCCGATTCTCGAACGCTATGTCGAGCGGCTGCGCAAGGAATTGGCCGCCAAA
GGCTATGCCCGGGACTTCCTGATCATGAACGGCAATGGCGGCATGATCTCGGCCCGCTTCGTCACACGTGAATCGGCCAA
GACGGTGATGTCCGGCCCGGCCTCCGGCGTCATCGCCGCCGCCTATACGGGCAAACGCGCCGGCTTCGAAAACCTCGTCA
CCTACGACATGGGCGGCACCTCGACCGATGTGGCGCTGATCCGCAACGCCGAGCCGGCGGTCTCCAACGAGATCGAGATC
GAATATGCCATGCCCATCCATGTGCCGATGGTGGCGGTGCACACGGTCGGCGCCGGCGGCGGCTCGATCGCCCGTGTCGA
CGCGGCCGGCCTGATCCAGATTGGTCCGGAAAGCGCCGGCGCCAATCCCGGCCCGATCTGCTATGGCCGCGGCGGCCTGG
AGCCGACCATCACCGACGCCAATCTGGTGCTCGGCCGGCTGGCGCCGAAGAAGCTGCTGGCCGTCGACAACCCGGTCACT
GTGGAGCGCGTCACCGGCATTTTCGCGGACAAGATCGGCAAGGCGACTGGACTGTCCGGCGTCGAGGCAGCAGGTGCTGT
GCTGAGGCTCGGCAACATGAAGATGGCCGGCGCCATCCGCATGGTCTCGGTGTCGCGAGGCCATGACCCGCGCGATTTCG
CGCTGTTTGCTTTCGGCGGCGCCGGGCCGCTGCATGCGACCGCCCTGGCGCGCGAACTCGGCCTGCCCCGTGTGCTGGTG
CCGGCGCGTCCAGGCATCACCAATGCGCTCGGCTGCGTCGTCGCCGATCTGCGTCATGACTTCGTCAACACCATCAACCA
GCCGGTTGCCGTGCTCGACGAAACCCAGCTGCACCATGTATTGGAACGGCACCGAAACGAAGGCGAGCAGCTGATCGGCA
AGGAAGCGGTGAAGCCCGAGACGATCCGCGTCACCCACTCCGCCGACATGCAGTTTGTCGGCCAGACCCACATCATCAAC
GTGCCGCTGCCGTCCTCGACCGTGACCCGGCAGGAGTTGCAGGCGCTGTTCGAAAAGGCCTATTTCGCCCGCTTCAAGGT
CGAGCTGCCGGAAATCCGCGCCAACCTGGTCAACCTCAACACCTCGGTCACCGGCGTGCGCCCGGCGATCGACCTGTCGC
GCCTGATCGACCCGGCCGGTCGCGCGAAAACGCTGGACGAGGCGCGGCGCGAGATCCGGCCGGTCTGGTATGCGGGCCAG
TGGCACGACACGCCGGTATATGCACGCGAAAAATTGCCGCTCGATGCCGTCATCGAAGGCCCGGCGATCCTCGAACAGAT
GGACGCCACCACCGTGCTCGAACCCGGCGACCGCGCCCGGTCGGATGCCGACGGCAACATCATCATCGACATTGGCGAGG
CCTGA

Upstream 100 bases:

>100_bases
AATTTATTAGTATACAAACGATATTTGCGTCAAGATACCGCCGCGAAACACCTTTCCAAACTGTGCCATATCCGTAGTCC
CAGCATTTCGGGTTTCGACA

Downstream 100 bases:

>100_bases
TATGCCTGACATTGATATGGCAAAGCTCGACGCCATCACGCTTTCGGTCCTCCAGGCGGCCTTGCAGCAGGTCTGCGATG
AGATGGACCTGACCTTCTCC

Product: N-methylhydantoinase A (mikimopine-lactam hydrogenase)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 694; Mature: 694

Protein sequence:

>694_residues
MKENFSVQPPDSLGSVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSALGATGFAIDGIDLIVHGTTTT
TNAVLERRLARTGMITTRGFRDVIELGRRTRPQPYGMTGTFVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALI
AAGCESLVIHFLHSYANPAHERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELAAK
GYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMGGTSTDVALIRNAEPAVSNEIEI
EYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPESAGANPGPICYGRGGLEPTITDANLVLGRLAPKKLLAVDNPVT
VERVTGIFADKIGKATGLSGVEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGGAGPLHATALARELGLPRVLV
PARPGITNALGCVVADLRHDFVNTINQPVAVLDETQLHHVLERHRNEGEQLIGKEAVKPETIRVTHSADMQFVGQTHIIN
VPLPSSTVTRQELQALFEKAYFARFKVELPEIRANLVNLNTSVTGVRPAIDLSRLIDPAGRAKTLDEARREIRPVWYAGQ
WHDTPVYAREKLPLDAVIEGPAILEQMDATTVLEPGDRARSDADGNIIIDIGEA

Sequences:

