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The map label for this gene is pyrF

Identifier: 13473982

GI number: 13473982

Start: 3769204

End: 3769917

Strand: Reverse

Name: pyrF

Synonym: mll4751

Alternate gene names: 13473982

Gene position: 3769917-3769204 (Counterclockwise)

Preceding gene: 13473983

Following gene: 13473981

Centisome position: 53.58

GC content: 65.83

Gene sequence:

>714_bases
ATGCAGGCCAAATCCATCCAGGCCCAGTCCATCCAGGAAAGACTGATCGTCGGCCTCGACCTACCGACCGTGCGGGAAGC
CGAGCAGGCGGTGCGCGAACTCGACGGCGTCGTCTCCTTCTACAAGATCGGCTATCAGCTGGCTTTCGCCGGCGGGCTCG
ACTTTGCCAGGGAACTGGCCAGCGGCGGGACAAAGATCTTCCTCGACATGAAGCTGCTCGACATCGACCACACGGTGGCC
AAGGGCGTCGAGAACATCGTCAAGATGGGCATGACCATGCTGACCATCCACGCCTATCCCAAGGCGATGCGCGCGGCGGT
GGAAGCGGCCAGGGGCAGTGACCTTTGCCTGCTCGCCGTCAGCGTGCTGACCTCGATGGACGAGCAGGACATGATCGATG
TCGGCTATGAATATGACCCGCACACGCTGGTGCTGCGGCGCTCTGAACAAGCGCTGCATGCCGGCATGGGCGGCATCGTC
TGCTCGGCGGCGGAGGCAGAAGCGGTGCGCCGCATCGTTGGCCCCGACATGGCGGTGGTGACACCCGGCATCCGGCCGGC
CGGCAGCGACCATGGCGACCAGAAACGCGTGGTGACGCCGGCGCAAGCGATCCGCAACGGCGCCAGCCATCTTGTGGTGG
CCCGCCCGATCGTCGCGGCAAGCGACCGCCGCGCGGCGGCGCAGGCGATCCTCGACGAAATGCGCTCTGCATAG

Upstream 100 bases:

>100_bases
GTGACGAAAGACTATACAAATCGATGGAGCATTTATGCCGCACGCTGATCGAAACCTCGCGCATGCTGTCGACCCGGATC
GAGGCGTGACCGCCGCGACC

Downstream 100 bases:

>100_bases
AATAAGGGAGAGAACAATGCCCAAGGGATACTGGATCGCCCGCGTCGATGTGCGCGATGCCGAAGGCTACAAGGAGTATA
TCGCCGCTTCGAAGCTCGCC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MQAKSIQAQSIQERLIVGLDLPTVREAEQAVRELDGVVSFYKIGYQLAFAGGLDFARELASGGTKIFLDMKLLDIDHTVA
KGVENIVKMGMTMLTIHAYPKAMRAAVEAARGSDLCLLAVSVLTSMDEQDMIDVGYEYDPHTLVLRRSEQALHAGMGGIV
CSAAEAEAVRRIVGPDMAVVTPGIRPAGSDHGDQKRVVTPAQAIRNGASHLVVARPIVAASDRRAAAQAILDEMRSA

Sequences:

>Translated_237_residues
MQAKSIQAQSIQERLIVGLDLPTVREAEQAVRELDGVVSFYKIGYQLAFAGGLDFARELASGGTKIFLDMKLLDIDHTVA
KGVENIVKMGMTMLTIHAYPKAMRAAVEAARGSDLCLLAVSVLTSMDEQDMIDVGYEYDPHTLVLRRSEQALHAGMGGIV
CSAAEAEAVRRIVGPDMAVVTPGIRPAGSDHGDQKRVVTPAQAIRNGASHLVVARPIVAASDRRAAAQAILDEMRSA
>Mature_237_residues
MQAKSIQAQSIQERLIVGLDLPTVREAEQAVRELDGVVSFYKIGYQLAFAGGLDFARELASGGTKIFLDMKLLDIDHTVA
KGVENIVKMGMTMLTIHAYPKAMRAAVEAARGSDLCLLAVSVLTSMDEQDMIDVGYEYDPHTLVLRRSEQALHAGMGGIV
CSAAEAEAVRRIVGPDMAVVTPGIRPAGSDHGDQKRVVTPAQAIRNGASHLVVARPIVAASDRRAAAQAILDEMRSA

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=37.10407239819, Blast_Score=131, Evalue=4e-32,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_RHILO (Q98DD5)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105550.1
- ProteinModelPortal:   Q98DD5
- GeneID:   1228211
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4751
- NMPDR:   fig|266835.1.peg.3654
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25365; Mature: 25365

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 71-71 BINDING 20-20 BINDING 42-42 BINDING 124-124 BINDING 185-185 BINDING 194-194 BINDING 215-215

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAKSIQAQSIQERLIVGLDLPTVREAEQAVRELDGVVSFYKIGYQLAFAGGLDFARELA
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHH
SGGTKIFLDMKLLDIDHTVAKGVENIVKMGMTMLTIHAYPKAMRAAVEAARGSDLCLLAV
CCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SVLTSMDEQDMIDVGYEYDPHTLVLRRSEQALHAGMGGIVCSAAEAEAVRRIVGPDMAVV
HHHHCCCCCCHHHCCCCCCCCEEEEECCHHHHHCCCCHHEECHHHHHHHHHHHCCCCEEE
TPGIRPAGSDHGDQKRVVTPAQAIRNGASHLVVARPIVAASDRRAAAQAILDEMRSA
CCCCCCCCCCCCCCCCEECHHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAKSIQAQSIQERLIVGLDLPTVREAEQAVRELDGVVSFYKIGYQLAFAGGLDFARELA
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHH
SGGTKIFLDMKLLDIDHTVAKGVENIVKMGMTMLTIHAYPKAMRAAVEAARGSDLCLLAV
CCCCEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SVLTSMDEQDMIDVGYEYDPHTLVLRRSEQALHAGMGGIVCSAAEAEAVRRIVGPDMAVV
HHHHCCCCCCHHHCCCCCCCCEEEEECCHHHHHCCCCHHEECHHHHHHHHHHHCCCCEEE
TPGIRPAGSDHGDQKRVVTPAQAIRNGASHLVVARPIVAASDRRAAAQAILDEMRSA
CCCCCCCCCCCCCCCCEECHHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968