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The map label for this gene is 13473361

Identifier: 13473361

GI number: 13473361

Start: 3150432

End: 3151916

Strand: Direct

Name: 13473361

Synonym: mlr3932

Alternate gene names: NA

Gene position: 3150432-3151916 (Clockwise)

Preceding gene: 13473359

Following gene: 13473362

Centisome position: 44.78

GC content: 66.26

Gene sequence:

>1485_bases
ATGTCCTTTCAAAAACTCGACGACCTCGGCCACAAGCTCGAAGCGCTTGAGCATGCGCTGGCCATCCTCGGCGCCGACGA
AGCGACGCATATGGCCGTCGGCGGCGGCGAGAAGCGCGCCGAGGCGATGGCGGCACTGGCCGGCATGTACCATGCCAGGG
CGACCGCACCGGAAATCGCCGACTGGATCGCCGCCGCCGAAGGCGAGGCGCTCGACGACGAGCAGCGCGCGGCGCTCGGC
GAACTGCGGCGGCAATACACCAACCTGACCTGCCTGCCGGTCGAATTCGTCGAACGCCAGACGACGGCGCGCATGCGTTG
CGAGCAGCTGTGGCGCGACCTGCGCGCCAAGAACGACTGGGCGGGTTTCCAGCCGGCGCTCGAGGGCGTTGTGGCGCTGG
TGCGCGAAGAGGCGGCTATGCGCTCAGACGTGCTCGGCCTCGCCCCTTACGATGCGCTGATGGAGCAATTCGACCCCGGC
AACCGTACCGCCGACATCACCCCCGTCTTCGCCGACCTGAAAGCCTTCCTCAAGGGATTCGTGCCGGAGGCGCTGGCGAT
CCAGGAAGCGCGGCTGCGCAAGCATCCGCTGAAGCCGCTCTCGGGCACCTACGCGATCGACAAGCAGCGCGAGCTTGGCC
TTGCCATGATGGCGGCTGTCGGTTTCGACCTGACGCATGGCTCGCTGTCGGTGTCGCACCATCCCTTCTGCGGCGGCGTG
CCCAGCGACGTGCGCATCACCACCCGTTACAAGACATCGGACTTCCTGTCGGCGCTGATGGGCGTGCTGCACGAGACCGG
CCATGCGCTCTATGAACAGAACCTGCCGAAGGCGTGGTCGCACTGGCCGCTCGGCAAGGCGCGCGGCATGGCCGTGCATG
AAAGCCAGAGCCTGTTCGTCGAAAAGCAGATCGGCCGCAATCCCGCCTTCTGGCGCTGGGCCCTGCCGGTGGTGGAAAAA
CATCTCGGCGAAGCCTGGTCGCTCGACGACCTCCTGCCGCATGTGCATCGCGTCGAGCGCGGCCTGATCCGCGTCGACGC
CGACGAGGTGACCTATCCCCTGCATGTCATCCTGCGCTACGAGCTGGAGCAGGAGCTGGTCTCGGGCAGGCTGGAGGTTG
CCGATTTGCCCGAAGCCTGGGACGCCAAGATGCGCGACTATCTCGGTCTCTCCACGCTGGACAACCCGGCTGACGGGCCG
ATGCAGGACGTGCACTGGCCAGGCGCCGCCTTCGGCTACTTCCCGTCCTACACGCTGGGCGCGATGATGGCGGCGCAGCA
ATGGGCCGCGCTGACGCGCGAACATCCCACCGCCGATGACGACCTTGCCAAAGGAAACTTCGCGGCCATCAATGGATGGC
GCCGCGAAAAAATCTGGTCGCAGGGTTCGCGCTGGTCGACGCCGAACCTGCTCGAACGCGCCACCGGCGAAAAGCTCAAC
GCGGTGCATTTCGTCAATCACCTGAAGCAGCGCTACGGCGGATGA

Upstream 100 bases:

>100_bases
GAGCGGTTTGCCGTTTCCATGAAACGGTGAACTGCCCTGAGGGTTGCCAAGGCTTTTGCGTTGGTGTCTTTTCGCGCCGT
CCTCAATCACGGAAGCCGCC

Downstream 100 bases:

>100_bases
GATCGGCCGCCCCGGTTTCGGCGACCGGCGGCGGCGAACAAGGAGGCGATGACGCGCCATCGCCGGGCGGCGATCTTGCG
GGATCGCCGCTACTTTTCTA

Product: thermostable carboxypeptidase 1

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSFQKLDDLGHKLEALEHALAILGADEATHMAVGGGEKRAEAMAALAGMYHARATAPEIADWIAAAEGEALDDEQRAALG
ELRRQYTNLTCLPVEFVERQTTARMRCEQLWRDLRAKNDWAGFQPALEGVVALVREEAAMRSDVLGLAPYDALMEQFDPG
NRTADITPVFADLKAFLKGFVPEALAIQEARLRKHPLKPLSGTYAIDKQRELGLAMMAAVGFDLTHGSLSVSHHPFCGGV
PSDVRITTRYKTSDFLSALMGVLHETGHALYEQNLPKAWSHWPLGKARGMAVHESQSLFVEKQIGRNPAFWRWALPVVEK
HLGEAWSLDDLLPHVHRVERGLIRVDADEVTYPLHVILRYELEQELVSGRLEVADLPEAWDAKMRDYLGLSTLDNPADGP
MQDVHWPGAAFGYFPSYTLGAMMAAQQWAALTREHPTADDDLAKGNFAAINGWRREKIWSQGSRWSTPNLLERATGEKLN
AVHFVNHLKQRYGG