>Translated_694_residues
MKENFSVQPPDSLGSVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSALGATGFAIDGIDLIVHGTTTT
TNAVLERRLARTGMITTRGFRDVIELGRRTRPQPYGMTGTFVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALI
AAGCESLVIHFLHSYANPAHERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELAAK
GYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMGGTSTDVALIRNAEPAVSNEIEI
EYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPESAGANPGPICYGRGGLEPTITDANLVLGRLAPKKLLAVDNPVT
VERVTGIFADKIGKATGLSGVEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGGAGPLHATALARELGLPRVLV
PARPGITNALGCVVADLRHDFVNTINQPVAVLDETQLHHVLERHRNEGEQLIGKEAVKPETIRVTHSADMQFVGQTHIIN
VPLPSSTVTRQELQALFEKAYFARFKVELPEIRANLVNLNTSVTGVRPAIDLSRLIDPAGRAKTLDEARREIRPVWYAGQ
WHDTPVYAREKLPLDAVIEGPAILEQMDATTVLEPGDRARSDADGNIIIDIGEA
>Mature_694_residues
MKENFSVQPPDSLGSVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSALGATGFAIDGIDLIVHGTTTT
TNAVLERRLARTGMITTRGFRDVIELGRRTRPQPYGMTGTFVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALI
AAGCESLVIHFLHSYANPAHERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELAAK
GYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMGGTSTDVALIRNAEPAVSNEIEI
EYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPESAGANPGPICYGRGGLEPTITDANLVLGRLAPKKLLAVDNPVT
VERVTGIFADKIGKATGLSGVEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGGAGPLHATALARELGLPRVLV
PARPGITNALGCVVADLRHDFVNTINQPVAVLDETQLHHVLERHRNEGEQLIGKEAVKPETIRVTHSADMQFVGQTHIIN
VPLPSSTVTRQELQALFEKAYFARFKVELPEIRANLVNLNTSVTGVRPAIDLSRLIDPAGRAKTLDEARREIRPVWYAGQ
WHDTPVYAREKLPLDAVIEGPAILEQMDATTVLEPGDRARSDADGNIIIDIGEA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=664, Percent_Identity=29.066265060241, Blast_Score=224, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI133901900, Length=727, Percent_Identity=25.171939477304, Blast_Score=179, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322634, Length=663, Percent_Identity=28.3559577677225, Blast_Score=210, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550492, Length=745, Percent_Identity=27.6510067114094, Blast_Score=227, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 74105; Mature: 74105

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENFSVQPPDSLGSVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSAL
CCCCCCCCCCHHHCCHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHH
GATGFAIDGIDLIVHGTTTTTNAVLERRLARTGMITTRGFRDVIELGRRTRPQPYGMTGT
CCCCEEECCEEEEEECCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCCC
FVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALIAAGCESLVIHFLHSYANPAH
CCEECCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
ERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELAAK
HHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
GYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMGGT
CCCCEEEEECCCCCEEEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCC
STDVALIRNAEPAVSNEIEIEYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPESAG
CCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEECCCCEEEECCCCCC
ANPGPICYGRGGLEPTITDANLVLGRLAPKKLLAVDNPVTVERVTGIFADKIGKATGLSG
CCCCCEEECCCCCCCCCCCHHHHHHHCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCC
VEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGGAGPLHATALARELGLPRVLV
HHHHHHHHEECCEEECCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
PARPGITNALGCVVADLRHDFVNTINQPVAVLDETQLHHVLERHRNEGEQLIGKEAVKPE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
TIRVTHSADMQFVGQTHIINVPLPSSTVTRQELQALFEKAYFARFKVELPEIRANLVNLN
EEEEEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECHHHHEEEEECC
TSVTGVRPAIDLSRLIDPAGRAKTLDEARREIRPVWYAGQWHDTPVYAREKLPLDAVIEG
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHEECC
PAILEQMDATTVLEPGDRARSDADGNIIIDIGEA
HHHHHHCCCCEEECCCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure
MKENFSVQPPDSLGSVAAGIDVGGTFTDLLLIDGRDGGKVHIAKTPTTVDNQAFGVVSAL
CCCCCCCCCCHHHCCHHCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHH
GATGFAIDGIDLIVHGTTTTTNAVLERRLARTGMITTRGFRDVIELGRRTRPQPYGMTGT
CCCCEEECCEEEEEECCCCHHHHHHHHHHHHHCCEEECCHHHHHHHHHCCCCCCCCCCCC
FVPVIPRNLRLEVSERVEASGAVRTPLDEAEMREAVTALIAAGCESLVIHFLHSYANPAH
CCEECCCCEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHH
ERRAAEIAAELWPNGYITTGHALLSEAREFERGVTASVNASVQPILERYVERLRKELAAK
HHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
GYARDFLIMNGNGGMISARFVTRESAKTVMSGPASGVIAAAYTGKRAGFENLVTYDMGGT
CCCCEEEEECCCCCEEEEEEEEHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCC
STDVALIRNAEPAVSNEIEIEYAMPIHVPMVAVHTVGAGGGSIARVDAAGLIQIGPESAG
CCCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEECCCCEEEECCCCCC
ANPGPICYGRGGLEPTITDANLVLGRLAPKKLLAVDNPVTVERVTGIFADKIGKATGLSG
CCCCCEEECCCCCCCCCCCHHHHHHHCCCHHHEECCCCCCHHHHHHHHHHHHCCCCCCCC
VEAAGAVLRLGNMKMAGAIRMVSVSRGHDPRDFALFAFGGAGPLHATALARELGLPRVLV
HHHHHHHHEECCEEECCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEE
PARPGITNALGCVVADLRHDFVNTINQPVAVLDETQLHHVLERHRNEGEQLIGKEAVKPE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCC
TIRVTHSADMQFVGQTHIINVPLPSSTVTRQELQALFEKAYFARFKVELPEIRANLVNLN
EEEEEECCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECHHHHEEEEECC
TSVTGVRPAIDLSRLIDPAGRAKTLDEARREIRPVWYAGQWHDTPVYAREKLPLDAVIEG
CCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHEECC
PAILEQMDATTVLEPGDRARSDADGNIIIDIGEA
HHHHHHCCCCEEECCCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]