Sequences:

>Translated_494_residues
MSFQKLDDLGHKLEALEHALAILGADEATHMAVGGGEKRAEAMAALAGMYHARATAPEIADWIAAAEGEALDDEQRAALG
ELRRQYTNLTCLPVEFVERQTTARMRCEQLWRDLRAKNDWAGFQPALEGVVALVREEAAMRSDVLGLAPYDALMEQFDPG
NRTADITPVFADLKAFLKGFVPEALAIQEARLRKHPLKPLSGTYAIDKQRELGLAMMAAVGFDLTHGSLSVSHHPFCGGV
PSDVRITTRYKTSDFLSALMGVLHETGHALYEQNLPKAWSHWPLGKARGMAVHESQSLFVEKQIGRNPAFWRWALPVVEK
HLGEAWSLDDLLPHVHRVERGLIRVDADEVTYPLHVILRYELEQELVSGRLEVADLPEAWDAKMRDYLGLSTLDNPADGP
MQDVHWPGAAFGYFPSYTLGAMMAAQQWAALTREHPTADDDLAKGNFAAINGWRREKIWSQGSRWSTPNLLERATGEKLN
AVHFVNHLKQRYGG
>Mature_493_residues
SFQKLDDLGHKLEALEHALAILGADEATHMAVGGGEKRAEAMAALAGMYHARATAPEIADWIAAAEGEALDDEQRAALGE
LRRQYTNLTCLPVEFVERQTTARMRCEQLWRDLRAKNDWAGFQPALEGVVALVREEAAMRSDVLGLAPYDALMEQFDPGN
RTADITPVFADLKAFLKGFVPEALAIQEARLRKHPLKPLSGTYAIDKQRELGLAMMAAVGFDLTHGSLSVSHHPFCGGVP
SDVRITTRYKTSDFLSALMGVLHETGHALYEQNLPKAWSHWPLGKARGMAVHESQSLFVEKQIGRNPAFWRWALPVVEKH
LGEAWSLDDLLPHVHRVERGLIRVDADEVTYPLHVILRYELEQELVSGRLEVADLPEAWDAKMRDYLGLSTLDNPADGPM
QDVHWPGAAFGYFPSYTLGAMMAAQQWAALTREHPTADDDLAKGNFAAINGWRREKIWSQGSRWSTPNLLERATGEKLNA
VHFVNHLKQRYGG

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 55004; Mature: 54873

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQKLDDLGHKLEALEHALAILGADEATHMAVGGGEKRAEAMAALAGMYHARATAPEIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCCCHHHH
DWIAAAEGEALDDEQRAALGELRRQYTNLTCLPVEFVERQTTARMRCEQLWRDLRAKNDW
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGFQPALEGVVALVREEAAMRSDVLGLAPYDALMEQFDPGNRTADITPVFADLKAFLKGF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VPEALAIQEARLRKHPLKPLSGTYAIDKQRELGLAMMAAVGFDLTHGSLSVSHHPFCGGV
CCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCC
PSDVRITTRYKTSDFLSALMGVLHETGHALYEQNLPKAWSHWPLGKARGMAVHESQSLFV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEECCHHHHH
EKQIGRNPAFWRWALPVVEKHLGEAWSLDDLLPHVHRVERGLIRVDADEVTYPLHVILRY
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHH
ELEQELVSGRLEVADLPEAWDAKMRDYLGLSTLDNPADGPMQDVHWPGAAFGYFPSYTLG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
AMMAAQQWAALTREHPTADDDLAKGNFAAINGWRREKIWSQGSRWSTPNLLERATGEKLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCHHHHHHCCCHHH
AVHFVNHLKQRYGG
HHHHHHHHHHHHCC
>Mature Secondary Structure 
SFQKLDDLGHKLEALEHALAILGADEATHMAVGGGEKRAEAMAALAGMYHARATAPEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCHHHHHHHHHHHHHHHHHCCCHHHH
DWIAAAEGEALDDEQRAALGELRRQYTNLTCLPVEFVERQTTARMRCEQLWRDLRAKNDW
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AGFQPALEGVVALVREEAAMRSDVLGLAPYDALMEQFDPGNRTADITPVFADLKAFLKGF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VPEALAIQEARLRKHPLKPLSGTYAIDKQRELGLAMMAAVGFDLTHGSLSVSHHPFCGGV
CCHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCC
PSDVRITTRYKTSDFLSALMGVLHETGHALYEQNLPKAWSHWPLGKARGMAVHESQSLFV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEECCHHHHH
EKQIGRNPAFWRWALPVVEKHLGEAWSLDDLLPHVHRVERGLIRVDADEVTYPLHVILRY
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHH
ELEQELVSGRLEVADLPEAWDAKMRDYLGLSTLDNPADGPMQDVHWPGAAFGYFPSYTLG
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHH
AMMAAQQWAALTREHPTADDDLAKGNFAAINGWRREKIWSQGSRWSTPNLLERATGEKLN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCHHHHHHCCCHHH
AVHFVNHLKQRYGG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]